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We narrowed to 13,582 results for: pET

Showing: 3501 - 3520 of 13582 results

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pET151-hUCH37 (ISF1)(M148A/F149A)

Plasmid

#61933

Purpose

Expression of hUCH37 (M148A/F149A) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

hUCH37 (ISF1) (UCHL5 Human)

Use

Tags

6x HIS

Expression

Bacterial

Mutation

Methionine 148 and Phenylalanine 149 were mutated…

Promoter

T7

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pETDUET1-hUCH37 (ISF1) - hNFRKB (1-117)

Plasmid

#61941

Purpose

Co-expression of hUCH37 (ISF1) and hNFRKB (1-117) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Inserts

hUCH37 (ISF1) (UCHL5 Human)
hNFRKB (1-117) (NFRKB Human)

Use

Tags

6x HIS

Expression

Bacterial

Mutation

Construct ends at residue 117, stop codon introdu…

Promoter

T7

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
213-petDuet1-GBD-LUMP--cdc42

Plasmid

#65973

Purpose

Dual expression of GBD-LUMP and cdc42

Depositor

Gerard Marriott

Article

Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

Inserts

LUMP
cell division cycle 42 (CDC42 Human)

Use

Tags

GBD (GTPase binding domain from ACK1; residues 44…

Expression

Bacterial

Mutation

constitutively active Cdc42 (Q61L)

Promoter

T7

Available since

July 13, 2015

Availability

Academic Institutions and Nonprofits only

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pET11+PCNA3-CYP119(D77R/T214V)

Plasmid

#66138

Purpose

Expresses the D77R/T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Use

Tags

Sulfolobus solfataricus PCNA3

Expression

Bacterial

Mutation

D77R, T214V

Promoter

T7 promoter

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pET-22HT-MSI1(7-192)Q30D

Plasmid

#60346

Purpose

Q30D mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Use

Tags

His and TEV protease site

Expression

Bacterial

Mutation

Q30D; contains amino acids 7-192 of Msi1

Promoter

T7

Available since

Dec. 1, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)R53E

Plasmid

#60352

Purpose

R53E mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Use

Tags

His and TEV protease site

Expression

Bacterial

Mutation

R53E; contains amino acids 7-192 of Msi1

Promoter

T7

Available since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)G64A

Plasmid

#60351

Purpose

G64A mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Use

Tags

His and TEV protease site

Expression

Bacterial

Mutation

G64A; contains amino acids 7-192 of Msi1

Promoter

T7

Available since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)H83L

Plasmid

#60350

Purpose

H83L mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Use

Tags

His and TEV protease site

Expression

Bacterial

Mutation

H83L; contains amino acids 7-192 of Msi1

Promoter

T7

Available since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)G35D

Plasmid

#60347

Purpose

G35D mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Use

Tags

His and TEV protease site

Expression

Bacterial

Mutation

G35D; contains amino acids 7-192 of Msi1

Promoter

T7

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)G35E

Plasmid

#60348

Purpose

G35E mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Use

Tags

His and TEV protease site

Expression

Bacterial

Mutation

G35E; contains amino acids 7-192 of Msi1

Promoter

T7

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)H83F

Plasmid

#60349

Purpose

H83F mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Use

Tags

His and TEV protease site

Expression

Bacterial

Mutation

H83F; contains amino acids 7-192 of Msi1

Promoter

T7

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pET28a-ybbR-HIS-Spec(x2)-DocI

Plasmid

#58713

Purpose

Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

Depositor

Hermann Gaub

Article

Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

Insert

Spec(x2)-DocI

Use

Tags

HIS, HRV 3C, and ybbR

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pET15b/GgVcl 1-851 A50I mutation

Plasmid

#46174

Depositor

Susan Craig

Article

Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

Insert

Vcl 1-851 A50I (VCL Chicken)

Use

Tags

His

Expression

Bacterial

Mutation

aa 1-851 A50I mutation (talin binding domain mut…

Promoter

T7

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pET15b/GgVcl 1-258 A50I mutation

Plasmid

#46175

Depositor

Susan Craig

Article

Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

Insert

Vcl 1-258 A50I (VCL Chicken)

Use

Tags

His

Expression

Bacterial

Mutation

aa 1-258 (VD1) A50I mutation; talin binding defic…

Promoter

T7

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pET28b(+)_S. cerevisiae Hbs1(162-611)

Plasmid

#39337

Depositor

SeWon Suh

Insert

Elongation factor 1 alpha-like protein

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pET-21a(+)-DR RecR(K23A/R27A)

Plasmid

#12619

Depositor

SeWon Suh

Article

Lee et al EMBO J. 2004 May 19. 23(10):2029-38.

Insert

Deinococcus radiodurans RecR (recR Deinococcus radiodurans)

Use

Tags

His

Expression

Bacterial

Mutation

changed Lysine 23 to Alanine, and Arginine 27 to …

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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p141 pETF-GST RanBP10 G308R

Plasmid

#11094

Depositor

Ramesh Shivdasani

Insert

RanBP10 (Ranbp10 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

G308R

Promoter

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only

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Pet28a His6-HSPB7 (zebrafish) C117S

Plasmid

#110072

Purpose

Express C117S mutant of Zebrafish His6-HSPB7 in bacteria

Depositor

Yimon Aye

Article

Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

Insert

His6-HSPB7 (hspb7 Zebrafish)

Use

Tags

His6

Expression

Bacterial

Mutation

Cysteine 117 to Serine

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

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pET28a-MGGG-ybbr-HIS-SdrG_B1-DK

Plasmid

#117981

Purpose

SdrG B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB

Depositor

Hermann Gaub

Article

Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

Insert

SdrG_B1

Use

Tags

HIS, sortase, and ybbr

Expression

Bacterial

Mutation

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

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pET28a-MGGG-ybbr-HIS-SdrG_B2-DK

Plasmid

#117982

Purpose

SdrG B2 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB

Depositor

Hermann Gaub

Article

Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

Insert

SdrG_B2

Use

Tags

HIS, sortase, and ybbr

Expression

Bacterial

Mutation

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

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