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Plasmid#131112PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C399A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 399 muta…PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C393A-2xStrep
Plasmid#131113PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C393A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 393 muta…PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C355A-2xStrep
Plasmid#131114PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C355A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 355 muta…PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C344A-2xStrep
Plasmid#131115PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C344A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 344 muta…PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C341A-2xStrep
Plasmid#131116PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C341A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 341 muta…PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C301A-2xStrep
Plasmid#131117PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C301A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 301 muta…PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C298A-2xStrep
Plasmid#131118PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C298A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 298 muta…PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C295A-2xStrep
Plasmid#131119PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C295A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 295 muta…PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C289A-2xStrep
Plasmid#131120PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C289A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 289 muta…PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C244A-2xStrep
Plasmid#131121PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C244A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 244 muta…PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 200-429-2xStrep
Plasmid#130955PurposeExpresses KSHV ORF66 a.a. 200-429 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 200-429PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C427A-2xStrep
Plasmid#131109PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C427A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 427 muta…PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C424A-2xStrep
Plasmid#131110PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C424A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 424 muta…PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHCAG-L2EOP
Plasmid#51783PurposeThe oriP/EBV nuclear antigen (EBNA)-1 elements of Epstein-Barr virus is fused in frame with the luciferase in the herpes simplex virus type-1 amplicon vector, provides prolonged transgene expressionDepositorInsertsLuciferase-2A-EBNA-1
OriP
UseHerpes simplex virus type-1 amplicon vectorTagsExpressionMutationPromoterCAGAvailable sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free GFP-NES
Plasmid#182491PurposeYeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein GFP-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS
GFP-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10 and GAL7Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXPR_220
Plasmid#164560PurposeLentiviral vector that encodes two sgRNA expression cassettes (one constitutive, one inducible via Flp). Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.DepositorInsertsFirst sgRNA cassette
Second sgRNA cassette
UseCRISPR and Lentiviral; Flp/frtTagsExpressionMammalianMutationPromoterHuman U6 and Mouse U6Available sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free NES
Plasmid#182482PurposeYeast integrative plasmid for expressing ERG20 (GAL10 promoter) and nerolidol synthase from Actinidia chinensis (AcNES1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS
NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10 and GAL7Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free ClLIS1 (M)
Plasmid#182484PurposeYeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and limonene synthase from Citrus limon (ClLIS1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS(M)
LS
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10 and GAL7Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free deadFPPS-NES
Plasmid#182492PurposeYeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein deadFPPS-AcNES1 (GAL7 promoter). deadFPPS is ERG20(K197G-K254A), an inactive mutant of ERG20.DepositorInsertsFPPS
deadFPPS-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10 and GAL7Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ccdB-V5
Plasmid#214985PurposeGateway compatible empty vector (transient mammalian expression) with a C-terminal V5 tagDepositorTypeEmpty backboneUseTagsV5ExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only