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Showing: 3481 - 3500 of 35162 results

  1. pTK Cas9-2A-Citrine

    Plasmid
    #92393
    Purpose
    Enhancer/reporter plasmid for tissue-specific expression of Cas9 with 2A-Citrine reporter. Contains BsmBI-flanked LacZ cloning cassette for rapid GoldenGate-based cloning of specific enhancers.
    Depositor
    Tatjana Sauka-Spengler
    Article
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Insert
    Cas9 2A Citrine
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    thymidine kinase promoter
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    92393-plasmid-map-sequence-id-190866

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  2. TREML1-bio-His

    Plasmid
    #51857
    Purpose
    Expresses full-length Trem-like transcript 1 protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TREML1 (TREML1 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51857-plasmid-map-sequence-id-79745

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  3. TMED2-bio-His

    Plasmid
    #51853
    Purpose
    Expresses full-length Transmembrane emp24 domain-containing protein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TMED2 (TMED2 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51853-plasmid-map-sequence-id-79740

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  5. pMT-flk1-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80056
    Purpose
    Flk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    flk1
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80056-plasmid-map-sequence-id-151372

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  16. pPPC011

    Plasmid
    #171143
    Purpose
    Expression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmid
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Insert
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    Sp.pCas9, BBa_J23107
    Available since
    Oct. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    171143-plasmid-map-sequence-id-345216

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  18. TMED1-bio-His

    Plasmid
    #51832
    Purpose
    Expresses full-length Transmembrane emp24 domain-containing protein 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TMED1 (TMED1 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51832-plasmid-map-sequence-id-79669

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  20. pET14b-mEKLF

    Plasmid
    #67834
    Purpose
    Encodes murine EKLF (nucleotides 71-1215) for in vitro chromatin/transcription reconstitution
    Depositor
    James Bieker
    Article
    Armstrong et al Cell. 1998 Oct 2;95(1):93-104.
    Insert
    EKLF (Klf1 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Full-length mEKLF is aa 20-376; amino acid 19 is …
    Promoter
    T7
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 3481 - 3500 of 35162 results