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Plasmid#219497PurposepCDF-Duet deriviative with sfGFP flanked by 2 BsaI sites downstream of the first T7 promoter and independent msr and RT downstream of the second T7 promoterDepositorInsertsfGFP, Eco1 msr, Eco1 RT
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGD248
Plasmid#219498PurposeTemperature sensitive replication (repA101ts) backbone encoding the retron recombineering operon (ncRNA, RT, CspRecT, mutLE32K)DepositorInsertEco1 ncRNA (rpoB donor), Eco1 RT, CspRecT, mutLE32K
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Parkin
Plasmid#213547PurposeTransient mammalian expresssion of untagged ParkinDepositorInsertParkin (PRKN Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
EGFP-GRAM1b
Plasmid#211494PurposeExpression of EGFP-tagged GRAM domain of GRAMD1b (EGFP-GRAM1b) in mammalian cellsDepositorInsertGRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-Celf4/Brunol4 [N446/80R]
Plasmid#206584PurposeMammalian Expression Plasmid of anti-Celf4/Brunol4 (Human). Derived from hybridoma N446/80.DepositorInsertanti-Celf4/Brunol4 (Homo sapiens) recombinant Mouse monoclonal antibody (CELF4 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVTHM-mSox17-3xflag
Plasmid#206377PurposeExpresses 3xflag fused mouse SOX17 in mammalian cells, for lentivirus generation.DepositorInsertSox17 (Sox17 Mouse)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3272
Plasmid#144748PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPRDM4 (PRDM4 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-mNG2(1-10)
Plasmid#190209PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNeonGreen2(1-10)
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
p5419
Plasmid#168186Purposebacteria reporterDepositorInsertPBAD::tag-rfp-T PtetA::sfgfp
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_FSCN1-Halo
Plasmid#175343PurposeProduction of HaloTag fusion proteinDepositorInsertFSCN1 (FSCN1 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDH013
Plasmid#157638PurposesMHT-113 fused to GFP and RFPDepositorInsertssMHT(1-113)-SYNZIP17-GFP
RFP-SYNZIP18-sMHT(114-23)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
SnFR-gamma2
Plasmid#165495PurposeGreen fluorescent glutamate reporter fused to TARP gamma-2 for mammalian expression.DepositorInsertiGluSnFR extracellular domain fused to Stargazin (gamma-2) via NETO2 TM domain (Cacng2 Rat, Synthetic)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTNT-pT7-tetR-mCherry
Plasmid#140870PurposeT7 promoter driven synthesis of TetR-mCherry fusion proteinDepositorInsertTetR-mCherry expressed with T7 promoter
UseSynthetic BiologyTagsmCherryExpressionBacterialMutationPromoterT7Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-clbH-A1
Plasmid#114149PurposeExpresses ClbH-A1DepositorInsertclbH-A1
UseTags6xHis-thrombin-T7 tagExpressionBacterialMutationContains only the A1 domain of ClbHPromoterAvailable sinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
HL 1128
Bacterial Strain#60372PurposeLim Lab StrainDepositorBacterial ResistanceNoneAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 hygro
Plasmid#24150Purpose3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNAAvailable sinceMarch 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T MDM2 WT
Plasmid#16237DepositorInsertMDM2 (MDM2 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMXs-3XHA-EGFP-OMP25
Plasmid#83356PurposeFor tagging mitochondria with HA epitopes (HA-MITO construct)DepositorInsert3XHA-EGFP-OMP25
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-EcSTH-FLAG
Plasmid#218628PurposeThis plasmid contains human codon optimized sequence of EcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.DepositorInsertEcSTH
UseLentiviral and Synthetic BiologyTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ASAP3-WPRE
Plasmid#132331Purposeexpresses ASAP3(ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vectorDepositorInsertASAP3
UseAAVTagsExpressionMammalianMutationPromoterhSynapsinAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only