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Plasmid#59969PurposeBacterial Expression GST-WDR5DepositorInsertWDR5 (WDR5 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MBP-(CBX1)3-WT
Plasmid#180212PurposeBacterial expression of engineered CBX1 fusions with enhanced binding to H3K9meDepositorInsertCBX1 chromobox protein 1 x3 (CBX1 Human)
UseTags6xhis-tag and MBPExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEM-HSPA5P
Plasmid#44150Purposea fragment containing 429 bp of the human HSPA5 promoter (-282 to +147)DepositorInsertHSPA5 promoter (HSPA5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 MYC dLZ
Plasmid#176048PurposeExpresses Myc Mutant (delta leucine zipper) in Mammalian cellsDepositorInsertMyc
UseTagsExpressionMammalianMutationdeleted amino acids 414-433PromoterCMVAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
ALARM NMR
Plasmid#90209PurposeExpresses the human La antigen for use in ALARM NMRDepositorInsertLa antigen (SSB Human)
UseTags6xHisExpressionBacterialMutationcontains amino acids 100-324PromoterT7 promoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
mIFP-H2B-6
Plasmid#56223PurposeLocalization: Nucleus/Histones, Excitation: 683, Emission: 704DepositorInsertH2B (H2BC11 Human)
UseTagsmIFPExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSG5-FLAG-mEKLF
Plasmid#67833PurposeExpression of FL-EKLF driven by SV40 promoterDepositorInsertEKLF (Klf1 Mouse)
UseTagsFLAGExpressionMammalianMutationFull-length mEKLF is aa 20-376; amino acid 19 is …PromoterSV40Available sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
His6tev CBP BCHZDeltaAL
Plasmid#99551PurposeBacterial expression of His tagged mouse CBP HAT Brd-ZZ(1079-1751) 1557-1617 replaced by SGGSGDepositorInsertmouse CBP BRD-ZZ domains (1079-1751) DeltaAL (Crebbp Mouse)
UseTagsHis6tevExpressionBacterialMutationreplaced 1557-1618 with SGGSGPromoterT7Available sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-TRR-C421-Y2404F
Plasmid#182846Purposemutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG inductionDepositorInsertTRR 2011-2431 (trr Fly)
UseTagsExpressionBacterialMutationmutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…PromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pASK/3xMBT
Plasmid#28234DepositorInsertl(3)mbt-like 1 (Drosophila) (L3MBTL1 Human)
UseTagsHisExpressionBacterialMutationTruncation expressing only aa 252-606 (MBT repeat…PromoterAvailable sinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
mCherry-miniSOG-H2B-C-10
Plasmid#55090PurposeLocalization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertH2B (H2BC11 Human)
UseTagsmCherry-miniSOGExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBABE hygro MEN1 L22R
Plasmid#11021DepositorInsertMEN1 L22R (MEN1 Human)
UseRetroviralTagsExpressionMammalianMutationL22RPromoterAvailable sinceDec. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
Flag-TRIM24-C840W shRescue
Plasmid#28137DepositorInsertTRIM24 (TRIM24 Human)
UseTagsFLAGX2ExpressionMammalianMutationC840Wmutated at aa 308 and 309 for shRNA rescue; …PromoterAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBABE hygro MEN1 T344R
Plasmid#11019DepositorInsertMEN1 T344R (MEN1 Human)
UseRetroviralTagsExpressionMammalianMutationT344RPromoterAvailable sinceDec. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pBABE hygro MEN1 W436R
Plasmid#11018DepositorInsertMEN1 W436R (MEN1 Human)
UseRetroviralTagsExpressionMammalianMutationW436RPromoterAvailable sinceDec. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pBABE hygro MEN1 P12L
Plasmid#11020DepositorInsertMEN1 P12L (MEN1 Human)
UseRetroviralTagsExpressionMammalianMutationP12LPromoterAvailable sinceDec. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pBABE hygro MEN1 A309P
Plasmid#11023DepositorInsertMEN1 A309P (MEN1 Human)
UseRetroviralTagsExpressionMammalianMutationA309PPromoterAvailable sinceDec. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pENTR-actin-V159N
Plasmid#118383PurposeGateway compatible donor vector with actin-V159N.DepositorInsertactin beta (ACTB Human)
UseDonor-plasmidTagsExpressionMutationV159NPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-actin-G168D,Y169D
Plasmid#118384PurposeGateway compatible donor vector with actin-G168D,Y169D.DepositorInsertactin beta (ACTB Human)
UseDonor-plasmidTagsExpressionMutationG168D,Y169DPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWZLneo Flag hDPY30
Plasmid#15555DepositorInsertDPY30 (DPY30 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
macroH2A1.1-GFP (pc2188)
Plasmid#223707PurposeExpression of macroH2A1.1 tagged N-terminal to GFPDepositorInsertmacroH2A1.1-GFP (Macroh2a1 Rat)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
macroH2A1.2-GFP (pc2189)
Plasmid#223708PurposeExpression of macroH2A1.2 tagged N-terminal to GFPDepositorInsertmacroH2A1.2-GFP (Macroh2a1 Rat)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
hzHDAC10
Plasmid#225542PurposeExpresses histone deacetylase 10 (HDAC10) humanized catalytic domain, also known as hzHDAC10, in Escherichia coli cells.DepositorInsertHistone Deacetylase 10 humanized catalytic domain (hdac10 Zebrafish)
UseTagsHis6-MBP tag with cleavable TEV siteExpressionBacterialMutationMutated A24E and D94APromoterT7Available sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEG302-HD2C-ZF
Plasmid#200917PurposeExpress HD2C-ZF108 in Arabidopsis target FWA geneDepositorInsertHD2C (HD2C Mustard Weed)
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-TRR-C421-G2304D
Plasmid#182845Purposemutation G2304D (GGC/GAC), PCR product coding C-terminal residues 2011-2431 of TRR was inserted into modified pGEX-2T by Nde I-Nsi I sitesDepositorInsertTRR 2011-2431 (trr Fly)
UseTagsGSTExpressionBacterialMutationmutation G2304D (GGC/GAC)PromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-CBP(1-287)
Plasmid#182839PurposeCBP(1-287) was inserted into pGEX-2T with NdeI and NsiI sites. pGEX-2T-CBP(1-287) was used in the GST pull-down assay described in our paper (Tie et al. 2012 MCB, Tie et al 2014 Development)DepositorInsertCBP1-287
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET11-PC(75-228)-H6
Plasmid#183779PurposeExpress PC(75-228)-H6 in E. coli. PC(75-228)-H6 was used for GST-CBP pull-down assays.DepositorInsertPolycomb (Pc Fly)
UseTags6 His Tag at the C-terminusExpressionBacterialMutationPromoterT7Available sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET11-PC(75-390)-H6
Plasmid#183781PurposeExpress PC(75-390)-H6 in E. coli. PC(75-390)-H6 was used for GST-CBP pull-down assaysDepositorInsertPolycomb (w Fly)
UseTags6 His tag at the C-terminusExpressionBacterialMutation6 His tag at the C-terminusPromoterT7Available sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PC(1-86)-H6
Plasmid#183780PurposeExpress PC(1-86)-H6 in E. coli. PC(1-86)-H6 was used for GST-CBP pull-down assaysDepositorInsertPolycomb (Pc Fly)
UseTags6 His Tag at the C-terminusExpressionBacterialMutationPromoterT7Available sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-FLAG-CBPΔ(N+HAT)
Plasmid#182841PurposeAn EcoRV fragment (encoding residues 1566-2142) was removed from pMT-FLAG-CBPΔN. Expression in S2 cells by CuSO4 induction.DepositorInsertCBP (w Fly)
UseTagsFLAG tagExpressionInsectMutationdletion of residues 1566-2142 from CBP1476-3222PromoterMTAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 MYC dBR
Plasmid#176046PurposeExpresses Myc Mutant (delta basic region) in Mammalian cellsDepositorInsertMyc
UseTagsExpressionMammalianMutationdeleted amino acids 355-369PromoterCMVAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-KAT14-547-782
Plasmid#118376PurposeGateway compatible donor vector with KAT14-547-782 truncation.DepositorInsertKAT14 (KAT14 Human)
UseDonor-plasmidTagsExpressionMutationTruncation (aa 547-782)PromoterAvailable sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-KAT14-1-546
Plasmid#118377PurposeGateway compatible donor vector with KAT14-1-546 truncation.DepositorInsertKAT14 (KAT14 Human)
UseDonor-plasmidTagsExpressionMutationTruncation (aa 1-546)PromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
RCH1:GA2ox2-GFP
Plasmid#108567PurposeArabidopsis transformation, expresses GA2ox2 with RCH1 promoter to study expression pattern in rootsDepositorInsertGA2ox2 (GA2OX2 Mustard Weed)
UseTagsExpressionPlantMutationPromoterRCH1Available sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGA2ox2:GUS
Plasmid#108564PurposeArabidopsis transformation, expresses GA2ox2 promoter/GUS fusion to study expression pattern in rootsDepositorInsertGA2ox2 promoter (GA2OX2 Mustard Weed)
UseTagsGUSExpressionPlantMutationPromoterGA2ox2Available sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry-miniSOG-H2B-C-10
Plasmid#57789PurposeLocalization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertH2B (H2BC11 Human)
UseTagsPA-mCherry-miniSOGExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-H3.3-G34V
Plasmid#209433PurposeLentiviral packagable plasmid expressing FLAGGED human histone mutant H3.3-G34V with BFPDepositorInsertH3.3-G34V
UseTagsExpressionMammalianMutationG34VPromoterAvailable sinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2-H3.1-WT-BFP
Plasmid#209437PurposeSB-transposable plasmid expressing FLAGGED human histone mutant H3.1-Wt with BFPDepositorInsertH3.1-WT
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-H3.3K27M-FLAG-BFP
Plasmid#209434PurposeLentiviral packagable plasmid expressing FLAGGED human histone mutant H3.3-K27M with BFPDepositorInsertH3.3-K27M
UseTagsExpressionMammalianMutationK27MPromoterAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ H2B-mTagBFP2
Plasmid#99267PurposeContains a fusion of human histone H2B to fluorescent protein mTagBFP2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.DepositorInsertmTagBFP2-3XHA
UseTagsH2BExpressionMammalianMutationPromoterSP6Available sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
FU-H2B-GFP-IRES-Puro
Plasmid#69550PurposeLentiviral, selectable fluorescent GFP tagging of nuclei.DepositorInsertHistone 2B - green fluorescent protein fusion
UseLentiviralTagsGFP fusionExpressionMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ H2B-Katushka2
Plasmid#99266PurposeContains a fusion of human histone H2B to fluorescent protein Katushka2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.DepositorInsertKatushka2-3XHA
UseTagsH2BExpressionMammalianMutationPromoterSP6Available sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKT-H3.3-K27M-katushka
Plasmid#209443PurposeSB-transposable plasmid expressing a mutant histone H3.3-K27M in tandem with Katushka (RFP)DepositorInsertH3.3-K27M
UseTagsExpressionMammalianMutationK27MPromoterAvailable sinceJan. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ H2B-mCerulean
Plasmid#99268PurposeContains a fusion of human histone H2B to fluorescent protein mCerulean in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcriptionDepositorInsertmCerulean-3XFLAG
UseTagsH2BExpressionMammalianMutationPromoterSP6Available sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ H2B-mRuby2
Plasmid#99270PurposeContains a fusion of human histone H2B to fluorescent protein mRuby2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.DepositorInsertmRuby2-3XHA
UseTagsH2BExpressionMammalianMutationPromoterSP6Available sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ H2B-mEmerald
Plasmid#99269PurposeContains a fusion of human histone H2B to fluorescent protein mEmerald in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.DepositorInsertmEmerald
UseTagsH2BExpressionMammalianMutationPromoterSP6Available sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
H2B CFP
Plasmid#198059PurposemCerulean fused to human H2B proteinDepositorInsertH2B
UseSynthetic Biology; Sea urchin, mammal, zebrafishTagsmCeruleanExpressionMutationPromotersp6Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBac[3xP3-gTc'v;Tc'puP-Tc'H2Av_EGFP]
Plasmid#86455PurposeNuclear reporter (histone-tagged EGFP) construct with Tribolium specific polyubiquitin promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker)DepositorInsert3xP3-gTc'v;Tc'puP-Tc'H2Av_EGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKT-H3.3-G34R-IRES-katushka
Plasmid#209428PurposeSB-transposable plasmid expressing the mutant histone H3.3-G34R with RFPDepositorInsertH3.3-G34R
UseTagsExpressionMammalianMutationG34RPromoterAvailable sinceJan. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
2NVR
Plasmid#51340PurposeCatalytic domain of human histone deacetylase HDAC7DepositorInsertHDAC7
UseTagsHisExpressionBacterialMutationTruncation: See CommentsPromoterT7Available sinceFeb. 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits