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Plasmid#227016PurposeVector encoding human codon-optimized enhanced-activity OgeuIscB fusion with extra SV40 NLS driven by CMV promoterDepositorInsertSV40 NLS-OgeuIscB-v3-SV40-nucleoplasmin NLS
UseTagsExpressionMutationD96R/E84R/V159RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NRCH-ABE8.17-m+V106W
Plasmid#198551PurposeExpresses human codon-optimized SpCas9-NRCH-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8.17-m+V106W-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRCH-ABE8.17-m+V106W
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NRCH-APOBEC-nCas9-Ung
Plasmid#198552PurposeExpresses human codon-optimized SpCas9-NRCH-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NRCH-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRCH-APOBEC-nCas9-Ung
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG-ABE8.17-m+V106W
Plasmid#198534PurposeExpresses human codon-optimized SpCas9-NG-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8.17-m+V106W-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG-ABE8.17-m+V106W
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG-APOBEC-nCas9-Ung
Plasmid#198539PurposeExpresses human codon-optimized SpCas9-NG-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NG-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG-APOBEC-nCas9-Ung
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1 human full-length CENP-E-mNeon-6xHis
Plasmid#180484PurposepFastBac1 human CENP-E-mNeon-His, containing a 3c protease cleavage site between mNeon and His tag. Codon optimized for expression in Sf9 cellsDepositorInsertCENP-E (CENPE Synthetic, Human, human)
UseTagsmNeon-6xHisExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
PD2529 human beta1 full
Plasmid#221401PurposeMammalian expression of human integrin beta1 full-lengthDepositorInsertintegrin beta1 full-length (ITGB1 Human)
UseTagsCD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …ExpressionMammalianMutationcodon-optimized mature sequencePromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IRES-blast GFP-SPNS1
Plasmid#198571PurposeSPNS1 codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vectorDepositorInsertSPNS1 with n-terminal 3xFlag-GFP fusion (SPNS1 Human)
UseRetroviralTags3xFlag-GFPExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXs-3xFlag-SPNS1
Plasmid#198568PurposeSPNS1 codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vectorDepositorInsertSPNS1 with n-terminal 3xFlag (SPNS1 Human)
UseRetroviralTags3xFlagExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1
Plasmid#216378PurposeBaculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)DepositorInsertFrizzled-4 (Fzd4 Mouse)
UseBaculovirusTagsHA signal sequence-FLAGExpressionInsectMutationPromoterPolyhedrinAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP4-EGFP
Plasmid#210378PurposeExpresses SARS-CoV-2 NSP4 fused with EGFP in mammalian cellsDepositorInsertNSP4 (ORF1ab SARS-CoV-2)
UseTagsEGFPExpressionMammalianMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP2-Flag
Plasmid#210344PurposeExpresses SARS-CoV-2 NSP2 in mammalian cellsDepositorInsertSARS-CoV-2 NSP2 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
UseTagsFlagExpressionMammalianMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP3C-mCherry
Plasmid#210377PurposeExpresses the C terminal of SARS-CoV-2 NSP3 fused with mCherry tag in mammalian cellsDepositorInsertNSP3C (ORF1ab SARS-CoV-2)
UseTagsmCherryExpressionMammalianMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP4-FR-2Strep
Plasmid#210316PurposeExpresses SARS-CoV-2 NSP4 in mammalian cellsDepositorInsertSARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
UseTags2StrepExpressionMammalianMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
(B) G418 marker (R) (SBE 137)
Plasmid#195011Purposegeneticin (G418) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tNOSDepositorInsertpXYL+G418+tNOS
UseTagsExpressionBacterialMutationPromoterpXYLAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
p202-Syt1-C2A-I248V
Plasmid#201554PurposeExpresses Octopus synaptotagmin-1 C2A domain with I248V mutation in E. coliDepositorInsertSynaptotagmin-1 C2A domain (partial)
UseTagsExpressionBacterialMutationchanged Isoleucine 248 to Valine (numbering based…PromoterRBSAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-V5-HisB-ObKHC-K282R-HaloTag
Plasmid#201552PurposeExpresses Octopus kinesin-1 motor with K282R mutation with HaloTag in Drosophila S2 cellsDepositorInsertKinesin-1
UseTagsHaloTagExpressionInsectMutationchanged lysine 282 to arginine (numbering based o…PromoterAc5Available sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
B-HeFSpCas9-A695Q
Plasmid#207368PurposeExpression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variantDepositorInsertB-HeFSpCas9-A695Q
UseCRISPRTags3xFLAGExpressionMammalianMutationN497A, R661A, K848A, Q926A, K1003A, R1060A, amino…PromoterCBhAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
B-HeFSpCas9-A926Q
Plasmid#207370PurposeExpression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variantDepositorInsertB-HeFSpCas9-A926Q
UseCRISPRTags3xFLAGExpressionMammalianMutationN497A, R661A, Q695A, K848A, K1003A, R1060A, amino…PromoterCBhAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
B-evoSpCas9-E526K
Plasmid#207365PurposeExpression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variantDepositorInsertB-evoSpCas9-E526K
UseCRISPRTags3xFLAGExpressionMammalianMutationM495V, Y515N, R661Q, amino acids 1005-1013 replac…PromoterCBhAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only