Addgene

We narrowed to 6,285 results for: sms

Showing: 3401 - 3420 of 6285 results

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pLV_TRET_Ngn2‐2A‐Ngn1

Plasmid

#61471

Purpose

3rd generation lentiviral vector; TetON promoter driving mouse Ngn2 and Ngn1

Depositor

Ron Weiss

Article

Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

Inserts

Ngn2 (Neurog2 Mouse)
Neurog1 (Neurog1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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PT3.5/CMV-EGFRvIII

Plasmid

#20280

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

EGFRvII (EGFR Human)

Use

Transposon

Tags

Expression

Mutation

See depositor comments below.

Promoter

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pMZ7

Plasmid

#223180

Purpose

pORI28 derivative with lacZ and upp gene inserted into the backbone; for generating gene-specific knockouts.

Depositor

Paul Blainey

Article

Zhu et al bioRxiv [Preprint]. 2023 Dec 30:2023.12.

Type

Empty backbone

Use

Chromosomal integration

Tags

Expression

Mutation

Promoter

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pTMB341_ZFcharm_Prnp_SCR

Plasmid

#220844

Purpose

AAV genome expressing ZFcharm targeting Prnp; scrambled self-silencing binding site

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-Lepr_Short-IRES-eGFP

Plasmid

#196935

Purpose

Doxycycline inducible expression of short Lepr (no signaling) with eGFP marker

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

Lepr (Lepr Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

short isoform, lack of signaling casacade

Promoter

TRE

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-mPD-L1-T2A-GFP

Plasmid

#205455

Purpose

lentiviral plasmid for expression of mouse PD-L1

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mPD-L1 (Cd274 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

UBC

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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AbVec2.0-mIghg2b

Plasmid

#127155

Purpose

Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2b isotype

Depositor

Hedda Wardemann

Article

Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

Insert

Ighg2b (Ighg2b Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV IE1 gene promoter

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6p1-GST-Rnf31

Plasmid

#63134

Purpose

Expression of human RNF31/HOIP codon optimized for E. coli and insect cells

Depositor

Titia Sixma

Article

Smit et al EMBO J. 2012 Oct 3;31(19):3833-44. doi:

Insert

Rnf31 (RNF31 Human, Synthetic)

Use

Tags

GST

Expression

Bacterial

Mutation

Synthetic based on human protein sequence

Promoter

tac

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only
MSCV-IRES-GFP ASXL1 (p.G646 Wfs *12) 3x FLAG

Plasmid

#81021

Purpose

Cancer-associated ASXL1 truncation mutant in retroviral vector

Depositor

Anjana Rao

Article

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

ASXL1 (ASXL1 Human)

Use

Tags

3X FLAG

Expression

Mammalian

Mutation

p.G646 Wfs *12 truncation

Promoter

5’LTR

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only
PB713B-pCMV-MITD-NeoMini-T2A-tCD19-WPRE

Plasmid

#174610

Purpose

PiggyBack transposon expressing a fusion of a tandem minigene antigens in KRAS, BRAF and CMV fused to an endosomal targeting sequence and translationally linked to human truncated CD19

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

human CD19, antigen minigene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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AttB_ACE2_IRES-mCherry-H2A-P2A-PuroR

Plasmid

#171594

Purpose

Strong human ACE2 abundance from Bxb1 landing pad

Depositor

Kenneth Matreyek

Article

Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

Inserts

ACE2 (ACE2 Human)
mCherry
H2A
Pac

Use

Synthetic Biology; Promoterless bxb1 dna recombin…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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AAV SYN flex PSAM4 GlyR IRES EGFP

Plasmid

#119741

Purpose

Chemogenetics Cre-dependent expression plasmid

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Has Service

AAV5 and AAV9

Insert

PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)

Use

AAV

Tags

Expression

Mammalian

Mutation

L131G, Q139L, Y217F

Promoter

synapsin

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLM-mCherry-Klf4

Plasmid

#23243

Depositor

Michel Sadelain

Article

Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

Insert

mCherry_2A_KLF4 (KLF4 synthetic fluorescent protein, Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pCAGGS 3xFLAG Mbnl2

Plasmid

#217032

Purpose

Overexpression of Mbnl2 in mammalian cells

Depositor

Chaolin Zhang

Article

Weyn-Vanhentenryck et al Nat Commun. 2018 Jun 6;9(1):2189. doi: 1

Insert

Mbnl2 (Mbnl2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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FAK2_HUMAN_D0

Plasmid

#79747

Purpose

This plasmid encodes the kinase domain of FAK2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

Markus Seeliger, John Chodera, Nicholas Levinson

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

FAK2 (PTK2B Human)

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
shFGF2#2

Plasmid

#157668

Purpose

Tet inducible knockdown of FGF2

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Fibroblast growth factor 2 (FGF2 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-hSNRPN-R-loop

Plasmid

#134902

Purpose

Generation and purification of R-loops for in vitro biochemistry

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Insert

hSNRPN promoter (SNRPN Human)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES-GFP ASXL1 (1-479) 3x FLAG

Plasmid

#81023

Purpose

Cancer-associated ASXL1 truncation mutant in retroviral vector

Depositor

Anjana Rao

Article

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

ASXL1 (ASXL1 Human)

Use

Tags

3X FLAG

Expression

Mammalian

Mutation

Contains ASXL1 amino acids 1-479

Promoter

5’LTR

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only
pGEX6P1-3xMBT

Plasmid

#46987

Purpose

Inducible expression of 3xMBT from L3MBTL1 as a GST fusion protein in E.coli

Depositor

Or Gozani

Article

Moore et al Mol Cell. 2013 May 9;50(3):444-56. doi:

Insert

Triple MBT domain from human L3MBTL1 (L3MBTL1 Human)

Use

Tags

GST and PreScission cleavage site

Expression

Bacterial

Mutation

contains amino acids 190–530 of accession NP_0562…

Promoter

tac

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES-GFP ASXL1 (p.Y591X) 3x FLAG

Plasmid

#81022

Purpose

Cancer-associated ASXL1 truncation mutant in retroviral vector

Depositor

Anjana Rao

Article

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

ASXL1 (ASXL1 Human)

Use

Tags

3X FLAG

Expression

Mammalian

Mutation

p.Y591X truncation

Promoter

5’LTR

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only