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Plasmid#158511PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1-6xHA. Km plant selection marker.DepositorInsertZAR1-6xHA (ZAR1 Mustard Weed)
UseTags6xHAExpressionPlantMutationPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA20
Plasmid#158498PurposeIn planta gene expression of Golden gate complatible A. thaliana ZAR1 D498V/L17E. Km plant selection marker.DepositorInsertZAR1 (ZAR1 Mustard Weed)
UseTagsExpressionPlantMutationL17E, D489VPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA26
Plasmid#158504PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V-4xMyc. Km plant selection marker.DepositorInsertZAR1-4xMyc (ZAR1 Mustard Weed)
UseTags4xMycExpressionPlantMutationD489VPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA41
Plasmid#158519PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1-eGFP. Km plant selection marker.DepositorInsertZAR1-eGFP (ZAR1 Mustard Weed)
UseTagseGFPExpressionPlantMutationPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA43
Plasmid#158521PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 L17E-eGFP. Km plant selection marker.DepositorInsertZAR1-eGFP (ZAR1 Mustard Weed)
UseTagseGFPExpressionPlantMutationL17EPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA42
Plasmid#158520PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V-eGFP. Km plant selection marker.DepositorInsertZAR1-eGFP (ZAR1 Mustard Weed)
UseTagseGFPExpressionPlantMutationD489VPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA34
Plasmid#158512PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V-6xHA. Km plant selection marker.DepositorInsertZAR1-6xHA (ZAR1 Mustard Weed)
UseTags6xHAExpressionPlantMutationD489VPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA28
Plasmid#158506PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V/L17E-4xMyc. Km plant selection marker.DepositorInsertZAR1-4xMyc (ZAR1 Mustard Weed)
UseTags4xMycExpressionPlantMutationL17E, D489VPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA25
Plasmid#158503PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1-4xMyc. Km plant selection marker.DepositorInsertZAR1-4xMyc (ZAR1 Mustard Weed)
UseTags4xMycExpressionPlantMutationPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS424-ysg(G:H)
Plasmid#138257PurposeExpression of GFP-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequence to be used in yeast dual reporter systemDepositorInsertGFP targeting sgRNAs G and H
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-hsg(G:H)
Plasmid#138259PurposeExpression of humanized mCherry-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequences to be used in Traffic Light reporter systemDepositorInsertmCherry targeting sgRNAs G and H
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCB6-HAOccludin
Plasmid#136638PurposeExpression of HAOccludin in mammalian cellsDepositorInsertHAOccludin (OCLN Chicken)
UseTagsHA (MQDLPGNDNSTAG)ExpressionMammalianMutationPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCB6-HAOccludinCT3
Plasmid#136640PurposeExpression of HAOccludinCT3 in mammalian cellsDepositorInsertHAOccludinCT3 (OCLN Chicken)
UseTagsHA (MQDLPGNDNSTAG)ExpressionMammalianMutationDeletion of C-terminal cytoplasid domainPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
ORF18(R158A_L159A)-3xFLAG pCDNA4
Plasmid#129814PurposeExpresses KSHV ORF18 (R158A_L159A) with Cterm 3xFLAG tag in mammalian cellsDepositorInsertORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
UseTags3xFLAGExpressionMammalianMutationchanged Arginine 158 to Alanine and Leucine 159 t…PromoterCMVAvailable sinceOct. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
ORF18(G228A)-3xFLAG pCDNA4
Plasmid#129809PurposeExpresses KSHV ORF18 (G228A) with Cterm 3xFLAG tag in mammalian cellsDepositorInsertORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
UseTags3xFLAGExpressionMammalianMutationchanged Glycine 228 to AlaninePromoterCMVAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
ORF18(L29A)-3xFLAG pCDNA4
Plasmid#129777PurposeExpresses KSHV ORF18 (L29A) with Cterm 3xFLAG tag in mammalian cellsDepositorInsertORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
UseTags3xFLAGExpressionMammalianMutationchanged Leucine 29 to AlaninePromoterCMVAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
ORF18(L37A)-3xFLAG pCDNA4
Plasmid#129779PurposeExpresses KSVH ORF18 (L37A) with Cterm 3xFLAG tag in mammalian cellsDepositorInsertORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
UseTags3xFLAGExpressionMammalianMutationchanged Leucine 37 to AlaninePromoterCMVAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
ORF18(K75A)-3xFLAG pCDNA4
Plasmid#129784PurposeExpresses KSHV ORF18 (K75A) with Cterm 3xFLAG tag in mammalian cellsDepositorInsertORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
UseTags3xFLAGExpressionMammalianMutationchanged Lysine 75 to AlaninePromoterCMVAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
ORF18(G65A)-3xFLAG pCDNA4
Plasmid#129781PurposeExpresses KSHV ORF18 (G65A) with Cterm 3xFLAG tag in mammalian cellsDepositorInsertORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
UseTags3xFLAGExpressionMammalianMutationchanged Glycine 65 to AlaninePromoterCMVAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
ORF18(L107A)-3xFLAG pCDNA4
Plasmid#129787PurposeExpresses KSHV ORF18 (L107A) with Cterm 3xFLAG tag in mammalian cellsDepositorInsertORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
UseTags3xFLAGExpressionMammalianMutationchanged Leucine 107 to AlaninePromoterCMVAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only