Addgene

We narrowed to 3,954 results for: chm

Showing: 321 - 340 of 3954 results

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pUB23-M-beta-gal

Plasmid

#8816

Depositor

Alexander Varshavsky

Article

Bachmair et al Science 1986 Oct 10;234(4773):179-86.

Insert

beta-gal

Use

Tags

Ubiquitin

Expression

Yeast

Mutation

M (methionine) at amino terminus of beta-gal

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pEx_MCS-attBtagRFPt

Plasmid

#48876

Purpose

PhiC31 exchange vector with multiple cloning site, exchange of EGFP in landing site with tagRFPt

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al Development. 2013 Sep 18.

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pSB_PhiC31LandingSite

Plasmid

#48875

Purpose

Sleeping beauty transposon with the PhiC31 landing site in a cmlc2::EGFP selection cassette

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al Development. 2013 Sep 18.

Insert

cmlc2::attP-EGFP

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pKDM

Plasmid

#110740

Purpose

Plasmid for the insect cell expression system, two promoters, Multiplication Module M, Tn7 transposition

Depositor

Tim Richmond

Article

Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pUB23-P-beta-gal

Plasmid

#8817

Depositor

Alexander Varshavsky

Article

Bachmair et al Science 1986 Oct 10;234(4773):179-86.

Insert

beta-gal

Use

Tags

Ubiquitin

Expression

Yeast

Mutation

P (proline) at amino terminus of beta-gal

Promoter

Available since

Dec. 28, 2005

Availability

Academic Institutions and Nonprofits only
pUB23-R-beta-gal

Plasmid

#8818

Depositor

Alexander Varshavsky

Article

Bachmair et al Science 1986 Oct 10;234(4773):179-86.

Insert

beta-gal

Use

Tags

Ubiquitin

Expression

Yeast

Mutation

R (arginine) at amino terminus of beta-gal

Promoter

Available since

Dec. 28, 2005

Availability

Academic Institutions and Nonprofits only
pUB23-L-beta-gal

Plasmid

#8820

Depositor

Alexander Varshavsky

Article

Bachmair et al Science 1986 Oct 10;234(4773):179-86.

Insert

beta-gal

Use

Tags

Ubiquitin

Expression

Yeast

Mutation

L (leucine) at amino terminus of beta-gal

Promoter

Available since

Dec. 28, 2005

Availability

Academic Institutions and Nonprofits only
pEx_Hsp70::attBmut

Plasmid

#48878

Purpose

PhiC31 exchange vector with the Hsp70 promoter driving tagRFPt from the landing site. Template for enhancer tests.

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al Development. 2013 Sep 18.

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

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IKK1-ECFP

Plasmid

#111205

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Insert

IKKalpha (CHUK Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

V268I, V426A

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pUB23-K-beta-gal

Plasmid

#8819

Depositor

Alexander Varshavsky

Article

Bachmair et al Science 1986 Oct 10;234(4773):179-86.

Insert

beta-gal

Use

Tags

Ubiquitin

Expression

Yeast

Mutation

K (lysine) at amino terminus of beta-gal

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pGGEV_3_XcmI-LacZ

Plasmid

#49298

Purpose

Golden Gate entry vector at position 3, LacZ

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGEV_3_Linker

Plasmid

#49283

Purpose

Golden Gate entry vector at position 3, small linker insert

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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p3E_GGWDest-

Plasmid

#49316

Purpose

Destination vector for Golden Gate assembly; 3' entry vector for Gateway cloning

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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p5E_GGWDest-

Plasmid

#49318

Purpose

Destination vector for Golden Gate assembly; 5' entry vector for Gateway cloning

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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p5E_GGWDest+

Plasmid

#49319

Purpose

Destination vector for Golden Gate assembly; 5' entry vector for Gateway cloning

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pME_GGWDest+

Plasmid

#49321

Purpose

Destination vector for Golden Gate assembly; middle entry vector for Gateway cloning

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGEV_2_XcmI-LacZ

Plasmid

#49297

Purpose

Golden Gate entry vector at position 2, LacZ

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGDestSC-ATG

Plasmid

#49322

Purpose

Destination vector for Golden Gate assembly; Few restriction sites for subcloning.

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pNHA_SBV_NSs

Plasmid

#208932

Purpose

Expresses N-terminally HA-tagged Schmallenberg orthobunyavirus NSs protein from CMV promoter in mammalian cells

Depositor

Gerald Barry

Insert

NSs

Use

Tags

HA

Expression

Mammalian

Mutation

Deleted 50 bp from 5' end (including start c…

Promoter

CMV

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-Cas9-Exo-Blast-BFP

Plasmid

#196721

Purpose

Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.

Depositor

Bernhard Schmierer

Insert

Cas9-Exo1-T2A-BSD-TagBFP

Use

CRISPR and Lentiviral

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mutation

Promoter

EF1a

Available since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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