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Plasmid#125825PurposeExpresses GST-RPN13 (19-132) K97E in E.coliDepositorInsertRPN13 (ADRM1 Human)
UseTagsGSTExpressionBacterialMutationchanged lysine 97 to glutamatePromoterT7Available sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) K83A
Plasmid#125829PurposeExpresses GST-RPN13 (19-132) K83A in E.coliDepositorInsertRPN13 (ADRM1 Human)
UseTagsGSTExpressionBacterialMutationchanged lysine 83 to alaninePromoterT7Available sinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) K97A
Plasmid#125823PurposeExpresses GST-RPN13 (19-132) K97A in E.coliDepositorInsertRPN13 (ADRM1 Human)
UseTagsGSTExpressionBacterialMutationchanged lysine 97 to alaninePromoterAvailable sinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) K97D
Plasmid#125824PurposeExpresses GST-RPN13 (19-132) K97D in E.coliDepositorInsertRPN13 (ADRM1 Human)
UseTagsGSTExpressionBacterialMutationchanged lysine 97 to aspartatePromoterT7Available sinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) R104A
Plasmid#125826PurposeExpresses GST-RPN13 (19-132) R104A in E.coliDepositorInsertRPN13 (ADRM1 Human)
UseTagsGSTExpressionBacterialMutationchanged arginine 104 to alaninePromoterT7Available sinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) R104D
Plasmid#125827PurposeExpresses GST-RPN13 (19-132) R104D in E.coliDepositorInsertRPN13 (ADRM1 Human)
UseTagsGSTExpressionBacterialMutationchanged arginine 104 to aspartatePromoterT7Available sinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) R104E
Plasmid#125828PurposeExpresses GST-RPN13 (19-132) R104E in E.coliDepositorInsertRPN13 (ADRM1 Human)
UseTagsGSTExpressionBacterialMutationchanged arginine 104 to glutamatePromoterT7Available sinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
Pet28a His6-HSPB7 (zebrafish) C49S
Plasmid#110071PurposeExpress C49S mutant of Zebrafish His6-HSPB7 in bacteriaDepositorInsertHis6-HSPB7 (hspb7 Zebrafish)
UseTagsHis6ExpressionBacterialMutationCysteine 49 to SerinePromoterT7Available sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FFSARG-ddFLN4-HIS-ybbr
Plasmid#101240Purposeminimal SdrG binding peptide FFSARG with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forcesDepositorInsertFFSARG peptide, ddFLN4 fingerprint
UseTagsHIS and ybbrExpressionBacterialMutationC18S in ddFLN4PromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET11a asyn Q79N (TAC-->TAT)
Plasmid#105733PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein Q79N (TAC-->TAT) (SNCA Human)
UseTagsExpressionBacterialMutationQ79NPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pet151D-TOPO-BROX 1-411; K141E
Plasmid#89865PurposeExpresses full length human BROX with mutation K141E in bacteria; internal ID WISP11-299DepositorInsertBROX (BROX Human)
UseTagsHis Tag, V5 tagExpressionBacterialMutationmutated lysine 141 to glutamic acidPromoterT7Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pet151D-TOPO-BROX 1-411; L212D
Plasmid#89864PurposeExpresses full length human BROX with mutation L212D in bacteria; internal ID WISP11-300DepositorInsertBROX (BROX Human)
UseTagsHis Tag, V5 tagExpressionBacterialMutationmutated leucine 212 to aspartic acidPromoterT7Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (918-953)
Plasmid#73743PurposeExpression of GFP-PP-RPN2 (918-53) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
UseTags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 918-953 of RPN2PromoterT7Available sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (932-953)
Plasmid#73745PurposeExpression of GFP-PP-RPN2 (932-953) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
UseTags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 932-953 of RPN2PromoterT7Available sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (918-947)
Plasmid#73747PurposeExpression of GFP-PP-RPN2 (918-947) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
UseTags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 918-947 of RPN2PromoterT7Available sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (797-953)
Plasmid#74228Purpose[Edit] Expression of GFP-PP-RPN2 (797-953) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
UseTags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 918-953 of RPN2PromoterT7Available sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437) L151D
Plasmid#87736PurposeBacterial expression of a monomeric variant of the S. cerevisiae Vps4 AAA ATPase cassetteDepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
UseTags6xHis-V5-TEVExpressionBacterialMutationdeleted amino acids 1-100, mutated Leu 151 to AspPromoterT7Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 Q147A, M151A, I154A
Plasmid#74616PurposeExpresses a triple mutant truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-239, Q147A, M151A, I154A (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationQ147A, M151A, I154APromoterT7Available sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 N68A, W69A, K77A
Plasmid#74619PurposeExpresses a triple mutant truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-239, N68A, W69A, K77A (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET151-hUCH37 (ISF1)(M148D/F149D)
Plasmid#71388PurposeExpression of hUCH37 (M148D/F149D) in E.coliDepositorInserthUCH37 (ISF1) M148D/F149D (UCHL5 Human)
UseTagsHIS-TEV sequenceExpressionBacterialMutationchanged methionine 148 to aspartate, phenylalanin…PromoterT7Available sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only