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Plasmid#126424PurposeExpresses Cas9-T2A-TdT in mammalian cells. (pTBL209)DepositorInsertCas9-T2A-TdT (DNTT Streptococcus pyogenes, Human)
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-BATF
Plasmid#178451Purposedoxycycline-inducible expression of Human BATF in mammalian cellsDepositorInsertBATF (BATF Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSSRB070_pAC8-hsCRBN
Plasmid#218789PurposeInsect cell expression vector for FLAG-TEV-Spy-hsCRBNDepositorInsertProtein cereblon (CRBN Human)
UseTagsFlag-TEV-SpyExpressionInsectMutationPromoterpolyhedrinAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVAX1-cGAS
Plasmid#196624PurposeExpress mouse cGAS protein in mammalian cells.DepositorInsertcGAS (Cgas Mouse)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-APP751
Plasmid#193772PurposeEntry vector for human APP isoform having 751 amino acid residuesDepositorInsertAPP (APP Human)
UseTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-PARP1-Apex2–eGFP
Plasmid#211555PurposeA piggyBac vector containing CMV-PARP1-Apex2-eGFP-IRES-Neo cassette.DepositorInsertPARP1 (PARP1 Human)
UsePiggybacTagsApex2-eGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-3xMBT
Plasmid#46987PurposeInducible expression of 3xMBT from L3MBTL1 as a GST fusion protein in E.coliDepositorInsertTriple MBT domain from human L3MBTL1 (L3MBTL1 Human)
UseTagsGST and PreScission cleavage siteExpressionBacterialMutationcontains amino acids 190–530 of accession NP_0562…PromotertacAvailable sinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-3xMBT_D355N
Plasmid#46988PurposeInducible expression of 3xMBT (D355N) from L3MBTL1 as a GST fusion protein in E.coliDepositorInsertTriple MBT domain from human L3MBTL1 (L3MBTL1 Human)
UseTagsGST and PreScission cleavage siteExpressionBacterialMutationMutation aspartic acid 355 to asparagine (D355N);…PromotertacAvailable sinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-APPSw;T719N
Plasmid#177094PurposeFor lentiviral transduction of APPsw; T719NDepositorInsertAPPsw;T719N (APP Human)
UseLentiviralTagsExpressionMutationT719N, K651N and M652L (please see depositor comm…PromoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-DEST-RNF2
Plasmid#31216DepositorInsertRNF2 (RNF2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceApril 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Δ18
Plasmid#164436PurposeEncodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus productionDepositorInsertSARS-CoV-2 Spike (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTBL210
Plasmid#126451PurposeExpresses Cas9-XTEN-TdT in mammalian cells.DepositorInsertCas9-XTEN-TdT (DNTT Streptococcus pyogenes, Human)
UseTagsExpressionMammalianMutationSee depositor comments belowPromoterCMVAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-TRPS1-P2A-mCherry
Plasmid#203851PurposeTet-inducible lentiviral plasmid expressing TRPS1-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertTRPS1 (TRPS1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28(+)-NudC
Plasmid#175022PurposePurification of NudC enzymeDepositorInsertnudC
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
YA1529: pAAV_hSyn-QuasAr3-P2A-CheRiff
Plasmid#107700Purposein vivo voltage imagingDepositorInsertQuasAr3-P2A-CheRiff
UseAAVTagsExpressionMammalianMutationPromoterhuman synapsin IAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-hDND1
Plasmid#70070PurposeGateway entry vector for human DND1 with stop codonDepositorInsertDnd1 (DND1 Human)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVCT-Dnd1-EGFP
Plasmid#106343PurposeLentiviral vector, mammalian expression of Dnd1-EGFP fusion proteinDepositorInsertDnd1-EGFP (Dnd1 Mouse, Synthetic)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBGE3.0
Plasmid#165988PurposeMulti-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Nm/Kan resistance.DepositorInsertsRK2/RP4 origin of transfer
repA1B1C1
nourseothricin N-acetyl transferase
neomycin resistance
UseSynthetic Biology; Bacterial and yeast cloning; c…TagsExpressionMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRepair-mScI-CTNNB1
Plasmid#153431PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mScarlet-iDepositorInsertCTNNB1 homology arms and mScI coding sequence (CTNNB1 Human)
UseCRISPR; Hdr donor templateTagsmScarlet-iExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP C1-Eps8 ∆cap
Plasmid#74786Purposemammalian expression of the capping activity mutant Eps8 fused to GFPDepositorInsertEps8 (Eps8 Mouse)
UseTagsExpressionMammalianMutationV689D, L693DPromoterAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only