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We narrowed to 13,582 results for: pET

Showing: 3521 - 3540 of 13582 results

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pET28a-MGGG-ybbr-HIS-SdrD_B1-DK

Plasmid

#117983

Purpose

SdrD B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB

Depositor

Hermann Gaub

Article

Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

Insert

SdrG_B2

Use

Tags

HIS, sortase, and ybbr

Expression

Bacterial

Mutation

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

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pET His10-Draper delta ITAM

Plasmid

#126671

Purpose

Expression of His10-Draper delta ITAM mutant for purification from E. coli

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper cytoplasmic ITAM residues mutated to Phe

Use

Tags

Expression

Bacterial

Mutation

Cytoplasmic ITAM residues mutated to Phe

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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Kv7.4 BD (522-645, C643A)/pET28HMT

Plasmid

#111073

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Use

Tags

His Tag and MBP tag

Expression

Bacterial

Mutation

Kv7.4 BD (522-645, C643A)

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

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pET264-pUC 24xMS2V6 Loxp KANr Loxp

Plasmid

#104393

Purpose

Yeast endogenous mRNA tagging

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V6

Use

Tags

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Promoter

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pET His6 TEV LIC cloning vector (1B)

Plasmid

#29653

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pET28a-6xHis-MBP-TEV site-TadA8e

Plasmid

#208319

Purpose

Overxpression MBP-TadA8e

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8e

Use

Tags

Expression

Bacterial

Mutation

mutations in TadA are described in the paper

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-6xHis-MBP-TEV site-TadA8r

Plasmid

#208318

Purpose

Overxpression MBP-TadA8r

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r

Use

Tags

Expression

Bacterial

Mutation

mutations in TadA are described in the paper

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pET251-pUC 12xMS2V6 Loxp KANr Loxp

Plasmid

#104392

Purpose

Yeast endogenous mRNA tagging

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

12xMS2V6

Use

Tags

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Promoter

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-6xHis-MBP-TEV site-TadA8.20

Plasmid

#194702

Purpose

Expresses N-terminal MBP fused TadA8.20 in bacteria

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

TadA8.20

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

mutations in TadA are described in the paper

Promoter

T7 promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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VR124 pETv2 10His-pG-Tn5 (E54K, L372P)

Plasmid

#198468

Purpose

E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.

Depositor

Viviana Risca

Article

Soroczynski et al bioRxiv 2024.07.11.602973

Insert

10His-pG-Tn5 (E54K, L372P)

Use

Tags

10His-pG

Expression

Bacterial

Mutation

E54K, L372P

Promoter

Consensus φ10 T7 Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP

Plasmid

#104394

Purpose

Yeast MCP-GFP expression

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

MCP-NLS-2xyeGFP

Use

Tags

Expression

Bacterial and Yeast

Mutation

MCP fused to NLS(SV40) and 2x yeast codon optimiz…

Promoter

CYC1

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pET His6 TEV LIC cloning vector (2B-T)

Plasmid

#29666

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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VR122 pETv2 10His-pG-Tn5 (E54K, L372P)

Plasmid

#198467

Purpose

E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in DH5α cloning strain.

Depositor

Viviana Risca

Article

Soroczynski et al bioRxiv 2024.07.11.602973

Insert

10His-pG-Tn5 (E54K, L372P)

Use

Tags

10His-pG

Expression

Bacterial

Mutation

E54K, L372P

Promoter

Consensus φ10 T7 Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET mCherry LIC cloning vector (u-mCherry)

Plasmid

#29769

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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p36-p37-p38-p40-pET-Duet-1

Plasmid

#175049

Purpose

Overexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coli

Depositor

Brian Kelch

Article

Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

Replication factor C subunits 2-5 (RFC4, RFC2, RFC3, RFC5 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pET21b(+)_SARS-CoV-2_3CLpro-C145A-Q306A

Plasmid

#177335

Purpose

bacterial expression of inactive SARS-CoV-2 3C-like-C145A proteinase

Depositor

Christopher Overall

Article

Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:

Insert

SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)

Use

Tags

Factor Xa site-3xFlag-Myc-6xHis

Expression

Bacterial

Mutation

silent mutation C to T at position 10546 (elimina…

Promoter

T7

Available since

Nov. 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a-GGG-HIS-CBM-Xmod-DocIII

Plasmid

#91698

Purpose

CBM-Xmod-DocIII handle with N-Terminal Sortase Recognition Sequence GGG for posttranslational coupling to a C-Terminal LPETGG

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

GGG-HIS-CBM-Xmod-DocIII

Use

Tags

HIS-Tag and Sortase-Tag

Expression

Bacterial

Mutation

Cysteine deletion in CBM C63S

Promoter

T7

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pET His6 LIC cloning vector (2Bc-T)

Plasmid

#37236

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pETM6-G6-vioABE-4A6-vioC-3A2-vioD

Plasmid

#73440

Purpose

Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.

Depositor

Mattheos Koffas

Article

Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

Insert

PG6-vioABE-P4A6-vioC-P3A2-vioD

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PG6, P4A6, and P3A2 (orthogonal T7-lac variants)

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pET mOrange LIC cloning vector (u-mOrange)

Plasmid

#29770

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

mOrange

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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