Addgene

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pcDNA4-TO-Hygromycin-mVenus-MAP

Plasmid

#44099

Depositor

Dannel McCollum

Article

Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

Type

Empty backbone

Use

Tags

mVenus-MAP

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pVF53

Plasmid

#229477

Purpose

expresses RssBE229C

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (E229C)

Use

Tags

Expression

Bacterial

Mutation

E229C

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pBD1

Plasmid

#229463

Purpose

Expresses RssB

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF30

Plasmid

#229472

Purpose

Expresses RssB with R115A mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (R115A)

Use

Tags

Expression

Bacterial

Mutation

R115A

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF29

Plasmid

#229473

Purpose

Expresses RssB with N256A mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (N256A)

Use

Tags

Expression

Bacterial

Mutation

N256A

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF28

Plasmid

#229469

Purpose

Expresses RssB with D104A mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (D104A)

Use

Tags

Expression

Bacterial

Mutation

D104A

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF31

Plasmid

#229468

Purpose

Expresses RssB with Q254A mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (Q254A)

Use

Tags

Expression

Bacterial

Mutation

Q254A

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF16

Plasmid

#229465

Purpose

Expresses RssB with D58P mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (D58P)

Use

Tags

Expression

Bacterial

Mutation

D58P

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMS11

Plasmid

#229464

Purpose

Expresses RssB with His Tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB

Use

Tags

10xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVF14

Plasmid

#229455

Purpose

Expresses IraD with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVF17

Plasmid

#229454

Purpose

Expresses IraM with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraM

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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H2B-MiniR

Plasmid

#208328

Purpose

MiniR genetically encoded tag introduced after H2B; to be used with a MiniE probe peptide.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

H2B-MiniR

Use

Tags

MiniR

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-PuRRRE

Plasmid

#208327

Purpose

C-terminal PuRRRE-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-PuRRRE

Use

Tags

PuRRRE

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-TREER

Plasmid

#208325

Purpose

C-terminal TREER-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-TREER

Use

Tags

TREER

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-TinyERRE

Plasmid

#208324

Purpose

C-terminal TinyERRE-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-TinyERRE

Use

Tags

TinyERRE

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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TREER-Actin

Plasmid

#208330

Purpose

TREER genetically-encoded tag introduced before actin; to be used with TinyERRE probe peptide.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TREER-Actin

Use

Tags

TREER

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TOMM20-PuRRRE

Plasmid

#208329

Purpose

PuRRRE genetically-encoded tag introduced after TOMM20; to be used with PunyEEER probe peptide.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TOMM20-PuRRRE

Use

Tags

PuRRRE

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-PunyEEER

Plasmid

#208326

Purpose

C-terminal PunyEEER-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-PunyEEER

Use

Tags

PunyEEER

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pJH1973

Plasmid

#178903

Purpose

Psra-11::D3cpv unc-54 3' UTR C.elegans neural expression of Psra-11 D3cpv

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

D3cpv

Use

Tags

Expression

Worm

Mutation

Promoter

Psra-11

Available since

Jan. 5, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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Villinpromoter-blue-FlpOERT2

Plasmid

#67278

Purpose

4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context

Depositor

Jannik Elverløv-Jakobsen

Article

Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do

Insert

mTQ2=mturquoise2 blue fluorescent gene

Use

Tags

linked to FlpO through an P2A ribosomal skipping …

Expression

Mutation

Promoter

Villin

Available since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

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