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Plasmid#161602PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XIII-1, (Chr XIII: 306804..306823)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only
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pCfB9363(XVI-1_Markerfree_BackBone)
Plasmid#161604PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XVI-1, (Chr XVI: 406267..406286)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG.INBox-mCherry-INBox-eDHFR
Plasmid#176203PurposeDouble INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization systemDepositorInsertINBox-mCherry-INBox-eDHFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG.mCherry-INBox-eDHFR
Plasmid#176202PurposeSingle INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization systemDepositorInsertmCherry-INBox-eDHFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL96
Plasmid#168330PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertSi514802027
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG.mCherry-INBox(AAA)-eDHFR
Plasmid#176207PurposeINBox TSS(892-894)-mutant replaced by AAA responsible for targeted Aurora B recruitment without activation using TMP-Halo dimerization systemDepositorInsertmCherry-INBoxAAA-eDHFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
tcTRP24sub8_Cap
Plasmid#175797Purposede novo thick circular tandem repeat protein capped to prevent multimerizationDepositorInserttcTRP24sub8_Cap
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
SH2-free
Plasmid#175800PurposeHuman Nck2 SH2 domainDepositorInserthuman Nck2 SH2
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
tcTRP9
Plasmid#175793Purposede novo thick circular tandem repeat proteins with 9 repeatsDepositorInserttcTRP9
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Mdn1-FL
Plasmid#171602PurposeExpresses full length S. pombe Mdn1 in insect cells.DepositorInsertMdn1
UseTagsHis6 tagExpressionInsectMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-2
Plasmid#160702Purposegpdh promoter from Trichoderma virens bordered by BsaI restriction sitesDepositorInsertgpdh
UseSynthetic BiologyTagsExpressionMutationIncorporation of BsaI restriction sitesPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
Rap-MESA-20-Tev
Plasmid#84555PurposeMESA protease chain with rapamycin FKBP ectodomain, 20 extracellular linkers, and Tev proteaseDepositorInsertRap-MESA-FKBP-20-Tev
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
MTK234_038
Plasmid#123917PurposeEncodes the spCas9 pSV40 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA-pSV40
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK234_043
Plasmid#123913PurposeEncodes the spCas9 pSV40 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA-pSV40
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK8_005
Plasmid#123885PurposeEncodes the F1 Kanamycin resistance cassette as a type 8 part to be used in the MTK systemDepositorInsertF1-KanR-ColE1
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK234_047
Plasmid#124055PurposeEncodes the spCas9 mScarlet (site 2) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertmScarlet sgRNA 2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_048
Plasmid#124056PurposeEncodes the spCas9 mScarlet (site 3) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertmScarlet sgRNA 3
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_049
Plasmid#124057PurposeEncodes the spCas9 mScarlet (site 4) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertmScarlet sgRNA 4
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3b_023
Plasmid#123953PurposeEncodes human RelA as a Type 3b part to be used in the MTK systemDepositorInsertRelA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3b_025
Plasmid#123954PurposeEncodes synTF fused to VP16 activation domain as a Type 3b part to be used in the MTK systemDepositorInsertSynTF_VP16
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only