Addgene

We narrowed to 11,157 results for: cel.2

Showing: 3561 - 3580 of 11157 results

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pHyg-CUbo(K48R/K63R)-yeGFP

Plasmid

#212769

Purpose

C-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCF821-sgRPA1-2_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211659

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgRPA1-2 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCF821-sgOR2B6-2_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211656

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgOR2B6-2 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCF821-sgCIDE-2_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211662

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-2 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCF820-sgCIDE-2_U6-sgRNA-EF1a-mCherry2

Plasmid

#211650

Purpose

U6-sgRNA-EF1a-mCherry2

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

sgCIDE-2 sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCF821-sgNT-2_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211653

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgNT-2 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCaUra3-CUbo(K48R/K63R)-yeGFP

Plasmid

#212774

Purpose

C-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP in budding yeast with CaUra3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pHyg-CUbo(K48R)-yeGFP-His6

Plasmid

#212783

Purpose

C-terminal PCR-based tagging with CUbo(K48R)-yeGFP-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo(K63R)-yeGFP-His6

Plasmid

#212784

Purpose

C-terminal PCR-based tagging with CUbo(K63R)-yeGFP-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo(K48R/K63R)-9myc

Plasmid

#212789

Purpose

C-terminal PCR-based tagging with CUbo(K48R/K63R)-9myc in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pUra3-pCUP1-CUbo(K48R/K63R)

Plasmid

#212765

Purpose

N-terminal PCR-based tagging with pCUP1-CUbo(K48R/K63R) in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a-His6-CUbo(K63R)-MCS

Plasmid

#212727

Purpose

Bacterial expression of a protein of interest with N-terminal His6-CUbo(K63R)-fusion, contains multiple cloning site

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET24a-His6-CUbo(K48R)-MCS

Plasmid

#212726

Purpose

Bacterial expression of a protein of interest with N-terminal His6-CUbo(K48R)-fusion, contains multiple cloning site

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b-His6-CUbo-GFP

Plasmid

#212720

Purpose

Bacterial expression of His6-CUbo-GFP

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

GFP

Use

Tags

6xHis-CUbo

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a-His7-NUbo-GFP

Plasmid

#212719

Purpose

Bacterial expression of His7-NUbo-GFP

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

GFP

Use

Tags

7xHis-NUbo

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a-NUbo-GFP-His6

Plasmid

#212718

Purpose

Bacterial expression of NUbo-GFP-His6

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

GFP

Use

Tags

6xHis and NUbo

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET30-His6-TEV-Mms2

Plasmid

#212716

Purpose

Bacterial expression of His6-Mms2, His6-tag cleavable with TEV-protease

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

Mms2

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pHR V5-RNF25 dRING_IRES-mCherry

Plasmid

#198387

Purpose

RNF25 (?RING) expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RNF25 (RNF25 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

RING deletion (135-201)

Promoter

EF1A

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pFX-H2B-Sirius

Plasmid

#175837

Purpose

Express Sirius with nucleus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with nucleus-targeting sequence

Use

Tags

H2B (human histone 2B)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-iRFP-Rab5

Plasmid

#174456

Purpose

Express iRFP with early endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with early endosome-targeting sequence

Use

Tags

small GTPase (Rab5)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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