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Showing: 3441 - 3460 of 14503 results

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pCMV‐ABEmaxNGX‐loop

Plasmid

#187061

Purpose

ABEmax-SpCas9-NGX-loop

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

ABEmaxNGX‐loop

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_ABE‐NGC

Plasmid

#187056

Purpose

ABEmax-SpCas9-NGC

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

ABE‐NGC

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET30-T7-lacO-His-sfGFP

Plasmid

#180754

Purpose

pET30 vector with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Article

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Use

Tags

strep tag

Expression

Bacterial

Mutation

G140C- Please see depositor comments

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP.N3-myc-SLC35A2

Plasmid

#186285

Purpose

transient expression of N-terminal myc tagged SLC35A2 isoform c/UGT1 in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Li et al Glycobiology. 2019 Jun 1;29(6):490-503.

Insert

SLC35A2 (SLC35A2 Human)

Use

Tags

MYC

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAW2-Smed-Slit-1

Plasmid

#182264

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Slit-1

Depositor

Daniel Lobo

Article

Wolff et al STAR Protoc. 2022 Jun 14;3(3):101458. do

Insert

Smed-Slit-1

Use

Gibson cloning vector for synthesis of single and…

Tags

Expression

Mutation

Promoter

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK23 pCAS-Tyr-[gRNA: 6=ARS416] (SplitHygR, AmpR)

Plasmid

#179007

Purpose

Sp.Cas9 and gRNA yeast expression vector with ARS416 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK22 pCAS-Tyr-[gRNA: 5=ARS308] (SplitHygR, AmpR)

Plasmid

#179006

Purpose

Sp.Cas9 and gRNA yeast expression vector with ARS308 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK19 pCAS-Tyr-[gRNA: 2=FF21] (SplitHygR, AmpR)

Plasmid

#179003

Purpose

Sp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK18 pCAS-Tyr-[gRNA: 1=FF18] (SplitHygR, AmpR)

Plasmid

#179002

Purpose

Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK17 pCAS-Tyr-[gRNA: 7=HIS3] (SplitKanR, AmpR)

Plasmid

#179001

Purpose

Sp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK16 pCAS-Tyr-[gRNA: 6=ARS416] (SplitKanR, AmpR)

Plasmid

#179000

Purpose

Sp.Cas9 and gRNA yeast expression vector with ARS416 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK15 pCAS-Tyr-[gRNA: 5=ARS308] (SplitKanR, AmpR)

Plasmid

#178999

Purpose

Sp.Cas9 and gRNA yeast expression vector with ARS308 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-sg-CMV152

Plasmid

#179916

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 152.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-sg-CMV51

Plasmid

#179915

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 51.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-sg-CMV162

Plasmid

#179917

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 162.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-sg-mGreenLantern332

Plasmid

#179914

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern fluorescent protein site 332.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

mGreenLantern sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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PX552-rScn2a-3xV5-Syn-miRFP670

Plasmid

#182566

Purpose

For rat Nav1.2 knockin with 3xV5 at the extracellular loop

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

rat Scn2a gRNA and 3xV5 donor DNA
miRFP670

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Syn and U6

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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piggybac-Syn-mScn8a_ICL1-sfGFP-P2A-mCherry

Plasmid

#182560

Purpose

Expresses Nav1.6 ICL1 tagged with sfGFP-P2A-mCherry

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn8a ICL1
sfGFP-P2A-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Syn

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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CAG.mCherry-INBox-eDHFR

Plasmid

#176202

Purpose

Single INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

mCherry-INBox-eDHFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CAG.MCAK-mCherry-eDHFR

Plasmid

#176204

Purpose

MCAK (179-583) recruitment responsible for targeted microtubule depolymerization using TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

MCAK-mCherry-eDHFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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