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Showing: 321 - 340 of 4937 results

  13. pSaCas9_GFP

    Plasmid
    #64709
    Purpose
    Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells
    Depositor
    Kiran Musunuru
    Insert
    SaCas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP and 3xFLAG-SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    64709-plasmid-map-sequence-id-322473

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  14. R777-E111 Hs.IRS2

    Plasmid
    #70395
    Purpose
    Gateway ORF clone of codon optimized human IRS2 [NM_003749] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    IRS2 (IRS2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70395-plasmid-map-sequence-id-125844

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  15. R777-E112 Hs.IRS2-nostop

    Plasmid
    #70396
    Purpose
    Gateway ORF clone of codon optimized human IRS2 [NM_003749] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    IRS2 (IRS2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70396-plasmid-map-sequence-id-125845

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  17. pGENT_GW_aco_mScarlet3

    Plasmid
    #225991
    Purpose
    To make gene fusions with Arabidopsis codon-optimized mScarlet3
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    Tags
    mScarlet3
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    225991-plasmid-map-sequence-id-448012

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  18. pFA6a-link-yECFP-Hygro

    Plasmid
    #44647
    Depositor
    Kurt Thorn
    Insert
    yEmCFP
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 9, 2013
    Availability
    Academic Institutions and Nonprofits only
    44647-plasmid-map-sequence-id-61116

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  19. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid
    #182653
    Purpose
    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    Use
    Tags
    HA tag and nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    E55D and Y306A/Y384F (AF) double mutant of Methan…
    Promoter
    CMV and U6
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    182653-plasmid-map-sequence-id-363026

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Showing: 321 - 340 of 4937 results