We narrowed to 27,806 results for: sta

1. pCDNA5_FRT_TO_TurboID-mCherry-Cry2

   Plasmid

   #166504

   Purpose

   Expression of your protein of interest fused with TurboID and mcherry-CRY2 for optogenetic activation, Flp-In T-Rex system. Search for client proteins within light-induced biomolecular condensates.

   Depositor

   Angelos Constantinou

   Article

   Frattini et al Mol Cell. 2021 Jan 16. pii: S1097-2765(2

   Insert

   None
   

   Tags

   TurboID and mCherry-CRY2

   Expression

   Mammalian

   Available Since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVSL10

   Plasmid

   #201182

   Purpose

   Bst polymerase large fragment (BF) F710Y/D598A, His6-tagged, HRV 3C-cleavable

   Depositor

   Jack Szostak

   Article

   Lelyveld et al Science. 2023 Oct 27;382(6669):423-429.

   Insert

   Bst polymerase large fragment (BF) F710Y/D598A
   

   Tags

   HRV 3C protease cleavage site and His6

   Expression

   Bacterial

   Mutation

   F710Y D598A

   Promoter

   T5-lacO

   Available Since

   Sept. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)/AUG-nLuc-3XFLAG

   Plasmid

   #127299

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNAintron-CHIKV-Envelope protein

   Plasmid

   #215700

   Purpose

   Produces the Chikungunya virus envelope protein E3-E2-6K-E1

   Depositor

   Melinda Brindley

   Article

   Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

   Insert

   CHIKV E3-E2-6K-E1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pGex-4T-2_GABARAP

   Plasmid

   #73948

   Purpose

   For recombinant expression of human GABARAP in E.coli

   Depositor

   Dieter Willbold

   Article

   Stangler et al J Biomol NMR. 2001 Oct;21(2):183-4.

   Insert

   GABARAP (GABARAP Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pTS2340_Tier3(SB)-iRFP_Puro

   Plasmid

   #169638

   Purpose

   Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PRPBSA-driven iRFP670 and PuroR expression
   

   Expression

   Mammalian

   Promoter

   PRPBSA

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMB1-A-TelR

   Plasmid

   #204979

   Purpose

   Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

   Depositor

   Veronica Godoy-Carter

   Article

   Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Added replications elements for Acinetobacter spe…

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLP_mod_mNeonGreen11_P2A_puroR_CRE-GFP

   Plasmid

   #229227

   Purpose

   Modular BxB1 landing pad vector containing C-term mNeonGreen11 tag, puroR marker, and a cAMP GFP reporter.

   Depositor

   Willow Coyote-Maestas

   Article

   Howard et al Cell. 2025 Feb 6;188(3):671-687.e20. doi

   Type

   Empty backbone

   Tags

   mNeonGreen11

   Expression

   Mammalian

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-C1-Nup35-mut

   Plasmid

   #87341

   Purpose

   To express human Nup35 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   NUP35 (NUP35 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   shRNA resistant changes

   Promoter

   CMV

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-ALK7 T194D

    Plasmid

    #80886

    Purpose

    mammalian expression of ALK7 T194D

    Depositor

    Aristidis Moustakas

    Article

    Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

    Insert

    ALK7 (Acvr1c Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T194D (constitutively active)

    Promoter

    CMV

    Available Since

    Aug. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pZS*11_4enh

    Plasmid

    #149423

    Purpose

    Plasmid harboring four plant enhancers (35S, AB80, Cab-1, and rbcS-E9).

    Depositor

    Stan Fields

    Article

    Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

    Inserts

    rbcS-E9 enhancer
    35S core enhancer
    Cab-1 enhancer
    AB80 enhancer
    

    Tags

    T120>A

    Expression

    Bacterial

    Available Since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA6a-GFP (F64L, S65T, V163A)-TRP1

    Plasmid

    #53184

    Purpose

    chromosomal tagging with stable GFP

    Depositor

    John Pringle

    Article

    Ko et al Mol Biol Cell. 2007 Dec;18(12):5139-53.

    Insert

    TRP1 (TRP1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    GFP (F64L, S65T, V163A)

    Available Since

    May 27, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pZA26

    Plasmid

    #158504

    Purpose

    In planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V-4xMyc. Km plant selection marker.

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    ZAR1-4xMyc (ZAR1 Mustard Weed)
    

    Tags

    4xMyc

    Expression

    Plant

    Mutation

    D489V

    Available Since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMBA334

    Plasmid

    #214746

    Purpose

    High copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23119-RiboJ-RBS(21992)-gfpmut3
    

    Expression

    Bacterial

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS1ClacZ

    Plasmid

    #55171

    Purpose

    lacZ-reporter vector, integration at amyE, ampr, cmr

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Bacillus biobrick box

    Promoter

    none

    Available Since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pLC133

    Plasmid

    #198639

    Purpose

    Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.

    Depositor

    David Bikard

    Article

    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

    Insert

    dCas9
    

    Tags

    3x-FLAG

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pATmin-GG

    Plasmid

    #191352

    Purpose

    Clostridium expression vector (pAMB1 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sites

    Depositor

    Andrew Tolonen

    Article

    Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

    Insert

    Plac-RFP (IPTG-inducible red fluorescent protein)
    

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Dual opposing T7 promoters reporter plasmid

    Plasmid

    #156457

    Purpose

    A mutational reporter plasmid that can gain Kanamycin resistance upon mutation. Contains two T7 promoters flanking the kanamycin resistance gene.

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

    Insert

    NeoR/KanR
    

    Expression

    Bacterial

    Mutation

    ATG start codon mutated to ACG

    Promoter

    Em7

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. FLAG SMG1 D2335A/N2340K

    Plasmid

    #199575

    Purpose

    Expresses FLAG-tagged kinase dead SMG1

    Depositor

    Michael Kastan

    Article

    McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

    Insert

    SMG1 (SMG1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D2335A and N2340K

    Promoter

    CMV

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-phsyn-FLEX-KASH-EGFP-U6-shRNA

    Plasmid

    #129708

    Purpose

    Express cre-dependent KASH-EGFP and scramble shRNA

    Depositor

    Yi Zhang

    Article

    Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

    Insert

    KASH-EGFP
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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