Skip to main content
Addgene
Showing: 321 - 340 of 464 results

  1. pBlastR-5xQUAS-EBFP2-GMRWhite

    Plasmid
    #165921
    Purpose
    Blasticidin resistant 5xQUAS EBFP2 GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Insert
    Selectable QUAS Response Vector
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165921-plasmid-map-sequence-id-328534

    Enlarge

  2. pBlastR-5xQUAS-sfGFP-MiniWhite

    Plasmid
    #165922
    Purpose
    Blasticidin resistant 5xQUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Insert
    Selectable QUAS Response Vector
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165922-plasmid-map-sequence-id-326318

    Enlarge

  3. pBlastR-5xQUAS-EBFP2-MiniWhite

    Plasmid
    #165924
    Purpose
    Blasticidin resistant 5xQUAS EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Insert
    Selectable QUAS Response Vector
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165924-plasmid-map-sequence-id-326303

    Enlarge

  4. pBlastR-5xUAS-EBFP2-MiniWhite

    Plasmid
    #165912
    Purpose
    Blasticidin resistant 5xUAS EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Insert
    Selectable UAS Response Vector
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165912-plasmid-map-sequence-id-331715

    Enlarge

  5. pBlastR-5xUAS-mCherry-GMRWhite

    Plasmid
    #165908
    Purpose
    Blasticidin resistant 5xUAS mCherry GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Insert
    Selectable UAS Response Vector
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    165908-plasmid-map-sequence-id-331714

    Enlarge

  6. pBlastR-5xQUAS-mCherry-GMRWhite

    Plasmid
    #165920
    Purpose
    Blasticidin resistant 5xQUAS mCherry GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Insert
    Selectable QUAS Response Vector
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    165920-plasmid-map-sequence-id-331712

    Enlarge

  7. pBlastR-12xLexAOp-sfGFP-MiniWhite

    Plasmid
    #165916
    Purpose
    Blasticidin resistant 12xLexAOp sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Insert
    Selectable LexAOp Response Vector
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    165916-plasmid-map-sequence-id-331236

    Enlarge

  8. pBlastR-5xQUAS-mCherry-MiniWhite

    Plasmid
    #165923
    Purpose
    Blasticidin resistant 5xQUAS mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Insert
    Selectable QUAS Response Vector
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    165923-plasmid-map-sequence-id-330498

    Enlarge

  10. pSCAR_Cas9-blast_GFP

    Plasmid
    #162074
    Purpose
    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance
    Depositor
    Robert Manguso
    Article
    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2
    Inserts
    Cas9-2a-blasticidin S deaminase
    EGFP
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EF1a and sv40
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162074-plasmid-map-sequence-id-325297

    Enlarge

  11. lenti-sgRNA blast

    Plasmid
    #104993
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.
    Depositor
    Brett Stringer
    Article
    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104993-plasmid-map-sequence-id-342099

    Enlarge

  14. pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196074
    Purpose
    (Empty Backbone) Constitutive CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Promoter
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196074-plasmid-map-sequence-id-389001

    Enlarge

  15. lentiSAMv2

    Plasmid
    #75112
    Purpose
    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    May 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    75112-plasmid-map-sequence-id-305764

    Enlarge

Showing: 321 - 340 of 464 results