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PERK 2: PERK.K618A.9E10.pCDNA
Plasmid#21815DepositorInsertPERK (K618A) (Eif2ak3 Mouse)
UseTagsMyc (9E10)ExpressionMammalianMutationkinase dead K618A mutantPromoterAvailable sinceOct. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAD/CMV/V5-DEST-Cdc42-2G
Plasmid#68812PurposeFRET-based biosensor reporting on endogenous Cdc42 activation (for Adenovirus production)DepositorInsertCdc42 activation reporter
UseAdenoviralTagsHis and MycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_sg61-PDGFR_EF1a_eGFP
Plasmid#186310PurposePlasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line constructionDepositorInsertssg61-PDGFR
eGFP
UseTagsHis, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag3B-CCre
Plasmid#106369PurposeC-terminal part of the split-Cre SystemDepositorInsertCCre
UseCre/LoxTagsc-mycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PACT
Plasmid#50352Purposebacterial expression of PACTDepositorInsertPACT (PRKRA Human)
UseTagsHis and MycExpressionBacterialMutationPromoterT7Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_Z17-PDGFR_EF1a_mCherry
Plasmid#186303PurposePlasmid for inducible mammalian cell expression of the Z17 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction.DepositorInsertsZ17-PDGFR
mCherry
UseTagsHA, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PERK 3: PERK.dC.9E10.pCDNA
Plasmid#21816DepositorInsertPERK (C-term deleted) (Eif2ak3 Mouse)
UseTagsMyc (9E10)ExpressionMammalianMutationamino-acids 582-1081 deletedPromoterAvailable sinceJuly 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEFmEGFP-mDia2
Plasmid#25407DepositorInsertmDia2 (Diaph3 Mouse)
UseTagsEGFP, His, and MycExpressionMammalianMutationEncodes mDia2PromoterAvailable sinceAug. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-human-Caspase-1
Plasmid#183359PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-1 (CASP1 Human)
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJ3M-Mst1 K59R
Plasmid#12204DepositorUseTagsMycExpressionMammalianMutationCatalytically inactive: K59RPromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
Dynamitin-GFP
Plasmid#51419Purposeexpression of GFP-tagged wild-type dynamitin p50 in mammalian cellsDepositorInsertDynamitin (DCTN2 Chicken)
UseTagsGFP and mycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lmajor_gp63_10_0460_WT
Plasmid#171640PurposeExpression of wildtype L. major glcyoprotein-63 from chromosome 10 in mammalian cellsDepositorInsertLmjF.10.0460
UseTagsMyc-HisExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_sg30-PDGFR_EF1a_mCherry
Plasmid#186309PurposePlasmid for inducible mammalian cell expression of the sg30 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line constructionDepositorInsertssg30-PDGFR
mCherry
UseTagsHis, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lmajor_gp63_10_0460_E265A
Plasmid#171641PurposeExpression of catalytically inactive L. major glcyoprotein-63 (E265A) from chromosome 10 in mammalian cellsDepositorInsertLmjF.10.0460 E265A
UseTagsMyc-HisExpressionMammalianMutationE265APromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTriExEKAR2G2
Plasmid#40175DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS2-MT-mitfa
Plasmid#81231Purposeexpression of zebrafish mitfaDepositorInsertmitfa (mitfa Zebrafish)
UseZebrafish expressionTags6 myc tagsExpressionMammalianMutationcontains amino acids 5-412PromotersCMV IE94 promoterAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design1_mTFP_175_Venus_173
Plasmid#39830DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
HA.PKD.K/W
Plasmid#10809DepositorInsertPKD (PRKD1 Human)
UseTagsHA and Myc (inserted between amino acids 33&3…ExpressionMammalianMutationKinase dead mutant: K612W (Lys 612 to Trp) (bp183…PromoterCMVAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDBEcR/Bla
Plasmid#201556PurposePlasmid with SB ITRs for expressing DBEcR (3xmyc-tagged) under EF1α promoter; blasticidin resistance.DepositorInsertDBEcR
UseUnspecified; Sleeping beauty transposonTagsMycExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 13, 2023AvailabilityAcademic Institutions and Nonprofits only
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