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Plasmid#59032Purposeexpresses CKK domain of CAMSAP2 in E. coliDepositorInsertCAMSAP2 CKK domain (CAMSAP2 Human)
UseTagsHis, Strep, TEV, and sfGFPExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-mEGFP-HMGB1-K184Rfs
Plasmid#194544PurposeBacterial Expression of 6xHis mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion ProteinDepositorInsertHMGB1 (HMGB1 Human)
UseTagsmEGFPExpressionBacterialMutationK184Rfs*44PromoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
Arabidopsis thaliana Atm3 d80 pET21a+
Plasmid#173045PurposeExpresses AtAtm3 in E.coliDepositorInsertArabidopsis thaliana Atm3 (ABCB25 Mustard Weed)
UseTags6xHis tagExpressionBacterialMutationwith N-terminal 80 amino acids deletionPromoterAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)-Hcp1
Plasmid#87737PurposeBacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
UseTags6xHis-V5-TEV and Hcp1ExpressionBacterialMutationdeleted amino acids 1-100PromoterT7Available sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-28a+GFP-CAMSAP1 CKK
Plasmid#59031Purposeexpresses CKK domain of CAMSAP1 in E. coliDepositorInsertCAMSAP1 CKK domain (CAMSAP1 Human)
UseTagsHis, Strep, TEV, and sfGFPExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET29-hMIGA2(170-593)-6xhis
Plasmid#192834PurposeExpress codon-optimized human MIGA2 fragment 170-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteriaDepositorInsertCodon-optimized human Mitoguardin 2 (MIGA2 Human)
UseTags6xhisExpressionBacterialMutationPromoterT7Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET23A-Fucosidase-E1_10125-Ruminococcus_gnavus-His
Plasmid#207665PurposeExpresses fucosidase E1_10125 from Ruminococcus gnavus in BL21 cells, purified using His-tagDepositorInsertFucosidase E1_10125
UseTagsHis-tagExpressionBacterialMutationamino acids 34-584 were usedPromoterT7 promoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) NRP1 b1b2 (273-586)
Plasmid#162733PurposeBacterial expression the b1b2 tandem domains from the rat Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.DepositorInsertHuman NRP1 b1b2 domains (residues 273-586)
UseTags6HisExpressionBacterialMutationCodon optimisedPromoterAvailable sinceMarch 29, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-28a(+)-His(6)-TEV-AtPCO4-1(wt)
Plasmid#191801PurposeFull length wt AtPCO4 with Nt-His tagDepositorInsertAtPCO4-1
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET empty polycistronic destination vector (2Z)
Plasmid#29776DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GgVcl 1-1066 (full length)
Plasmid#46171DepositorInsertVcl (VCL Chicken)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SARS-CoV2-ORF3b (S23Q)
Plasmid#165088Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 ORF3b (S23Q)
UseTagsExpressionBacterialMutationStop codon to glutamine at 23rd amino acidPromoterAvailable sinceFeb. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pF710-pETDuet-1-Hs-delta19-ETFalpha-noHis wt
Plasmid#85111Purposeexpression of recombinant human mature (delta19) ETFalpha wt without tagDepositorInserthuman mature (delta19) ETFalpha (ETFA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
HOXC9 (murine) HIS-tag pET
Plasmid#8559DepositorInsertHOXC9 (Hoxc9 Mouse)
UseTagsHis and T7ExpressionBacterialMutationmakes protein by WesternPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
His-SUMO- flag-hDDI2-D252N_pET11a
Plasmid#169123Purposeexpression of proteinDepositorInserthDDI (DDI2 Human)
UseTagsExpressionBacterialMutationD252NPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pet151D-TOPO ALIX Bro1-V (1-702)
Plasmid#80642PurposeResidues 1-702 of ALIX; Bacterial Expression Vector; TEV cleavable N-term His-tag; Sundquist Lab Internal ID: WISP10-648DepositorInsertALIX Bro1-V (PDCD6IP Human)
UseTagsHis TagExpressionBacterialMutationResidues 1-702PromoterAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-sfGFP-DocI
Plasmid#58708PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsertsfGFP-DocI
UseTagsHIS, HRV 3C, and ybbRExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-HIS-ddFLN4-K10
Plasmid#101718PurposeK10 peptide with ddFLN4 fingerprint domain and N-terminal ybbr tag for covalent immobilization - resulting in high forcesDepositorInsertddFLN4 fingerprint, K10 peptide (KRT10 Human, Dictyostelium discoideum)
UseTagsHIS and ybbrExpressionBacterialMutationC18S in ddFLN4PromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-FLAG-SpCas9-HF1-plus
Plasmid#126775PurposeExpression of increased fidelity SpCas9-HF1-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1.DepositorInsertSpCas9-HF1-plus
UseCRISPRTags3xFLAG, 6xHis, MBP, and NLSExpressionBacterialMutationN497A; Q695A; Q926A; amino acids 1005-1013 replac…PromoterT7Available sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21c-His-Xa-Gluc-Xa-C9D-TGinsert
Plasmid#124660Purposereporter protein which emits bright blue fluorescenceDepositorInsertGaussia luciferase-C9D-TGinsert
UseTagsIEGRTMGDDDGDDDGDDD(FactorXa,TMG,C9D-Tag) and MHHH…ExpressionBacterialMutationchanged glutamic acid 100 to alanine, and glycine…PromoterT7 promoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only