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Plasmid#198421PurposepBs3:RedNTF:tNOS. A pGreen based plasmid expressing the INTACT reporter RedNTF after the promoter of the pepper Bs3 gene (pBs3). The plasmid has spectinomycin resistance.DepositorInsertRedNTF
UseTagsExpressionPlantMutationPromoterpBs3Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_TOMM20_HaloTag9_T2A_EGFP
Plasmid#169333PurposeExpression of HaloTag9 at the mitochondrial surface of mammalian cellsDepositorInsertTOMM20-HaloTag9 (TOMM20 Human)
UseTagsHaloTag9ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21a-C2-mKate
Plasmid#208763PurposeTo express C2-mKate in bacteriaDepositorInsertC2-mKate (Mfge8 Mouse)
UseTagsmKateExpressionBacterialMutationPromoterT7 promoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX Tec-SH2
Plasmid#46522DepositorInsertTec (TEC Human)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 236-351PromoterTacAvailable sinceOct. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
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Adeno EA
Plasmid#64071PurposeAdenovirus for the expression of gRNAs targeting intron 19 of murine Alk and intron 14 of Eml4DepositorInsertU6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9
UseAdenoviralTagsFLAG-Cas9ExpressionMutationPromoterU6 and CBhAvailable sinceJuly 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_14
Plasmid#194675PurposeProtein production of the split HaloTag based calcium recorder Caprola_14 in bacteriaDepositorInsertCaprola_14
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_15
Plasmid#194676PurposeProtein production of the split HaloTag based calcium recorder Caprola_15 in bacteriaDepositorInsertCaprola_15
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHS1929
Plasmid#205968PurposeJAGBLH010000220 Cas8-HNH crRNA expression in pCOLADuet-1DepositorInsertCas8-HNH crRNA
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-PGAM1_sgRNA1
Plasmid#201614PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertPGAM1 (PGAM1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Caprola_04-mEGFP
Plasmid#194682PurposeCMV driven expression of the calcium recorder Caprola_04 in mammalian cell lines fused to mEGFPDepositorInsertCaprola_04-mEGFP
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-deLACCO1-NGR
Plasmid#208024PurposeControl biosensor for extracellular L-lactateDepositorInsertdeLACCO1
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5 GFP RagB Q99L
Plasmid#112749Purposeexpress GFP-RagB (Q99L mutant)DepositorInsertGFP-RagB (Q99L) (RRAGB Human)
UseTagseGFPExpressionMammalianMutationQ99LPromoterAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P shFXN_1
Plasmid#102970PurposeSuppression of FXNDepositorInsertshFXN_1 (FXN Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRB2 CTF-FLAG
Plasmid#217682PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRB2 (BAI2 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-911PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
CRY2PHR(W349R)-mCherry
Plasmid#75370PurposeExpresses fusion of Arabidopsis CRY2 (residues 1-498, W349R fast-cycling mutant) fused to mCherryDepositorInsertCRY2 residues 1-498 (CRY2 Mustard Weed)
UseTagsmCherryExpressionMammalianMutationW349RPromoterCMVAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTol2_elavl3(HuC)_NES-Caprola_01-mEGFP
Plasmid#194696PurposeHuC driven expression of the calcium recorder Caprola_01 fused to mEGFP for neuronal expression in zebrafishDepositorInsertCaprola_01-mEGFP
UseZebrafish expressionTagsmEGFPExpressionMutationPromoterelavl3(HuC)Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHS1068
Plasmid#205962PurposeNZ_CP025815 DinG HNH proteins (E. coli codon optimized) and CRISPR array in pACYCDuet-1 with Lac promotersDepositorInsertDinG HNH proteins and CRISPR array
UseTagsExpressionBacterialMutationPromoterLac (proteins), pJ23119 (crRNA)Available sinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P shFXN_2
Plasmid#102971PurposeSuppression of FXNDepositorInsertshFXN_2 (FXN Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDule2-IBBN (G2)
Plasmid#85501PurposePlasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertIBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
UseTagsExpressionBacterialMutationY32G L65E F108W Q109M D158S R162KPromoterlpp (constitutive)Available sinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only