Addgene

We narrowed to 11,285 results for: pcDNA

Showing: 3521 - 3540 of 11285 results

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pcDNA5FRTTO-codoptUL33-CStHA

Plasmid

#135106

Purpose

Codon-optimized HSV-1 UL33 with C-terminal StrepHA tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 UL33 codon optimized

Use

Tags

Strep-Strep-HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRTTO-codoptUS2-CStHA

Plasmid

#135107

Purpose

Codon-optimized HSV-1 US2 with C-terminal StrepHA tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 US2 codon optimized

Use

Tags

Strep-Strep-HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ Flag-RAP2A

Plasmid

#128099

Purpose

Expresses RAP2A in mammalian cells in a G418-selectable plasmid

Depositor

Chi Van Dang

Article

Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

Insert

RAP2A (RAP2A Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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UL91-2xStrep pCDNA4

Plasmid

#120375

Purpose

Expresses HCMV UL91with Cterm 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

UL91 (UL91 Human Cytomegalovirus (HHV-5))

Use

Tags

2xStrep Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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miR-722 pCDNA

Plasmid

#97162

Purpose

luciferase assay

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

miR-722 (mir722 Zebrafish)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-IL-2signal-6xMyc

Plasmid

#128033

Purpose

Mammalian expression vector with N terminal IL-2 signal peptide and 6xMyc tag

Depositor

Oskar Laur

Type

Empty backbone

Use

Tags

6xMyc and IL-2 signal peptide

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) eGFP SH3BP5 G4

Plasmid

#129022

Purpose

Expresses an eGFP mRNA with the SH3BP5 G-quadruplex (G4) region in the 3' UTR

Depositor

Jeremy Wilusz

Article

Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

Insert

eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-Intu

Plasmid

#128869

Purpose

For mammalian expression of FLAG-tagged mouse Inturned

Depositor

Aimin Liu

Insert

Inturned (Intu Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3 mSmoothened NQ4

Plasmid

#126407

Purpose

Expression of Smoothened NQ4 in mammalian cell lines.

Depositor

Stacey Ogden

Article

Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

Insert

m-Smoothened (Smo Mouse)

Use

Tags

Expression

Mammalian

Mutation

Smo glycosylation site NQ4

Promoter

Available since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3 mSmoothened NQ3

Plasmid

#126406

Purpose

Expression of Smoothened NQ3 in mammalian cell lines.

Depositor

Stacey Ogden

Article

Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

Insert

m-Smoothened (Smo Mouse)

Use

Tags

Expression

Mammalian

Mutation

Smo glycosylation site NQ3

Promoter

Available since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPCSK3 S368A

Plasmid

#122680

Purpose

Expresses mutated mouse PCSK3 in mammalian cells (serine at position 368 mutated to alanine via site directed mutagenesis)

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)

Use

Tags

Expression

Mammalian

Mutation

changed Serine 368 to Alanine

Promoter

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-mTRAF3ip3∆TM-CFP

Plasmid

#125182

Purpose

Expresses mouse TRAF3IP3 ∆TM in mammalian cells

Depositor

Shao-Cong Sun

Insert

TRAF3ip3 (Traf3ip3 Mouse)

Use

Tags

CFP

Expression

Mammalian

Mutation

K87R and S359P- See depositor comments below

Promoter

CMV

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-PU.1-ETS N236H

Plasmid

#124616

Purpose

Chimeric ETS domain: N236H

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (ETS1, SPI1 Human)

Use

Tags

Expression

Mammalian

Mutation

Residues 236 altered from asparagine to a histidi…

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-PU.1-ETS N236Q

Plasmid

#124615

Purpose

Chimeric ETS domain: N236Q

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Residues 236 altered from asparagine to a glutami…

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-PU.1-ETS N236Y

Plasmid

#124614

Purpose

Chimeric ETS domain: N236Y

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Residues 236 altered from asparagine to a Tyrosine

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (-) hYAP 1-1beta

Plasmid

#124140

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Tags

Expression

Mutation

None (Wild Type)

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RPS14(2-88)

Plasmid

#122298

Purpose

expresses a part of RPS14 protein with a flag tag

Depositor

Gerardo Ferbeyre

Article

Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

Insert

RPS14 (partial) (RPS14 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 89 to 151

Promoter

CMV

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-Y233C-HA

Plasmid

#120904

Purpose

Expresses Ptch1-B (Y233C) for function assays

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291, Y…

Promoter

CMV

Available since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-L234C-HA

Plasmid

#120905

Purpose

Expresses Ptch1-B (L234C)

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291, L…

Promoter

CMV

Available since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-HF2-BE3

Plasmid

#120397

Purpose

Expresses HF2-BE3 in mammalian cells

Depositor

Junjiu Huang

Article

Liang et al Protein Cell. 2017 Aug;8(8):601-611. doi

Insert

HF2-BE3

Use

Tags

Expression

Mammalian

Mutation

Mammalian codon-optimized Cas9-HF

Promoter

CMV

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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