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Showing: 3541 - 3560 of 25548 results

  2. pET23b-2E2-HA-mEGFP-6xHis

    Plasmid
    #129594
    Purpose
    The encoded protein is the anti-HA frankenbody variant fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
    Use
    Tags
    6xHis-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    129594-plasmid-map-sequence-id-261157

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  4. pDIRECT_23C

    Plasmid
    #91140
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:bar
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91140-plasmid-map-sequence-id-182268

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  6. pICE-FLAG-NAT10-siR-G641E

    Plasmid
    #59366
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of G641E mutant of human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Steve Jackson
    Article
    Larrieu et al Science. 2014 May 2;344(6183):527-32. do
    Insert
    NAT10 NM_024662 (NAT10 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    G641E and silent mutations to render the cDNA res…
    Promoter
    CMV-tet
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    59366-plasmid-map-sequence-id-92067

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  11. pCMV-2E2-HA-mEGFP

    Plasmid
    #129595
    Purpose
    The encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    129595-plasmid-map-sequence-id-254267

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  14. MSP1830

    Plasmid
    #70708
    Purpose
    Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Insert
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    CAG
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    70708-plasmid-map-sequence-id-364684

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  17. MTOR-F2108L_CD19-CAR-28z-2A-EGFP_AAV6_Donor

    Plasmid
    #211904
    Purpose
    Vector for AAV6 donor production. Targeted CD19-CAR-28z-2A-EGFP transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.
    Depositor
    Yannick Doyon
    Article
    Levesque et al bioRxiv 2023.09.14.557485
    Insert
    CD19-CAR-28z-2A-EGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK1
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    211904-plasmid-map-sequence-id-420112

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  18. pS148∙CsR

    Plasmid
    #197858
    Purpose
    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria
    Depositor
    Susanne Häußler
    Article
    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41
    Insert
    gRNA scaffold - Ap/Cb resistance cassette - incP oriT
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    PEM7
    Available since
    Oct. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197858-plasmid-map-sequence-id-393255

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Showing: 3541 - 3560 of 25548 results