Skip to main content
Addgene
Showing: 321 - 340 of 548 results

  4. pGST-HsSyx-375-792

    Plasmid
    #179292
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + GST + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Use
    Tags
    His6 + GST + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    residues 375-792 from accession number NP_0010361…
    Promoter
    T7
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179292-plasmid-map-sequence-id-354918

    Enlarge

  5. pSUMO-HsSyx3

    Plasmid
    #179288
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + SUMO + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Use
    Tags
    His6 + SUMO + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    residues 393-792 from accession number NP_0010361…
    Promoter
    T7
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179288-plasmid-map-sequence-id-354924

    Enlarge

  6. pJK573

    Plasmid
    #72456
    Purpose
    Produces Yersinia pestis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tYpPurE1), synthetic gene
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    synthetic gene with ~57 silent substitutions
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72456-plasmid-map-sequence-id-133910

    Enlarge

  13. pJK569

    Plasmid
    #72455
    Purpose
    Produces Franciscella tularensis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tFtPurE1), synthetic gene
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    synthetic gene with ~51 silent substitutions
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72455-plasmid-map-sequence-id-133906

    Enlarge

  16. pMBP-HsSyx3

    Plasmid
    #179287
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Use
    Tags
    His6 + mature maltose binding protein + TEV prote…
    Expression
    Bacterial
    Mutation
    residues 393-792 from accession number NP_0010361…
    Promoter
    T7
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179287-plasmid-map-sequence-id-354925

    Enlarge

  17. pMBP-HsSyx-375-792

    Plasmid
    #179291
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Use
    Tags
    His6 + mature maltose binding protein + TEV prote…
    Expression
    Bacterial
    Mutation
    residues 375-792 from accession number NP_0010361…
    Promoter
    T7
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179291-plasmid-map-sequence-id-354915

    Enlarge

  18. MSP972

    Plasmid
    #98806
    Purpose
    Expression Vector
    Depositor
    David Liu
    Article
    Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1
    Insert
    pET28 MBP-ENLYFQS-TEV T17S, N68D, E107D, D127A, F132L, S135F, F162S, K229E
    Use
    Tags
    Expression
    Bacterial
    Mutation
    T17S, N68D, E107D, D127A, F132L, S135F, F162S, K2…
    Promoter
    Available since
    Oct. 20, 2017
    Availability
    Academic Institutions and Nonprofits only

  19. pMLLAC-J72041

    Plasmid
    #26186
    Depositor
    Christopher Anderson
    Insert
    Tev Protease Cleavage site
    Use
    Synthetic Biology; 2ab assembly vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 3, 2011
    Availability
    Academic Institutions and Nonprofits only
    26186-plasmid-map-sequence-id-12307

    Enlarge

  20. pCsy_complex

    Plasmid
    #89232
    Purpose
    Plasmid contains the genes Csy1, Csy2, Csy3, Csy4, cloned as a polycistronic operon from bacterium Pseudomonas aeruginosa PA14. Csy3 includes an N-terminal 6his tag and TEV protease cleavage site
    Depositor
    Blake Wiedenheft
    Article
    Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:
    Inserts
    Csy1
    Csy2
    Csy3
    Csy4
    Use
    Tags
    6xHis-TEV protease
    Expression
    Mutation
    Promoter
    Available since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    89232-plasmid-map-sequence-id-177172

    Enlarge

Showing: 321 - 340 of 548 results