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pDONR223-HK1

Plasmid

#23730

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

HK1 (HK1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pGWB402-omega

Plasmid

#74797

Purpose

Gateway cloning compatible binary vector for expression of gene by 2xCaMV35S enhancer and omega sequence.

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

2xCaMV35S enhancer and omega

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Notch1 intracellular domain-pcw107-V5

Plasmid

#64622

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH1 (NOTCH1 Human)

Use

Lentiviral

Tags

V5

Mutation

intracellular domain *

Promoter

PGK

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
PP1 7-300

Plasmid

#26566

Depositor

Wolfgang Peti

Publication

Kelker et al J Mol Biol. 2009 Jan 9. 385(1):11-21.

Insert

PP1a (PPP1CA Human)

Tags

His6, TEV cleavage site, and Thio-6

Expression

Bacterial

Mutation

Contains only aa 7-330

Available since

Dec. 16, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles
pSkylan-NS-N1

Plasmid

#86785

Purpose

for fusion of gene of interest with a monomeric reversibly photoswitchable fluorescent protein, Skylan-NS

Depositor

Pingyong Xu

Publication

Zhang et al Proc Natl Acad Sci U S A. 2016 Sep 13;11

Type

Empty backbone

Tags

Skylan-NS

Expression

Mammalian

Promoter

CMV

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pVITRO1-dV-IgE/κ

Plasmid

#61878

Purpose

Human immunoglobulin epsilon antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

Depositor

Andrew Beavil

Publication

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

Human immunoglobulin Epsilon constant region
Human immunoglobulin kappa constant region

Expression

Mammalian

Mutation

Deleted Variable region

Promoter

mEF1 Prom and rEF1 Prom

Available since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA-FLAG-METTL3-APPA

Plasmid

#160251

Purpose

Express catalytic inactive FLAG-METTL3 in human cells

Depositor

Alessandro Fatica

Publication

Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

Insert

methyltransferase like 3 (METTL3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changes in aa 395–398, DPPW → APPA

Promoter

CMV

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGWB555

Plasmid

#74885

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pKanCMV-mRuby3-18aa-actin

Plasmid

#74255

Purpose

Mammalian expression of N-terminal fusion of mRuby3 to actin

Depositor

Michael Lin

Publication

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

actin

Tags

mRuby3

Expression

Mammalian

Promoter

CAG and CMV

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Dendra2-Actin-C-18

Plasmid

#57701

Purpose

Localization: Actin, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Publication

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Actin (ACTB Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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pTol1-UAS:CoChR-tdTomato

Plasmid

#124233

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Publication

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

CoChR-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Promoter

14xUAS-E1b

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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iRFP-C1

Plasmid

#54786

Purpose

Localization: C1 Cloning Vector, Excitation: 690, Emission: 713

Depositor

Michael Davidson, Vladislav Verkhusha

Publication

Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

Type

Empty backbone

Tags

iRFP

Expression

Mammalian

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

6 Articles

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Sirius/pRSETB

Plasmid

#51956

Purpose

bacterial expression of Sirius, a pH-insensitive and photostable ultramarine fluorescent protein

Depositor

Takeharu Nagai

Publication

Tomosugi et al Nat Methods. 2009 May;6(5):351-3. doi: 1

Insert

Sirius

Tags

6xHis

Expression

Bacterial

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRN110

Plasmid

#84462

Purpose

pJB38-NWMN29-30 + SarA_P1-mKate2-Term

Depositor

Reindert Nijland

Publication

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-mKate2 from pRN11

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pTY-EF1a-wild type mKdm2b-Flag

Plasmid

#61739

Purpose

Express wild-type mouse histone demethylase Kdm2b

Depositor

Yi Zhang

Publication

He et al Nat Cell Biol. 2013 Apr;15(4):373-84. do

Insert

Kdm2b (Kdm2b Mouse)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pCDNA5-Flag-BRD4-BD

Plasmid

#90005

Purpose

expresses BD mutant BRD4

Depositor

Kornelia Polyak

Publication

Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed 140N to 140A, 433N to 433A

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pKanCMV-mClover3-18aa-actin

Plasmid

#74259

Purpose

Mammalian expression of N-terminal fusion of mClover3 to actin

Depositor

Michael Lin

Publication

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Actin

Tags

mClover3

Expression

Mammalian

Promoter

CAG and CMV

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pFA6a-13Myc-kanMX6

Plasmid

#39294

Depositor

Jurg Bahler, John Pringle

Publication

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

KanR

Use

Yeast genomic targeting

Tags

13Myc, A. gossypii translation elongation factor …

Available since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pFG2

Plasmid

#87250

Purpose

gene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT). Also known as pANCH1-Nat

Depositor

Kerstin Bystricky

Publication

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

Nat

Tags

parS segments

Expression

Yeast

Promoter

TEF

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAGM8031

Plasmid

#48037

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

4 Articles

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