Addgene

We narrowed to 489 results for: sp2

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pCMV-Tag 3A/WT myc-M1

Plasmid

#87719

Purpose

Expresses WT myc-M1 spastin isoform in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

M1 isoform

Promoter

CMV

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/WT M1

Plasmid

#89321

Purpose

expression of M1 C448Y spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted

Promoter

CMV

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/M1C448Y

Plasmid

#89319

Purpose

expression of M1 C448Y spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted, C448Y

Promoter

CMV

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pfTRAP

Plasmid

#50048

Purpose

Expresses plasmodium falciparum TRAP adhesion domains (26-299) in mammalian cells

Depositor

Timothy Springer

Article

Song et al Proc Natl Acad Sci U S A. 2012 Dec 26;10

Insert

plamodium falciparum TRAP (TRAP Synthetic)

Use

Tags

6Xhis tag

Expression

Mammalian

Mutation

C55G, N132S

Promoter

CMV

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only
pfTRAP(VWA)

Plasmid

#50049

Purpose

Expresses plasmodium falciparum TRAP VWA domain (41-240) in mammalian cells

Depositor

Timothy Springer

Article

Song et al Proc Natl Acad Sci U S A. 2012 Dec 26;10

Insert

pfTRAP(vwa) (TRAP Synthetic)

Use

Tags

6Xhis tag

Expression

Mammalian

Mutation

C55G, N132S

Promoter

CMV

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-Tag/M87 C448Y

Plasmid

#89320

Purpose

expression of M87 C448Y spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-460 bp deleted

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/WT SPAST with 5'UTR

Plasmid

#87731

Purpose

Expresses WT spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Tag/WT SPAST without 5'UTR

Plasmid

#87732

Purpose

Expresses WT spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

5'UTR deleted (1-190 bp)

Promoter

CMV

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMAL-EFSep

Plasmid

#34627

Depositor

Dieter Söll, Jesse Rinehart

Article

Park et al Science. 2011 Aug 26;333(6046):1151-4.

Insert

EF-Sep (tufB E. coli)

Use

Tags

6xHIS and MBP

Expression

Bacterial

Mutation

His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…

Promoter

Available since

March 1, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-Tag 3A/myc-M1L195V

Plasmid

#87716

Purpose

Expresses myc-M1L195V spastin isoform in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

M1 isoform; L195V

Promoter

CMV

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M1WT

Plasmid

#89327

Purpose

Inducible expression of WT M1 spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted

Promoter

Tet-responsive P tight

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M87 WT

Plasmid

#89328

Purpose

Inducible expression of WT M87 spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-460 bp deleted

Promoter

Tet-responsive P tight

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag 3A/myc-M1S46X

Plasmid

#87718

Purpose

Expresses myc-M1S46X spastin isoform in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

M1 isoform; S46X

Promoter

CMV

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag 3A/myc-M1S44L

Plasmid

#87717

Purpose

Expresses myc-M1S44L spastin isoform in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

M1 isoform; S44L

Promoter

CMV

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCA528 AMSHLP 16-218

Plasmid

#180609

Purpose

Bacterial expression for AMSHLP MIT domain residues 16-218. Internal ID: WISP20-83

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

AMSHLP residues 16-218

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 16-218

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn2b-R60f-BFP

Plasmid

#186608

Purpose

Encodes human Reticulon 2 disease mutant R60f fused to BFP

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 mutant R60f (RTN2 Human)

Use

Tags

BFP

Expression

Mammalian

Mutation

c.178insC (R60f)

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-Rtn2b-mNeon-TurboID

Plasmid

#186612

Purpose

Third generation lentiviral vector expressing full-length Reticulon 2 fused to mNeonGreen and V5-TurboID

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B (RTN2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn2b-NTD1-271-mNeon

Plasmid

#186601

Purpose

Encodes human Reticulon 2 isoform B N-terminal domain aa 1 to 271 fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B amino acids 1 to 271 (RTN2 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn2b-NTD1-135-mNeon

Plasmid

#186602

Purpose

Encodes human Reticulon 2 isoform B N-terminal domain aa 1 to 135 fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B amino acids 1 to 135 (RTN2 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Rtn2b-NTD1-60-mNeon

Plasmid

#186603

Purpose

Encodes human Reticulon 2 isoform B N-terminal domain aa 1 to 60 fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B amino acids 1 to 60 (RTN2 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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