Addgene

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AAVS1-tdTomato targeting vector (compatible with CAGGS-AsCpf1-2A-GFP-U6-AAVS1-sgRNA)

Plasmid

#194728

Purpose

AAVS1-tdTomato targeting vector, compartible with Cpf1

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

tdTomato

Use

CRISPR; Knockin targeting plasmid

Tags

Expression

Mutation

Promoter

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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CAGGS-AsCpf1-2A-GFP-U6-AAVS1-sgRNA

Plasmid

#194723

Purpose

based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA that target hAAVS1

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

AsCpf1 (AAVS1 Acidaminococcus sp.)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pED14xY-1

Plasmid

#134807

Purpose

IPTG inducible expression of Y-TAGA qtRNA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

Y-TAGA qtRNA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBP

Plasmid

#72947

Purpose

Backbone vector with internal BsmBI removed by site-directed mutagenesis

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pRAVE-nLacZ (CC#111)

Plasmid

#15135

Depositor

Connie Cepko

Article

Peters et al Dev Biol. 2002 Nov 1. 251(1):59-73.

Insert

nLacZ

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pnEA-NpHG_N

Plasmid

#147033

Purpose

Bacterial Expression vector

Depositor

Elisa Izaurralde

Article

Diebold et al J Struct Biol. 2011 Aug;175(2):178-88. d

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pnEK-NvS_M

Plasmid

#146926

Purpose

Bacterial Expression vector

Depositor

Elisa Izaurralde

Article

Diebold et al J Struct Biol. 2011 Aug;175(2):178-88. d

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pISH-OMP

Plasmid

#15928

Depositor

Peter Mombaerts

Article

Rodriguez et al Nat Neurosci. 2002 Feb . 5(2):134-40.

Insert

OMP (Omp Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only
pQLinkHD_mTFP1

Plasmid

#118858

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

mTFP1

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pJSH-B14

Plasmid

#20145

Depositor

Glen Hartman

Article

Haudenshield et al Plant Dis. 2011 Mar;95(3):343-352. doi:

Insert

Artificial sequence

Use

Cre/Lox

Tags

Expression

Mutation

n/a

Promoter

Available since

Feb. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pBNJ28.2

Plasmid

#66874

Purpose

For transposon tagging with YFP

Depositor

Thomas Hughes

Article

Sheridan et al BMC Biotechnol. 2004 Aug 18;4:17.

Insert

eYFP in a transposon

Use

Source for a tn7 transposon

Tags

eYFP

Expression

Mutation

Promoter

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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6x_a1gRNAOp_pCYC1m_yeBFP2

Plasmid

#64392

Purpose

encodes six a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

yeast enhanced BFP2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pICH82094

Plasmid

#48074

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pBW700_pCAG-pDest-U1-UX

Plasmid

#87578

Purpose

pCAG destination vector (U1-UX)

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBNJ26.3

Plasmid

#66876

Purpose

For CFP tagging via transposon insertion.

Depositor

Thomas Hughes

Article

Sheridan et al BMC Biotechnol. 2004 Aug 18;4:17.

Insert

eCFP in a transposon

Use

Source for a tn7 transposon

Tags

eCFP

Expression

Mutation

Promoter

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pISH-V1rb1

Plasmid

#16010

Depositor

Peter Mombaerts

Article

Rodriguez et al Nat Neurosci. 2002 Feb . 5(2):134-40.

Insert

V1rb1 (Vmn1r50 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
pU6-EF-gAAVS1

Plasmid

#112262

Purpose

Express g-AAVS1

Depositor

Zhili Rong

Article

Ma et al FEBS Lett. 2018 Jun 5. doi: 10.1002/1873

Insert

g-AAVS1

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2+8CmCherry-ABCC1

Plasmid

#40587

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Insert

ABCC1

Use

Sea urchin, zebrafish, xenopus

Tags

mCherry

Expression

Mutation

Promoter

SP6

Available since

Jan. 16, 2013

Availability

Academic Institutions and Nonprofits only
pBW702_pCAG-PV2

Plasmid

#87562

Purpose

Simple PV2

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBW701_pCAG-PV1

Plasmid

#87561

Purpose

Simple PV1

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge