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Plasmid#62901PurposeCreating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 2DepositorInsertT2A-Gal4
UseTagsExpressionMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Med29 pBacPAK8
Plasmid#15369DepositorInsertMed29 (MED29 Human)
UseBaculovirus constructionTagsFLAGExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pAc5.1B-lambdaN-HA-DmeIF4G-trunc-V5His6_G
Plasmid#146392PurposeInsect Expression of DmeIF4G-trunc. *Note: this plasmid does not contain an HA-tag as the name implies.DepositorInsertDmeIF4G-trunc (eIF4G1 Fly)
UseTagsExpressionInsectMutation322-1666 truncated version of elF4G sequence with…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP in pDBEcR-pIND(SP1)-DD/Bla
Plasmid#201559PurposeEcdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD and DBEcR (3xmyc-tagged); blasticidin resistance.DepositorInsertDBEcR and EGFP
UseUnspecified; Sleeping beauty transposonTagsDBEcR-myc, FLAG-EGFP, and FLAG-EGFP-DDExpressionMammalianMutationPromoterEF1a and pIND(SP1)Available sinceJuly 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP in pDBEcR-pIND(SP1)-W/Bla
Plasmid#201558PurposeEcdysone-inducible vector with SB ITRs for expressing FLAG- EGFP (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.DepositorInsertDBEcR and EGFP
UseUnspecified; Sleeping beauty transposonTagsDBEcR-myc and FLAG-EGFPExpressionMammalianMutationPromoterEF1a and pIND(SP1)Available sinceJuly 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS hIhh (CT#402)
Plasmid#13995DepositorInsertIhh (IHH Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB
Plasmid#52890Purpose"CoinFLP-LexGAD/Gal4" - Expresses LexGAD or Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express Gal4.DepositorInsertsUseTagsExpressionInsectMutationPromoterAvailable sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-Hel25E WT
Plasmid#110123PurposeExpresses Flag-tagged D. melanogaster Hel25EDepositorInsertHel25E (Hel25E Fly)
UseTagsFlagExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceOct. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) Laccase2 MCS-ciRS7 Exon
Plasmid#69900PurposeExpresses the human ciRS7/CDR1as circular RNA in mammalian cellsDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
K401-Micro
Plasmid#122485PurposeComponent of optically-induced motor dimerization systemDepositorInsertHis tag MBP Kinesin 1-401 SspB micro
UseTagsH6-MBP-TEV and SspB microExpressionBacterialMutationKinesin amino acids 1-401PromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUAST-SIK3 T196E
Plasmid#52975Purposeinsect expression of SIK3 T196E constitutively active mutantDepositorInsertSIK3 (Sik3 Fly)
UseTagsHAExpressionInsectMutationT196E constitutively active mutantPromoterHsp70Available sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST-SIK3 WT
Plasmid#52972Purposeinsect expression of SIK3 WTDepositorInsertSIK3 (Sik3 Fly)
UseTagsHAExpressionInsectMutationPromoterHsp70Available sinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPuroR-MCS-LexA-VP16-MiniWhite
Plasmid#165898PurposePuromycin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloningDepositorInsertSelectable Empty Enhancer Driver
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS mEya1 (CT#671)
Plasmid#13971DepositorInsertEya1 (Eya1 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pUAST-SIK3 K70M
Plasmid#52973Purposeinsect expression of SIK3 K70M (kinase dead mutant)DepositorInsertSIK3 (Sik3 Fly)
UseTagsHAExpressionInsectMutationK70M kinase dead mutantPromoterHsp70Available sinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR-attL5-CRY2-mCh-attL2
Plasmid#160438PurposeEntry vector for cloning Cryptochrome2-mCherry using 2-fragment gateway recombinationDepositorInsertCryptochrome 2 (CRY2 Mustard Weed)
UseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
RCAS(A) dRhoA V14 (CT#846)
Plasmid#13949DepositorInsertRhoA (Rho1 Fly)
UseRetroviral; Avian expressionTagsExpressionMutationG14V mutationPromoterAvailable sinceFeb. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pC-(loxP2-attB2-SA(0)-T2A-Gal4-Hsp70)
Plasmid#62954PurposeSinglet donor (phase 0) for in vivo RMCE with MiMIC inserts to make Gal4 driver.DepositorInsertT2A-Gal4-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC-(loxP2-attB2-SA(1)-T2A-Gal4-Hsp70)
Plasmid#62955PurposeSinglet donor (phase 1) for in vivo RMCE with MiMIC inserts to make Gal4 driver.DepositorInsertT2A-Gal4-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only