-
Plasmid#80891Purposemammalian expression of Smad4 R100TDepositorInsertSmad4 (SMAD4 Human)
UseTagsFlagExpressionMammalianMutationPancreatic cancer mutation R100TPromoterCMVAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pS2-FLAG-Sestrin2-E451A
Plasmid#73679PurposeoverexpressionDepositorInsertSesn2 (SESN2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationE451A changed Glutamate 451 to AlaninePromoterSesn2Available sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
3XFLAG-NSarQ27G-VMAn-FKBP
Plasmid#70229PurposeExpresses FLAG tagged N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicingDepositorInsertalpha Sarcin (N-terminal portion)
UseTags3XFLAG, FK506- and rapamycin-binding protein (FKB…ExpressionMammalianMutationChanged Gln 27 to GlyPromoterCMVAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag BimL (S58D)
Plasmid#24239DepositorInsertBimL (S58D) (BCL2L11 Human)
UseTagsFlagExpressionMammalianMutationChanged serine 58 to aspartic acidPromoterAvailable sinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRK-FLAG-TRAF4(K190R)
Plasmid#58317PurposeFLAG-tagged mouse TRAF4 K190R mutantDepositorInsertTNF receptor associated factor 4 (Traf4 Mouse)
UseTagsFLAGExpressionMammalianMutationK 190 to RPromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Flag RagB (CTD)
Plasmid#112769Purposeexpress Flag-RagB (C terminal domain only)DepositorInsertFlag-RagB (C terminal domain only) (RRAGB Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Flag RagC (CTD)
Plasmid#112771Purposeexpress Flag-RagC (C terminal domain only)DepositorInsertFlag-RagC (C terminal domain only) (RRAGC Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG-KIF17-short-pcDNA3
Plasmid#47545PurposeExpresses FLAG epitope-tagged short form of mouse KIF17 in mammalian cellsDepositorInsertKIF17-short (Kif17 Mouse)
UseTagsFLAG epitopeExpressionMammalianMutationG152S*PromoterCMVAvailable sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
FLAG-PPP2R5E siRNA-resistant
Plasmid#120861Purposeexpresses dox-inducible PPP2R5E resistant to siRNA pool 2 used in manuscriptDepositorInsertPPP2R5E (PPP2R5E Human)
UseTagsFLAGExpressionMammalianMutationsilent mutation at aa 282-284 in PPP2RE to genera…PromoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCin4-3xFLAG-MKL1 L272A
Plasmid#19850DepositorInsertMKL1 L272A (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationchanged Leucine 272 to AlaninePromoterAvailable sinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NmCas9-Flag H588A
Plasmid#109032PurposeH588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
UseTagsFlag TagExpressionBacterialMutationPromoterT7Available sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag BimL (S44D)
Plasmid#24235DepositorInsertBimL (S44D) (BCL2L11 Human)
UseTagsFlagExpressionMammalianMutationChanged serine 44 to aspartic acidPromoterAvailable sinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag-YAP-P85A
Plasmid#33062DepositorInsertYAP (YAP1 Human)
UseTagsFlagExpressionMammalianMutationP85APromoterAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Tipin-L195A
Plasmid#23116DepositorInsertTipin (TIPIN Human)
UseTagsFlagExpressionMammalianMutationchanged Leucine 195 to AlaninePromoterAvailable sinceFeb. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCin4-3xFLAG-MKL1 S449A
Plasmid#19842DepositorInsertMKL1 S449A (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationchanged Serine 449 to AlaninePromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
V25 pLP Flag - Acceptor
Plasmid#11703DepositorTypeEmpty backboneUseCre/Lox; Acceptor vectorTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 26, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pfast bac-flag Fbxl3
Plasmid#51894PurposeBackbone plasmid is pfastBacHTa from invitrogenDepositorInsertFbxl3 (Fbxl21 Mouse)
UseTagsFLAGExpressionMutationPromoterpolyhedrin promoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 PARL-FLAG-CT S70A
Plasmid#13620DepositorUseTagsFLAGExpressionMammalianMutationchanged Serine 70 to AlaPromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
flag-hKLF6 P149S (1008)
Plasmid#49496Purposeexpresses human Flag tagged KLF6 with P149S mutationDepositorInsertKFL6-P149S (KLF6 Human)
UseTagsFlagExpressionMammalianMutationP149SPromoterCMVAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG/MKRN1(1-280)
Plasmid#78752PurposeTo overexpress MKRN1(1-280) in Mammalian CellsDepositorInsertMKRN1 (MKRN1 Human)
UseTagsFLAGExpressionMammalianMutation1-280PromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only