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Plasmid#60605PurposeKnock-in donor vector to insert Exon 44 in front of Exon 45 of human DYSTROPHIN (DMD)gene, include EF1a-hygromycin resistance cassette flanked by loxP sequences.DepositorInsertHygromycin resistant gene
UseKnock-in donor vectorExpressionMammalianAvailable sinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mTagBFP2-Endosomes-14
Plasmid#55293PurposeLocalization: Endosomes, Excitation: 399, Emission: 456DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEndosomes (RHOB Human)
TagsmTagBFP2ExpressionMammalianPromoterCMVAvailable sinceOct. 7, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223-ZAK
Plasmid#23709DepositorInsertZAK (MAP3K20 Human)
UseGateway CloningAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MAP2K7
Plasmid#23798DepositorAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SW5
Plasmid#40108DepositorPublicationInsertBistable switch using dynamic degradation
UseSynthetic BiologyExpressionBacterialAvailable sinceSept. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046757
Plasmid#145657PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1D/mEGFP + CrPV IGR IRES-nLuc-3XFLAG-CL1PEST
Plasmid#127332PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CrPV IRESDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnanoLuciferase
Tags3xFLAG and CL1/PESTExpressionMammalianPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV-tetO-Ets2
Plasmid#70272PurposeLentiviral plasmid for tetracycline/doxycycline dependent expression of murine Ets2DepositorAvailable sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFB2D
Plasmid#195286PurposeBicistronic DTR-EGFP fusion and Puromycin resistance for positive/negative selectionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDTR EGFP 2A Puro
ExpressionMammalianPromoterEFSAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-1-LARP7
Plasmid#113545PurposeProduces an N-terminal GST-tagged full length LARP7 protein.DepositorAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BlastR-P2A-DD-TCOF1-donor
Plasmid#60748PurposeDonor plasmid for HR-mediated recombination to insert blasticidin resistance and append a destabilization domain to the N-terminus of TCOF1DepositorInsertTCOF1 (TCOF1 Human)
UseTargeting donorAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-TGFBR2
Plasmid#23623DepositorInsertTGFBR2 (TGFBR2 Human)
UseGateway CloningAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJ2072.2_CRISPRlator
Plasmid#140674PurposeCRISPRlatorDepositorInsertCRISPRlator
UseSynthetic BiologyExpressionBacterialAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDA_886
Plasmid#201162PurposeLentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' VP64 and NLSDepositorInsertPuromycin resistance
UseCRISPR and LentiviralPromoterEFSAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE52
Plasmid#90397PurposeSTAT1 - STAT1 gene reporterDepositorInsertFirefly luciferase
UseLentiviralPromoterSTAT1Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
dCas9-5xGCN4-P2A-BFP
Plasmid#184438PurposedCas9 fused with 5 copies of GCN4 epitopeDepositorInsertdCas9-5xGCN4
UseCRISPR and LentiviralExpressionMammalianAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Flag-RagC(S75N)
Plasmid#99724PurposeMammalian expression of Flag-RagC(S75N)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRagC (RRAGC )
TagsFlagMutationS75NPromoterCMVAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
8xGliBS-IVS2-mCherry-NLS-polyA-Tol2
Plasmid#84604PurposeTol2-based hedgehog signal reporter plasmid for zebrafish transgenesisDepositorInsertmCherry
UseZebrafish transgenesisPromoter8xGliBS-delta-crystallin minimal promoterAvailable sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMS-eGFP-RTP801
Plasmid#65057PurposeExpression of RTP801 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDDIT4 (Ddit4 Rat)
TagsEGFPExpressionMammalianPromoterCMV-IEAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMB1_1R26PylRS(CbzK)_AfTyrRS(p-I-Phe)_AlvtRNA-ΔNPyl(8)(CGA)_AftRNA-Tyr(A01)(CUA)
Plasmid#174516PurposeRecoded double aaRS/tRNA plasmid with 1R26PylRS/AlvtRNA-ΔNPyl(8)(CGA) & AfTyrRS/AftRNA-Tyr(A01)(CUA) to incorporate CbzK into the TCG codon & p-I-Phe into the TAG codon.DepositorInsertsPylS_1R26 - CBZK
Af_TyrRS - p-I-Phe
Ma tRNA pylT(8) with CGA anticodon
A.fulgidus tRNA with CUA anticodon
ExpressionBacterialMutationCUA anticodon, M.alvus Pyl tRNA with CGA anticodo…PromoterGlnS and lppAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only