Showing: 321 - 340 of 756 results
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Plasmid#132664PurposepRL623-based helper plasmid for conjugations, with RSF1010 mobA geneDepositorInsertMobA
UseTagsExpressionBacterialMutationplease see depositor comments belowPromoterp1,p3AvailabilityAcademic Institutions and Nonprofits only -
pAH-CTX1-rha
Plasmid#129390PurposeDerived from mini-CTX1. An integrase vector that can integrate the rhamnose-inducible promoter system into the chromosome of a host bacterium.DepositorInsertRhamnose-inducible promoter
UseTagsExpressionBacterialMutationN/APromoterPrhaBADAvailabilityAcademic Institutions and Nonprofits only -
pAG4
Plasmid#70115PurposeTn7-based lux reporter for promoter cloning in bacteriaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoternoneAvailabilityAcademic Institutions and Nonprofits only -
pCM157
Plasmid#45863DepositorInsertcre recombinase
UseCre/LoxTagsExpressionBacterialMutationPromoterlacAvailabilityAcademic Institutions and Nonprofits only -
pON.mCherry
Plasmid#84821PurposeConstitutive Expression plasmid with mCherry fluorescent proteinDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterTac PromoterAvailabilityAcademic Institutions and Nonprofits only -
pHSS6hsILMi20
Plasmid#102536PurposeHelper plasmid expressing Minos transposase mRNA (Drosophila hsp70 promoter)DepositorInsertMinos transposase
UseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTD-CTwinStrep
Plasmid#45939Purposeuse to create C-terminal Twin-Strep-tag fusion proteinsDepositorTypeEmpty backboneUseTagsTwin-Strep-tagExpressionBacterialMutationPromoterlacIq-PtrcAvailabilityAcademic Institutions and Nonprofits only -
pAH25-SceI
Plasmid#129389PurposeDerived from pDAI-SceI. Tetracycline resistance removed and replaced with chloramphenicol resistance cassette from pKD3.DepositorInsertChloramphenicol acetyltransferase
UseTagsExpressionBacterialMutationN/APromoterCAT promoterAvailabilityAcademic Institutions and Nonprofits only -
pSCB2-sgRNA
Plasmid#129463PurposeTemplate plasmid for guide sequence insertion via inverse PCR. Derived from pSCrhaB2-gRNA (see paper).DepositorInsertpgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 (SpeI)AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-guideless
Plasmid#129464PurposeDerived from pSCB2-sgRNA. 20 nucleotide binding sequence of sgRNA removed by inverse PCR. Used as a negative control.DepositorInsertGuideless gRNA backbone
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 (SpeI)AvailabilityAcademic Institutions and Nonprofits only -
pUDP123
Plasmid#107269PurposepUDP123 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes OpADE2 and OpNIAD and Spcas9D147Y P411T in O. parapolymorpha (HH-gRNAOpADE2-HDV-linker-HH-gRNAOpNIAD-HDV)DepositorInsertpolycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting OpADE2 and NIAD in O. parapolymorpha
UseTagsExpressionYeastMutationPromoterScTDH3AvailabilityAcademic Institutions and Nonprofits only -
pDAI-SceI
Plasmid#32061PurposeConstruction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonucleaseDepositorInsertI-SceI nuclease
UseTagsExpressionBacterialMutationPromoterPdhfrAvailabilityAcademic Institutions and Nonprofits only -
pMF230
Plasmid#62546PurposeBroad host range plasmid for constitutive expression of the eGFP. Developed for imaging Pseudomonas aeruginosa in biofilms.DepositorInsertGFP mut2
UseBroad host range plasmidTagsExpressionMutationPromotertrcAvailabilityAcademic Institutions and Nonprofits only -
pMiLRTetR(L)
Plasmid#102537PurposePlasmid for testing the activity of the Minos transposon (excision and transposition assays)DepositorInsertMinos transposon carrying TetR gene
UseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAM5505
Plasmid#132664PurposepRL623-based helper plasmid for conjugations, with RSF1010 mobA geneDepositorInsertMobA
UseTagsExpressionBacterialMutationplease see depositor comments belowPromoterp1,p3AvailabilityAcademic Institutions and Nonprofits only -
pAB1
Plasmid#62547Purposebroad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.DepositorInserteGFP
UseBroad host range plasmidTagsExpressionBacterialMutationPromoterpTrcAvailabilityAcademic Institutions and Nonprofits only -
pMP4639
Plasmid#107771PurposeExpression of EYFPDepositorInsertpLacZ-EYFP in pME6010
UseTagspLacZ-EYFPExpressionBacterialMutationPromoterpLacZAvailabilityAcademic Institutions and Nonprofits only -
pMP4657
Plasmid#107773PurposeExpression of EGFPDepositorInsertpLacZ-EGFP in pME6010
UseTagspLacZ-EGFPExpressionBacterialMutationPromoterpLacZAvailabilityAcademic Institutions and Nonprofits only -
pTD-NTwinStrep_Sm
Plasmid#45937Purposeuse to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterlacIq-PtrcAvailabilityAcademic Institutions and Nonprofits only -
pTDpelB-CTwinStrep
Plasmid#45941Purposeuse to create N-terminal PelB and C-terminal Twin-Strep-tagged fusion proteins for periplasmic translocation via PelB signal sequenceDepositorTypeEmpty backboneUseTagsPelB export signal and Twin-Strep-tagExpressionBacterialMutationPromoterlacIq-PtrcAvailabilityAcademic Institutions and Nonprofits only
Showing: 321 - 340 of 756 results