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Plasmid#61418PurposeExpresses human OTUD6B (full-length) in E. coli.DepositorInsertOTUD6B (OTUD6B Human)
UseTagsHis6-GST-3CExpressionBacterialMutationPromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin
Plasmid#99278PurposeDual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistanceDepositorInsertsH2B-mVenus-IRES-mCherry
Puro
UseLentiviralTagsH2B, IRES, PGK, mCherry, and mVenusExpressionMutationPromoterEF1 alpha-human and PGK-mouseAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRepair-mScI-CTNNB1
Plasmid#153431PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mScarlet-iDepositorInsertCTNNB1 homology arms and mScI coding sequence (CTNNB1 Human)
UseCRISPR; Hdr donor templateTagsmScarlet-iExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SARS-CoV2-NP-Nt(47-173)
Plasmid#165091Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal(47-173) (N SARS-CoV2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
AttB_ACE2[dEcto]_IRES-mCherry-H2A-P2A-PuroR
Plasmid#171596PurposeEctodomain deleted negative control ACE2 from Bxb1 landing padDepositorInsertsUseSynthetic Biology; Promoterless bxb1 dna recombin…TagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR-53BP1 1-1972 WT
Plasmid#53446PurposeRecombinational donor/master vectorDepositorInsert53BP1 (TP53BP1 Human)
UseGatewayTagsExpressionMutationsiRNA resistant and mutations A128V, T720A, A1347…PromoterAvailable sinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRepair-SGFP2-CTNNB1
Plasmid#153430PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SGFP2DepositorInsertCTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)
UseCRISPR; Hdr donor templateTagsSGFP2ExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD5 (OTU, aa 171-358)
Plasmid#61415PurposeExpresses human OTUD5 (OTU domain) in E. coli.DepositorInsertOTUD5 (OTUD5 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-170 and aa 359-571.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAP03-sp44/p21
Plasmid#120232PurposeCarries bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites that can be amplified with homology sequence for refactoring.DepositorInsertbidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites
UseSynthetic BiologyTagsExpressionMutationaac(3)IV (apramycin resistance gene)PromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX459-CTNNB1-S45
Plasmid#164587PurposeExpresses a gRNA that overlaps the S45 codon of human CTNNB1DepositorInsertCTNNB1 gRNA (CTNNB1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DHFR.cHSF1
Plasmid#134739PurposeLentiviral expression vector for constitutively active HSF1 fused to DHFR destabilized domainDepositorInsertDHFR.cHSF1 (HSF1 Human)
UseLentiviralTagsDHFRExpressionMammalianMutationDeletion of amino acids 186-202PromoterCMVAvailable sinceAug. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX459-CTNNB1-ATG
Plasmid#153429PurposeExpresses a gRNA that overlaps the startcodon of human CTNNB1DepositorInsertCTNNB1 gRNA (CTNNB1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
psicheck2 PTEN 3'UTR
Plasmid#50936Purposereporter for PTEN RNAiDepositorInsertPTEN 3'UTR (PTEN Human)
UseRNAiTagsRenilla luciferaseExpressionMammalianMutationPromoterT7Available sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
PLX_307 SLC31A1
Plasmid#184491PurposeLentivirus expression for SLC31A1 geneDepositorInsertSLC31A1 (SLC31A1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCI-Neo-PTPRT (WT)
Plasmid#16630DepositorInsertPTPRT (PTPRT Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
p3s-ABE7.10
Plasmid#113128Purposeexpressing ABE7.10 (TadA-TadA*7.10-nCas9-NLS-HA), human codon optimizedDepositorInsertABE 7.10
UseTagsHA epitope and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET48 AtGUN1-SMR1
Plasmid#136361PurposeBacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 709 to 918)DepositorInsertcDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 709 to 918 (GUN1 Mustard Weed)
UseTagsTRX-HisExpressionBacterialMutationPromoterT7Available sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET48 AtGUN1-SMR2
Plasmid#136362PurposeBacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 781 to 918)DepositorInsertcDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 781 to 918 (GUN1 Mustard Weed)
UseTagsTRX-HisExpressionBacterialMutationPromoterT7Available sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET48 AtGUN1-PPR1
Plasmid#136359PurposeBacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 708)DepositorInsertcDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 708 (GUN1 Mustard Weed)
UseTagsTRX-HisExpressionBacterialMutationPromoterT7Available sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET48 AtGUN1-PS
Plasmid#136358PurposeBacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 918)DepositorInsertcDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 918 (GUN1 Mustard Weed)
UseTagsTRX-HisExpressionBacterialMutationPromoterT7Available sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only