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Plasmid#111771PurposeBicistronic construct: Co-express two genes by 2A peptidesDepositorInsertMef2c-tPT2A-GFP (Mef2c Mouse)
UseCloning intermediateTagsGFPExpressionMutationPromoterT7Available sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-Trastuzumab-IgA1/κ
Plasmid#61888PurposeExpresses HER2/neu receptor specific humanized IgA1/κ antibody isotype (Trastuzumab-IgA1/κ)DepositorInsertsHER2/neu receptor specific humanized Alpha 1 heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette
UseTagsExpressionMammalianMutationPromotermEF1 Prom and rEF1 PromAvailable sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-Trastuzumab-IgG2/κ
Plasmid#61884PurposeExpresses HER2/neu receptor specific humanized IgG2/κ antibody isotype (Trastuzumab-IgG2/κ)DepositorInsertsHER2/neu receptor specific humanized Gamma 2 heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette
UseTagsExpressionMammalianMutationPromotermEF1 Prom and rEF1 PromAvailable sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-Trastuzumab-IgA2/κ
Plasmid#61889PurposeExpresses HER2/neu receptor specific humanized IgA2/κ antibody isotype (Trastuzumab-IgA2/κ)DepositorInsertsHER2/neu receptor specific humanized Alpha 2 heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette
UseTagsExpressionMammalianMutationPromotermEF1 Prom and rEF1 PromAvailable sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-Trastuzumab-IgG4/κ
Plasmid#61887PurposeExpresses HER2/neu receptor specific humanized IgG4/κ antibody isotype (Trastuzumab-IgG4/κ)DepositorInsertsHER2/neu receptor specific humanized Gamma 4 heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette
UseTagsExpressionMammalianMutationPromotermEF1 Prom and rEF1 PromAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTTCC
Plasmid#164264PurposeExpresses a thermostable Cas9 enzyme and gRNA in Thermus thermophilusDepositorInsertsCas9
gRNA scaffold
UseTagsExpressionBacterialMutationPromoterPcel and trcAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
p221a-GUS
Plasmid#71263PurposeEntry clone containing the GUS enzyme. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertBeta-glucuronidase
UseGatewayTagsExpressionMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-Trastuzumab-IgM/κ
Plasmid#61881PurposeExpresses HER2/neu receptor specific humanized IgM/κ antibody isotype (Trastuzumab-IgM/κ)DepositorInsertsHER2/neu receptor specific humanized Mu heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette
UseTagsExpressionMammalianMutationPromotermEF1 Prom and rEF1 PromAvailable sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
p2R3a-nosT
Plasmid#71272PurposeEntry clone containing nosT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertnopaline synthase terminator
UseGatewayTagsExpressionMutationPromoterAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-Trastuzumab-IgG3/κ
Plasmid#61885PurposeExpresses HER2/neu receptor specific humanized IgG3/κ antibody isotype (Trastuzumab-IgG3/κ)DepositorInsertsHER2/neu receptor specific humanized Gamma 3 heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette
UseTagsExpressionMammalianMutationPromotermEF1 Prom and rEF1 PromAvailable sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 HCoV-OC43 NSP1_nostop
Plasmid#168687PurposeGateway-compatible Entry vectorDepositorInsertNSP1 (ORF1ab )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
p2R3a-Tq2CFP-OcsT
Plasmid#71268PurposeEntry clone containing Turqoise2. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertTq2CFP
UseGatewayTags4xGly linker and octaline synthase terminatorExpressionMutationPromoterAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
p2R3a-3AT
Plasmid#71273PurposeEntry clone containing 3AT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertT3A ribulose-1,5-bisphosphate carboxylase 3A subunit terminator
UseGatewayTagsExpressionMutationPromoterAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-IL6.Pro
Plasmid#79491PurposeMultiSite Gateway entry clones, first fragment, (attL1, attR5) Human IL6 promoterDepositorInsertIl6 Promoter (IL6 Synthetic, Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterIL6 PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-Cas9-V5
Plasmid#87905PurposeCloning vectorDepositorInsertCas9-V5
UseCloning vectorTagsV5ExpressionMutationHuman codon optimizedPromoterV5Available sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-dV-IgE/κ
Plasmid#61878PurposeHuman immunoglobulin epsilon antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) coloniesDepositorInsertsHuman immunoglobulin Epsilon constant region
Human immunoglobulin kappa constant region
UseTagsExpressionMammalianMutationDeleted Variable regionPromotermEF1 Prom and rEF1 PromAvailable sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 HCoV-OC43 NSP5_nostop
Plasmid#168690PurposeGateway-compatible Entry vectorDepositorInsertNSP5 (ORF1ab )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 HCoV-OC43 NSP12_nostop
Plasmid#168696PurposeGateway-compatible Entry vectorDepositorInsertNSP12 (ORF1ab )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 HCoV-HKU1 NSP1_nostop
Plasmid#168570PurposeGateway-compatible Entry vectorDepositorInsertNSP1 (ORF1ab )
UseOtherTagsExpressionMutationPromoterAvailable sinceJan. 20, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
B270 (plasmid expressing ATP1A1 sgSTOP and containing an empty sgRNA-expression cassette)
Plasmid#100716PurposeB52 plasmid expressing ATP1A1 sgSTOP (cloned in BsmBI site) and containing an empty sgRNA-expression cassette (use BbsI for cloning)DepositorInsertsgSTOP targeting ATP1A1 (cloned using BsmBI)
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promotersAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only