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Plasmid#138903PurposeEYFP reporter vector for the ZF43x8-Compact promoterDepositorInsertZF43x8-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x8-CompactAvailable sinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
px459 EQR
Plasmid#101732PurposeExpresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifsDepositorInsertSpCas9 EQR
UseTagsExpressionMammalianMutationVQRPromoterAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-SS-338
Plasmid#68795PurposeSingle construct Sensor pair 1, ZF9 Op x6 -- GFP, with mCherry markerDepositorInsertsC-Terminal Sensor 1 V1
mCherry
ZF9 Op x6
EGFP
N-Terminal Sensor 1 V2
UseTagsIRES, NLS, flag, and nlsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPD289 ZF43x5-Compact_EYFP
Plasmid#138900PurposeEYFP reporter vector for the ZF43x5-Compact promoterDepositorInsertZF43x5-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x5-CompactAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD287 ZF43x3-Compact_EYFP
Plasmid#138898PurposeEYFP reporter vector for the ZF43x3-Compact promoterDepositorInsertZF43x3-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x3-CompactAvailable sinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD286 ZF43x2-Compact_EYFP
Plasmid#138897PurposeEYFP reporter vector for the ZF43x2-Compact promoterDepositorInsertZF43x2-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x2-CompactAvailable sinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD285 ZF43x1-Compact_EYFP
Plasmid#138896PurposeEYFP reporter vector for the ZF43x1-Compact promoterDepositorInsertZF43x1-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x1-CompactAvailable sinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2
Plasmid#165856PurposeUniversal Part domesticator vector v2 used for integration (domestication) of new DNA elements in the GB2.0 cloning system uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
p8389 LentiCRISPRv2 Neo sgNT-1
Plasmid#221649PurposeExpression of non-targeting control sgRNADepositorInsertnon-targeting sgNT-1
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p8390 LentiCRISPRv2 Neo sgNT-2
Plasmid#221650PurposeExpression of non-targeting control sgRNADepositorInsertnon-targeting sgNT-2
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVD2
Plasmid#165857Purposeuniversal Vector Domesticator vector v2 used in the integration (domestication) of new vector backbone parts in the GB2.0 cloning system. Uses blue-white screening methodDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
px459 VQR
Plasmid#101715PurposesgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)DepositorInsertSpCas9 VQR
UseTags3xFLAG-NLS and NLSExpressionMammalianMutationVQR (D1135V, R1335Q and T1337R)PromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
YSBE 508 : pNEO_GFP
Plasmid#92081PurposePlasmid for tagging proteins in Cryptococcus neoformans; GFP tag, Neo selectionDepositorInsertGFP
Usetagging proteins in cryptococcus neoformanTagsExpressionMutationPromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPD1028 ZF43x6-Compact(SV40Min)_EYFP
Plasmid#138912PurposeEYFP reporter vector for the ZF43x6-Compact(overlapping ZF37) promoter with a SV40 minimal promoterDepositorInsertZF43x6-Compact(SV40min)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x6-Compact(SV40min)Available sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
MCP-ADAR2-DD
Plasmid#170124PurposeLentiviral vector carrying the MCP-ADAR2 with a nuclear export signalDepositorInsertMCP-ADAR2-DD-NES
UseLentiviralTagsExpressionMammalianMutationPromoterEf1a core promoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
YSBE 516 : pNEO_4xFLAG
Plasmid#92082PurposePlasmid for tagging proteins in Cryptococcus neoformans; 4xFLAG tag, Neo selectionDepositorInsert4xFlag
Usetagging proteins in cryptococcus neoformanTagsExpressionMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
YSBE 517 : pNEO_6xHA
Plasmid#92083PurposePlasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, Neo selectionDepositorInsert6xHA
Usetagging proteins in cryptococcus neoformanTagsExpressionMutationPromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
YSBE 524 : pNEO_mCherry
Plasmid#92084PurposePlasmid for tagging proteins in Cryptococcus neoformans; mCherry tag, Neo selectionDepositorInsertmCherry
Usetagging proteins in cryptococcus neoformanTagsExpressionMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
px459 VRER
Plasmid#101716PurposesgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)DepositorInsertSpCas9 VRER
UseTags3xFLAG-NLS and NLSExpressionMammalianMutationVRER (D1135V, G1218R, R1335E and T1337R)PromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
YSBE 610 : pHYG_6xHA
Plasmid#92091PurposePlasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, HYG selectionDepositorInsert6xHA
Usetagging proteins in cryptococcus neoformanTagsExpressionMutationPromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only