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Addgene
Showing: 3461 - 3480 of 16066 results

  1. pcDNA3-mCherry-TSC2(AA)

    Plasmid
    #192442
    Purpose
    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation.
    Depositor
    Jin Zhang
    Article
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Insert
    mCherry-TSC2(S939A,T1439A) (TSC2 Human)
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    192442-plasmid-map-sequence-id-379128

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  4. AAVS1_puro_CAG_HexaPro

    Plasmid
    #164077
    Purpose
    Targeted integration of SARS-CoV-2 spike HexaPro variant into the AAVS1 genomic safe harbor locus
    Depositor
    Yannick Doyon
    Article
    Rivest et al protocols.io 2021
    Insert
    SARS-CoV-2 S HexaPro (S Severe acute respiratory syndrome coronavirus 2)
    Use
    CRISPR
    Tags
    2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CAG
    Availability
    Industry, Academic Institutions, and Nonprofits
    164077-plasmid-map-sequence-id-320680

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  5. pcDNA3-DrFLINC-AKAR-Kras

    Plasmid
    #182334
    Purpose
    Genetically encoded fluorescent biosensor for super-resolution imaging of PKA activity; targeted to the plasma membrane. Compatible with multiplexed super-resolution imaging using a Dronpa-tagged POI.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    DrFLINC-AKAR
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    182334-plasmid-map-sequence-id-363289

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  6. UQ6139

    Bacterial Strain
    #37177
    Depositor
    Gary Roberts
    Bacterial Resistance
    Ampicillin and kanamycin
    Species
    Burkholderia xenovorans
    Availability
    Academic Institutions and Nonprofits only

  19. MAP3K6-TAP

    Plasmid
    #69727
    Purpose
    Express tandem tagged MAP3K6 in cells
    Depositor
    Daniel Liebler
    Insert
    Apoptosis signal-regulating kinase 2 (MAP3K6 Human)
    Use
    Tags
    HA-V5 tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    69727-plasmid-map-sequence-id-128182

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  20. Flag-neo-PP1-Gamma

    Plasmid
    #171270
    Purpose
    Mammalian expression of 3x-Flag-tagged human PP1 gamma catalytic subunit isoform 1
    Depositor
    Steven Balk
    Insert
    3x-Flag-tagged human PP1 gamma catalytic subunit isoform 1 (PPP1CC Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    171270-plasmid-map-sequence-id-335789

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Showing: 3461 - 3480 of 16066 results