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Plasmid#39850DepositorInsertCdc20 (CDC20 Human)
UseBaculovirus expressionTagsHisExpressionMutationPromoterAvailable sinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pfast bac-flag Fbxl3
Plasmid#51894PurposeBackbone plasmid is pfastBacHTa from invitrogenDepositorInsertFbxl3 (Fbxl21 Mouse)
UseTagsFLAGExpressionMutationPromoterpolyhedrin promoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-Kozak-rhTRIM5alpha-PFOS
Plasmid#79038PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertrhesus TRIM5alha-PFOS
UseTagsOneSTrEP-FLAGExpressionInsectMutationPromoterpolyhedrinAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-His-Tipin-L195A
Plasmid#23121DepositorInsertTipin (TIPIN Human)
UseTagsHisExpressionInsectMutationChanged Leucine 195 to AlaninePromoterAvailable sinceMarch 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
Golgi-FAST-IRES-iRFP670
Plasmid#216345PurposeFor testing localization of pFAST and iRFP670 proteins to Golgi complexDepositorInsertST6Gal1_pFAST-IRES-Sys1_iRFP670
UseTagsmyc for FAST and iRF670 proteinsExpressionMammalianMutationn/aPromoterCMVAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Peroxisomes-FAST-IRES-iRFP670
Plasmid#216347PurposeFor testing localization of pFAST and iRFP670 proteins to peroxisomesDepositorInsertpFAST_SKL-IRES-iRFP670_PXMP2
UseTagsmyc for FAST protein onlyExpressionMammalianMutationn/aPromoterCMVAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mito-FAST-IRES-iRFP670
Plasmid#216346PurposeFor testing localization of pFAST and iRFP670 proteins to mitochondriaDepositorInsertCox8_pFAST-IRES-Tomm20_iRFP670
UseTagsmyc for FAST and iRF670 proteinsExpressionMammalianMutationn/aPromoterCMVAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-His6-E3(1)-CUbo-TwinStrep
Plasmid#212712PurposeExpression of His6-E3(1)-CUbo-TwinStrep in insect cellsDepositorInsertE3(1)
UseTags6xHis and CUbo-TwinStrepExpressionBacterialMutationPromoterAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
PMP34-mApple-NFAST(low)
Plasmid#214418PurposePeroxisome localization of low-affinity NFAST fused to PMP34-AppleDepositorInsertRspA-N (SLC25A17 )
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
NFAST(low)-mApple-mito
Plasmid#214415PurposeMitochondria localization of low-affinity NFAST fused to mApple-mitoDepositorInsertRspA-N
UseTagsmApple-mitoExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
NFAST(low)-mApple-6xHp
Plasmid#214412PurposeLipid droplet localization of low-affinity NFAST fused to mApple-6xHpDepositorInsertRspA-N
UseTagsmApple-6xHpExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIG-837_NY-ESO-1_TCR_tFAS
Plasmid#207500PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInserttFAS, NY-ESO-1_TCR (FAS Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pFastBac1-Clover4-LUMABS-S1 RBD
Plasmid#183491PurposeTo express the Clover4-LUMABS-S1 RBD sensor for SARS-CoV-2 antibody dection.DepositorInsertClover4-LUMABS-S1 RBD
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2393-Tier1-PhCMV-p2a_fastFT
Plasmid#169530PurposeTier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).DepositorInsertPCMV-driven P2A-fused fluorescent timer, fast maturation time
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2385-Tier1-PhCMV-fastFT
Plasmid#169518PurposeTier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT (PhCMV-fastFT-pA).DepositorInsertPCMV-driven fluorescent timer, fast maturation time
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
HT114_Fsyn_FAS(Cre off)_QuasAr6b_Citrine
Plasmid#178825PurposeNeuronal expression (Cre-off) of an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6b carrying a Citrine expression tagDepositorInsertQuasAr6b_citrine
UseCre/Lox and LentiviralTagscitrineExpressionMammalianMutationPromoterhSynAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFASTbac-HisGST-PTPIP51(36-470)
Plasmid#170531PurposeExpresses Cleavable HisGST-tagged PTPIP51 (36-470) in Sf9DepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 )
UseTagsGST tag and His tagExpressionInsectMutationPromoterPolyhedrinAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-LacI-LAP-HEC1
Plasmid#114033PurposeProduction of baculovirus with LacI-LAP-HEC1DepositorInsertLacI-LAP-HEC1 (NDC80 Human)
UseTagsExpressionInsectMutationnonePromoterCMVAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-ATG101-ATG13 HORMA
Plasmid#99332PurposeMammalian expression of one subcomplex from the Human ULK1 complexDepositorUseTagsGSTExpressionInsectMutationPromoterp10 and pHAvailable sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only