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pCS2z-tal1

Plasmid

#164656

Purpose

for the synthesis of zebrafish tal1 mRNA

Depositor

Didier Stainier

Article

Mattonet et al Sci Adv. 2022 Sep 2;8(35):eabn2082. doi:

Insert

tal1 (tal1 Zebrafish)

Use

Mrna synthesis

Tags

Expression

Mutation

Promoter

SP6

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pGWB421

Plasmid

#74815

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 10xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pMM627

Plasmid

#112532

Purpose

pZE2-PLhrtO-lux-hrtR-RBS2-chuA - Composite plasmid of pMM534 and pMM549, ColE1 origin, KanR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Inserts

HrtR
ChuA
luxCDABE

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23107, PL(HrtO), and ProD

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pGWB524

Plasmid

#74866

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with GST (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB529

Plasmid

#74871

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with TAP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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PLEX_313 GFP

Plasmid

#184494

Purpose

Lentivirus expression for GFP

Depositor

Todd Golub, Peter Tsvetkov

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGAN200

Plasmid

#129203

Purpose

yTRAP sensor of [PSI+], detects aggregation of Sup35

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

Sup35NM (SUP35 Budding Yeast)

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

N terminal and middle domains

Promoter

SUP35

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGWB443

Plasmid

#74829

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with eCFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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p53-(1-393)-R175H-N268D

Plasmid

#35086

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

p53 (TP53 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains aa 1-393 - R175H-N268D

Promoter

T7

Available since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
pGWB409

Plasmid

#74803

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 6xHis (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pSK267

Plasmid

#129272

Purpose

"ZF only" yTRAP control to control for yTRAP reporter fluorescence changes not dependent on sensed protein

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

NLS-VP16-ZF 43-8-NES

Use

Tags

6xHA

Expression

Yeast

Mutation

Promoter

SUP35

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCC20

Plasmid

#198783

Purpose

Drives specific expression in the ASK neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Psra-9-GAL4-SK(DBD)-VP64

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB528

Plasmid

#74870

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with TAP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB419

Plasmid

#74813

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB522

Plasmid

#74864

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with GST (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pME-nls-sfGFP

Plasmid

#132973

Purpose

full-length of nuclear localized sfGFP with stop codon in pmE Gateway vector

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Insert

nuclear localized super folder GFP

Use

Gateway vector

Tags

6x myc and SV40 NLS

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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TC1092

Plasmid

#164879

Purpose

CMV-dcas13d-NLS-ADARdd- mCherry

Depositor

Tian Chi

Article

Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:

Insert

dcas13d-ADARdd

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGWB509

Plasmid

#74851

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 6xHis (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pCS-foxc1a-2a-nlstdTomato

Plasmid

#132970

Purpose

full-length of foxc1a in frame with viral 2a peptide and nuclear localized tdTomato in pCS expression vector

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Inserts

tdTomato
foxc1a (foxc1a Zebrafish)

Use

Tags

NLS and myc

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pGWB527

Plasmid

#74869

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with T7 (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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