Addgene

We narrowed to 6,564 results for: ski

Showing: 3461 - 3480 of 6564 results

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pGEX PLCg1(NC)-SH2

Plasmid

#46471

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

PLCg1(NC) (PLCG1 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 542-759

Promoter

Tac

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Ash2L full

Plasmid

#15548

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

Ash2L (ASH2L Human)

Use

Tags

Expression

Mammalian

Mutation

R622H (Please see depositor comments below)

Promoter

Available since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only

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pCDH-SIRT2-Flag

Plasmid

#102624

Purpose

Expresses full length SIRT2 isoform 1 in mammalian cells

Depositor

Hening Lin

Article

Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

Insert

SIRT2 (SIRT2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

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myc-KIF5C

Plasmid

#127618

Purpose

N-terminal myc-tagged mouse KIF5C

Depositor

Josef Kittler

Article

Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

Insert

KIF5C (Kif5c Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe N-Ras 61K

Plasmid

#12543

Depositor

Channing Der

Article

Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

Insert

NRas 61K (NRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

61K constitutively activated.

Promoter

Available since

Sept. 8, 2006

Availability

Academic Institutions and Nonprofits only

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pLVX Flag RagC W115R

Plasmid

#112759

Purpose

express Flag-RagC (W115R mutant)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagC (W115R) (RRAGC Human)

Use

Lentiviral

Tags

Flag

Expression

Mutation

W115R

Promoter

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_05

Plasmid

#194666

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_05 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_05

Use

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TRC.mKO2

Plasmid

#85208

Purpose

Backbone for lentiviral gene silencing with monomeric Kusabira-Orange2 expression

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pSLQ10715

Plasmid

#183960

Purpose

U6-crOct4-CAG-hyperdCas12a-miniVPR

Depositor

Stanley Qi

Article

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Inserts

HyperdCas12a
crRNA targeting Oct4

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D832A, D156R, E292R, D235R, D350R

Promoter

CAG and U6

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSLIK-Rbfox2-rABE-Flag

Plasmid

#191384

Purpose

Doxycycline inducible expression of Rbfox2-rABE

Depositor

Stephen Floor

Article

Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

Insert

Rbfox2-rABE (RBFOX2 Synthetic, Human)

Use

Lentiviral

Tags

Flag

Expression

Mutation

Promoter

tet-inducible promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA SF-HERC2 F4

Plasmid

#55787

Purpose

Expresses N-terminal 3xFLAG/STREP tagged HERC2(2600-3600aa)

Depositor

David Chan

Article

Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

Insert

3xFLAG tagged HERC2 (2600-3600aa) (HERC2 Human)

Use

Tags

3xFLAG and STREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-FRT/T0-eGFPnls-53BP1 1220-1631 WT

Plasmid

#60814

Purpose

mammalian expression vector

Depositor

Daniel Durocher

Article

Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

Insert

53BP1 (TP53BP1 Human)

Use

Tags

eGFP-NLS (SV40)

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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SiC-V2-Cas9G7

Plasmid

#133043

Purpose

Doxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.

Depositor

Sriram Neelamegham

Article

Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

Insert

Tet repressor

Use

Lentiviral

Tags

Cerulean

Expression

Mutation

Promoter

Available since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRA3 CTF-FLAG

Plasmid

#217680

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRA3 (ADGRA3 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-737

Promoter

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRA2 CTF-FLAG

Plasmid

#217679

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRA2 (ADGRA2 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-746

Promoter

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_09

Plasmid

#194670

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_09 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_09

Use

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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CRY2(L348F)-CreN

Plasmid

#75368

Purpose

Expresses mCherry tag, then IRES, then Arabidopsis CRY2 (with L348F mutation) fused to N-terminal fragment of Cre DNA recombinase

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CRY2 (CRY2 Mustard Weed)

Use

Tags

Cre recombinase, residues 19-104

Expression

Mammalian

Mutation

L348F

Promoter

CMV

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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myc-KIF5B

Plasmid

#127617

Purpose

N-terminal myc-tagged mouse KIF5B

Depositor

Josef Kittler

Article

Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

Insert

KIF5B (Kif5b Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_01-mEGFP

Plasmid

#194681

Purpose

CMV driven expression of the calcium recorder Caprola_01 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_01-mEGFP

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pHS1861

Plasmid

#205974

Purpose

FLAG-SV40 NLS-Cas8-HNH from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas8-HNH

Use

Tags

FLAG-SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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