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pSLQ7501 pHR (hU6-crNT-EFS-PuroR-WPRE)

Plasmid

#214876

Purpose

Lentiviral vector encoding RfxCas13d nontargeting control guide

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crNT-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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Cav3-YFP

Plasmid

#69507

Purpose

Expression of fluorescently tagged Caveolin-3 in mammalian cells

Depositor

Rob Parton

Article

Pol et al Mol Biol Cell. 2004 Jan;15(1):99-110. Ep

Insert

Cav3 (Cav3 Mouse)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only
ZFP36L2

Plasmid

#156149

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

ZFP36L2 (ZFP36L2 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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ACVR1B-bio-His

Plasmid

#51640

Purpose

Expresses full-length Activin receptor type 1B precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

ACVR1B (ACVR1B Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-DsRed_IRES_MAPT:EGFP

Plasmid

#92196

Purpose

Lentiviral overexpression vector to make stable bicistronic cell line for Tau screen

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

DsRed-IRES-MAPT-EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-Cx43-HA

Plasmid

#49851

Purpose

Encodes human connexin 43 with a C-terminal HA epitope fusion tag

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Contains several wobble mutations to confer resis…

Promoter

CMV

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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PPP1CA

Plasmid

#155843

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PPP1CA (PPP1CA Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pYFP-ErbB2

Plasmid

#66948

Purpose

Mammalian expression of rat ErbB2 tagged with YFP

Depositor

Mads Lerdrup, Bo van_Deurs

Article

Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

Insert

ErbB2 (Erbb2 Rat)

Use

Tags

6xHis, V5 Tag, and YFP

Expression

Mammalian

Mutation

(See depositor comment below on mutations A24T an…

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pCMV6-Neo-ISG15-LRAA

Plasmid

#80405

Purpose

overexpression of nonconjugative ISG15

Depositor

Isidoro Martinez

Article

Gonzalez-Sanz et al J Virol. 2016 Jan 13;90(7):3428-38. doi:

Insert

ISG15 (ISG15 Human)

Use

Tags

Expression

Mammalian

Mutation

nonconjugative ISG15, LRAA

Promoter

CMV

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV2-SynTetOff-palGFP

Plasmid

#190232

Purpose

AAV vector, high-level expression of EGFP with a palmitoylation signal derived from GAP-43 N-terminus (palGFP) in neuronal cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Use

AAV

Tags

palmitoylation signal sequence

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-MAP1B

Plasmid

#44396

Depositor

Phillip Gordon-Weeks

Article

Scales et al J Cell Sci. 2009 Jul 15;122(Pt 14):2424-

Insert

MAP1B (Map1b Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 21, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-gamma-tubulin

Plasmid

#87956

Purpose

Expresses human gamma-tubulin with a GFP-tag on the C-terminal of the protein

Depositor

Ana Carrera, Maria Alvarado-Kristensson

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

gamma-tubulin 1 (TUBG1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

The protein is a sh-gamma-tubulin resistant gene …

Promoter

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-EZH2

Plasmid

#28060

Depositor

Mien-Chie Hung

Article

Wei et al Nat Cell Biol. 2011 Jan . 13(1):87-94.

Insert

EZH2 (EZH2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-Intron-CterFlag-MePCE

Plasmid

#113549

Purpose

Mammalian expression plasmid that can be used with the Flp-In T-REx system.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe EGFR(L858R/T790M)

Plasmid

#32073

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

L858R/T790M

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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TPC1-mCherry

Plasmid

#135182

Purpose

Expresses TPC1 with C-terminus mCherry tag in mammalian cells

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TPC1 (Tpcn1 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pTET2-lucCP-3MS2

Plasmid

#198351

Purpose

Part of Firefly reporter system, encodes Firefly luciferase with MS2 stem loops in the 3'UTR

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

Firefly Luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

pTET2

Available since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe EGFR(L861Q)

Plasmid

#32068

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

L861Q

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
CD55-bio-His

Plasmid

#53152

Purpose

Expresses full-length CD55 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

CD55 (CD55 Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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TPC2-mCherry

Plasmid

#135183

Purpose

Expresses TPC2 with C-terminus mCherry tag in mammalian cells

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TPC2 (Tpcn2 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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