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Plasmid#85736PurposeExpresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coliDepositorInsertGas vesicle protein C (truncated and his-tagged)
UseTagsHis-tagExpressionBacterialMutationDeletion of N and C terminal regions, addition of…PromoterT7Available sinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
HOXC8 (murine) HIS-tag pET
Plasmid#8567DepositorInsertHOXC8 (Hoxc8 Mouse)
UseTagsHis and T7ExpressionBacterialMutationmakes protein by WesternPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
HOXC6 (human) HIS-tag pET
Plasmid#8565DepositorInsertHOXC6 (HOXC6 Human)
UseTagsHis and T7ExpressionBacterialMutationThis protein starts at an internal Met initially …PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-SNAP-tag_cx
Plasmid#167273PurposeOverexpression of crystallizing SNAP-tag variant (P179R) in E. coliDepositorInsertSNAP-tag
UseTagsHis x10ExpressionBacterialMutationP179RPromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-3 (C50 1.4 uM)
Plasmid#117910PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 1.4 uMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R44A)
UseTagsHis-tag and Strep-tagExpressionBacterialMutationR44A on eDHFRPromoterT7Available sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (925-953)
Plasmid#73744PurposeExpression of GFP-PP-RPN2 (925-953) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
UseTags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 932-953 of RPN2PromoterT7Available sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-CBM-CohI
Plasmid#58709PurposeProbing molecule to perform single molecule force spectroscopy experimentsDepositorInsertCBM-CohI
UseTagsHIS, HRV 3C, and ybbRExpressionBacterialMutationchanged Alanine 2 to Cysteine in CBM domainPromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GgVcl 884-1066 (aka Vt)
Plasmid#46176DepositorInsertVcl 884-1066 (VCL Chicken)
UseTagsHisExpressionBacterialMutationaa 884-1066 (tail domain)PromoterT7Available sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
HOXB13 (human) HIS-tag pET
Plasmid#8543DepositorInsertHOXB13 (HOXB13 Human)
UseTagsHis and T7ExpressionBacterialMutationmakes protein by Western and by transcription/tra…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-RABIF
Plasmid#135556PurposeExpress human RABIF in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertRABIF (RABIF Human)
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationPromoterT7 promoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
HOXD12 (murine) HIS-tag pET
Plasmid#8553DepositorInsertHOXD12 (Hoxd12 Mouse)
UseTagsHis and T7ExpressionBacterialMutationFusion sequence is: ggatccATGPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
p139 pETF-GST RanBP10 L301I
Plasmid#11092DepositorInsertRanBP10 (Ranbp10 Mouse)
UseTagsGSTExpressionBacterialMutationL301I; see depositor comments belowPromoterAvailable sinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
HOXD4 (murine) HIS-tag pET
Plasmid#8548DepositorInsertHOXD4 (Hoxd4 Mouse)
UseTagsHis and T7ExpressionBacterialMutationmakes protein by Western and by in vitro transcri…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pET15b/GgVcl 884-1066-ECFP
Plasmid#46182DepositorInsertVcl 884-1066 (VCL Chicken)
UseTagsECFP and HisExpressionBacterialMutationaa 884-1066 (tail domain)PromoterT7Available sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
p230-pOsAct1::hpt-pZmUbi::BdPetC
Plasmid#129649PurposeRieske FeS overexpressionDepositorInsertCDS of PetC gene encoding for the Rieske FeS subunit
UseTagsExpressionPlantMutationCodon modified for Golden GatePromoterpZmUbiAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (940-953)
Plasmid#73746PurposeExpression of GFP-PP-RPN2 (940-953) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
UseTags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 940-953 of RPN2PromoterT7Available sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (918-932)
Plasmid#73748PurposeExpression of GFP-PP-RPN2 (918-932) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
UseTags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 918-932 of RPN2PromoterT7Available sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
HOXC5 (human) HIS-tag pET
Plasmid#8563DepositorInsertHOXC5 (HOXC5 Human)
UseTagsHis and T7ExpressionBacterialMutationStarts at Amino Acid #2 (Ser); makes protein by W…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GgVcl V1-258 (aka VD1)
Plasmid#46173DepositorInsertVcl 1-258 (VCL Chicken)
UseTagsHisExpressionBacterialMutationaa 1-258 (VD1)PromoterT7Available sinceJuly 22, 2013AvailabilityAcademic Institutions and Nonprofits only