-
Plasmid#203830PurposeLuciferase reporter plasmid for Igf1 enhancer 1DepositorInsertE1
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT353
Plasmid#204506PurposeIn vitro transcription of RNA probes for in situ hybridization of CeRep55 lncRNAs in nematode (after linearization with NotI and BglI)DepositorInsertCeRep55 tandem repeats from Y73B3A (C. elegans)
UseOtherTagsExpressionMutationPromoterdouble-T7Available sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT203
Plasmid#204512PurposeBacterial expression of C04F12.1 with 6xHis and maltose binding protein tags for in vitro assaysDepositorInsertC04F12.1 (C. elegans)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT239
Plasmid#204522PurposeIn vitro transcription of Mos1 transposase mRNA with SL1 and poly(A) used for microinjection into nematodeDepositorInsertMos1_transposase::glh-2_3'UTR (glh-2 Nematode)
UseOtherTagsExpressionMutationPromoterT7 (minimum seq.)Available sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT517
Plasmid#204518PurposeBacterial expression of CEC-4 with 6xHis and maltose binding protein tags for in vitro assaysDepositorInsertcec-4 (C. elegans)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-Halo-10-PFN1-G118V
Plasmid#204225PurposeExpression of Halo-Profilin-1(G118V) in tissue culture cellsDepositorInsertPFN1 (PFN1 Human)
UseTagsHalo, TEV site, SGLRSRAQAS linkerExpressionMammalianMutationG118VPromoterCMVAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-Halo-10-PFN1-Y6D
Plasmid#204223PurposeExpression of Halo-Profilin-1(Y6D) in tissue culture cellsDepositorInsertPFN1 (PFN1 Human)
UseTagsHalo, TEV site, SGLRSRAQAS linkerExpressionMammalianMutationY6DPromoterCMVAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-Halo-10-PFN1-R88E
Plasmid#204224PurposeExpression of Halo-Profilin-1(R88E) in tissue culture cellsDepositorInsertPFN1 (PFN1 Human)
UseTagsHalo, TEV site, SGLRSRAQAS linkerExpressionMammalianMutationR88EPromoterCMVAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTHY_P0019_T2c_BCD_0.1K
Plasmid#200057PurposeBicistronic RBS 0.1K PartDepositorInsertBCD 0.1K
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTHY_P0028_T2c_BCD_18K
Plasmid#200048PurposeBicistronic RBS 18K PartDepositorInsertBCD 18K
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTHY_P0021_T2c_BCD_0.45K
Plasmid#200055PurposeBicistronic RBS 0.45K PartDepositorInsertBCD 0.45K
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTHY_P0020_T2c_BCD_0.2K
Plasmid#200056PurposeBicistronic RBS 0.2K PartDepositorInsertBCD 0.2K
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTHY_P0026_T2c_BCD_8.5K
Plasmid#200050PurposeBicistronic RBS 8.5K PartDepositorInsertBCD 8.5K
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.11_Nued2_E4
Plasmid#203833PurposeLuciferase reporter plasmid for Igf1 enhancer 4DepositorInsertE4
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.11_Nued2_E2
Plasmid#203831PurposeLuciferase reporter plasmid for Igf1 enhancer 2DepositorInsertE2
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.11_Nued2_E3
Plasmid#203832PurposeLuciferase reporter plasmid for Igf1 enhancer 3DepositorInsertE3
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-pnRFP
Plasmid#201678PurposeBacteria protein expression of pnRFP biosensorDepositorInsertpnRFP
UseTags6xHis tagExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcBAC3-POLY-pnRFP
Plasmid#201680PurposeBoost the expression of pnRFP in mammalian cells (e.g., macrophage RAW 264.7 cells)DepositorInsert813
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMAH-pnRFP-B30Y
Plasmid#201823PurposeExpresses pnRFP-B30Y in mammalian cellsDepositorInsertpnRFP-B30Y
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-NLS-ZapCV2-ALA
Plasmid#203665PurposeExpresses zinc dead sensor ZapCV2-Ala in the nucleus of mammalian cellsDepositorInsertZapCV2-Ala zinc dead sensor
UseTagsSV40 NLSExpressionMammalianMutationCys and His in Zap1 is mutated with AlaPromoterCMV promoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only