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Plasmid#123684PurposeEncodes ConLS and the human A4 insulator as a Type 1 part to be used in the MTK systemDepositorInsertConnector LS-A4 insulator
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3
Plasmid#65451PurposepILGFP3 was designed to test the promoter activity using the yeast enhanced green fluorescent protein (yEGFP) as reporter.DepositorTypeEmpty backboneUseTagsyEGFP reporterExpressionYeastMutationPromoterAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pITGFP3
Plasmid#65452PurposepITGFP3 was designed to test the promoter activity using the destabilized yeast enhanced green fluorescent protein (yEGFP-CLN2PEST) as reporter.DepositorTypeEmpty backboneUseTagsyEGFP-CLN2PEST reporterExpressionYeastMutationPromoterAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET20b-GpNLuc-6xHis
Plasmid#135932PurposeExpresses the GpNLuc LumiFluor in bacterial cells.DepositorInsertGpNLuc LumiFluor
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK1_024
Plasmid#123686PurposeEncodes ConL2 and human C3 insulator as a Type 1 part to be used in the MTK systemDepositorInsertConnector L2-C3 insulator
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-11
Plasmid#160711PurposeFor C-terminal tags with Trichoderma optimised Synthetic eGFP bordered by BsaI restriction sitesDepositorInsertTrichoderma optimised Synthetic eGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
MTK1_023
Plasmid#123685PurposeEncodes ConL1 and human B1 insulator as a Type 1 part to be used in the MTK systemDepositorInsertConnector L1- B1 insulator
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-5
Plasmid#160705PurposeTrichoderma optimised Synthetic eGFP bordered by BsaI restriction sitesDepositorInsertTrichoderma optimised Synthetic eGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
MTK2_008
Plasmid#123703PurposeEncodes human pUbc as a Type 2 part to be used in the MTK systemDepositorInsertphUbC
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLIK TT 3xFLAG Luciferase neo
Plasmid#98392PurposeLentiviral vector for inducible expression of Luciferase with 3xFLAG N-terminal tagDepositorInsertLuciferase
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
D. vulgaris Cas7/pHMGWA
Plasmid#81184PurposeExpresses Desulfovibrio vulgaris Cas7 in E. coli with TEV-cleavable N-terminal His-MBP tagDepositorInsertCas7
UseCRISPRTags6xHis - MBP - TEV protease siteExpressionBacterialMutationPromoterT7Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
MTK5_008
Plasmid#123852PurposeEncodes ConRE' and the chicken hypersensitivity site 4 insulator as a Type 5 part to be used in the MTK systemDepositorInsertConnector RE'-2xHS4
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
CS2-GFP-Ben
Plasmid#47966PurposeExpression of fly BenDepositorInsertBendless (ben Fly)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
MTK1_028
Plasmid#123690PurposeEncodes ConL6 and human F1 insulator as a Type 1 part to be used in the MTK systemDepositorInsertConnector L6-F1 insulator
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK1_029
Plasmid#123691PurposeEncodes ConL7 and human B2 insulator as a Type 1 part to be used in the MTK systemDepositorInsertConnector L7-B2 insulator
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMB38-HF380
Plasmid#37332DepositorInsertCytoplasmic Dynein Heavy Chain (dhcA Dictyostelium discoideum)
UseDictyostelium expressionTagsHis6 and FLAG tagExpressionMutationdeleted amino acids 1-1387PromoterTRE-PminAvailable sinceAug. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
D. vulgaris Cas5c/pHMGWA
Plasmid#81182PurposeExpresses Desulfovibrio vulgaris Cas5c in E. coli with TEV-cleavable N-terminal His-MBP tagDepositorInsertCas5c
UseCRISPRTags6xHis - MBP - TEV protease siteExpressionBacterialMutationPromoterT7Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
D. vulgaris Cas8c/pHMGWA
Plasmid#81183PurposeExpresses Desulfovibrio vulgaris Cas8c in E. coli with TEV-cleavable N-terminal His-MBP tagDepositorInsertCas8c
UseCRISPRTags6xHis - MBP - TEV protease siteExpressionBacterialMutationPromoterT7Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
MTK6_016
Plasmid#124058PurposeEncodes the extended Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) to be used in the MTK systemDepositorInsertWPRE (extended)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK2_024
Plasmid#123716PurposeEncodes crippled mouse pPGK1 as a Type 2 part to be used in the MTK systemDepositorInsertpPGK1c
UseTagsExpressionMammalianMutationCrippled Kozac sequencePromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only