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Plasmid#208628Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa1~41, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 1~41 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 1~41, other residues not includedPromoterT7Available sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_His-CBD-EmEndoF3
Plasmid#218194PurposeBacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF3DepositorInsertEndoF3
UseTagstufA-His-CBDExpressionBacterialMutationcodon optimized Ecoli, residues 41-329PromoterT7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_His-CBD-EmEndoF1
Plasmid#218195PurposeBacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF1DepositorInsertEndoF1
UseTagstufA-His-CBDExpressionBacterialMutationcodon optimized Ecoli, residues 51-339PromoterT7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt1 (96-421) I367C
Plasmid#213620PurposeBacterial expression of SUMO-tagged rat syt1 (96-421) I367CDepositorInsertsyt1 (96-421) I367C (Syt1 Rat)
UseTagsSUMOExpressionBacterialMutationC277A; I367CPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 (136-403)
Plasmid#213621PurposeBacterial expression of SUMO-tagged mouse syt7 (136-403)DepositorInsertsyt7 (136-403) (Syt7 Mouse)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-Doc2β C2AB (G361C)
Plasmid#213631PurposeBacterial expression of SUMO-tagged rat Doc2β C2AB (G361C)DepositorInsertDoc2β C2AB (G361C) (Doc2b Rat)
UseTagsSUMOExpressionBacterialMutationG361C, all original cysteines mutated to alaninesPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-cPLA2 C2 domain (Y96C)
Plasmid#213633PurposeBacterial expression of SUMO-tagged human cPLA2 C2 domain (Y96C)DepositorInsertcPLA2 C2 domain (Y96C) (PLA2G4A Human)
UseTagsSUMOExpressionBacterialMutationY96C, C139A, C141SPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_mouse-Pro-IL-18_humanized-triple-mutant
Plasmid#214339PurposeProtein overexpression in bacteriaDepositorInsertIL18 (Il18 Mouse)
UseTagsHis-TEVExpressionBacterialMutationENGD29DDEN_N36Y_H41EPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-His6-CUbo(K63R)-MCS
Plasmid#212727PurposeBacterial expression of a protein of interest with N-terminal His6-CUbo(K63R)-fusion, contains multiple cloning siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-His6-CUbo(K48R)-MCS
Plasmid#212726PurposeBacterial expression of a protein of interest with N-terminal His6-CUbo(K48R)-fusion, contains multiple cloning siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15b-His6-CUbo(K63R)-GFP
Plasmid#212722PurposeBacterial expression of His6-CUbo(K63R)-GFPDepositorInsertGFP
UseTags6xHis-CUbo(K63R)ExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15b-His6-CUbo(K48R)-GFP
Plasmid#212721PurposeBacterial expression of His6-CUbo(K48R)-GFPDepositorInsertGFP
UseTags6xHis-CUbo(K48R)ExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-Δ1~34,Δ215~284-TwStrep
Plasmid#208630Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa35~398 with deletion of 215~284, fused with Twin Strep tag.DepositorInserthuman RPL3 (ENST00000216146) Δ1~34, Δ215~284 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationΔ1~34,Δ215~284PromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-53~77-mEGFP(A206K)-TwStrep
Plasmid#208632Purposefor E. coli expression truncated human RPL3 (ENST00000216146), aa53~77, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 53~77 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 53~77, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-1~77-mEGFP(A206K)-TwStrep
Plasmid#208624Purposefor E. coli expression truncated human RPL3 (ENST00000216146), aa1~77, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 1~77 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 1~77, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7RE ∆238-245
Plasmid#139318PurposeGST tagged AO7 encoding aa 126-258 with an internal deletion that results in loss of E2 binding for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
UseTagsGSTExpressionBacterialMutationAO7 cloned into the first multiple cloning site o…PromoterT7 promoter-1Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET16b-RPA4/His14 (MSW#949)
Plasmid#208075PurposeExpression of human Replication Protein A 4 and His-tagged RPA3 in E.coliDepositorInsertRPA4, RPA14(His-tagged)
UseTags10xHis tag on RPA14ExpressionBacterialMutationPromoterAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b+His SUMO Δ37 PPM1H
Plasmid#207334PurposeBacterial expression of His SUMO Δ37 PPM1HDepositorInsertPPM1H (PPM1H Human)
UseTagsHis-SUMOExpressionBacterialMutationΔ37PromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b+His SUMO Rab10(Q68L)
Plasmid#207342PurposeBacterial expression of His SUMO Rab10(Q68L)DepositorInsertRab10 (RAB10 Human)
UseTagsHis-SUMOExpressionBacterialMutationQ68LPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-B-HypaR-SpCas9-NLS-6xHis
Plasmid#207388PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-HypaR-SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationR661A, N692A, M694A, Q695A, H698A, amino acids 10…PromoterT7Available sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only