We narrowed to 7,394 results for: 11

1. pET28a_PA4114_Cterm_His

   Plasmid

   #220484

   Purpose

   heterologous expression of PA4114 for protein purification

   Depositor

   Emily Balskus

   Article

   Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

   Insert

   PA4114
   

   Tags

   6xHs

   Expression

   Bacterial

   Mutation

   none

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Orai1

   Plasmid

   #12199

   Depositor

   Anjana Rao

   Article

   Feske et al Nature. 2006 May 11. 441(7090):179-85.

   Insert

   Orai1 (ORAI1 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   July 20, 2006

   Availability

   Academic Institutions and Nonprofits only

3. TN-XXL pcDNA3

   Plasmid

   #45797

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Oliver Griesbeck

   Article

   Mank et al Nat Methods. 2008 Sep;5(9):805-11. doi:

   Insert

   TN-XXL
   

   Tags

   ECFP and YFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pJB332

   Plasmid

   #115949

   Purpose

   ORF insert for replicon MoClo

   Depositor

   Ron Weiss

   Article

   Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

   Insert

   Lvl0 TetR-Gly9-DDX6 (contains SapI)
   

   Use

   Synthetic Biology

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTW122

   Plasmid

   #115962

   Purpose

   ORF insert for replicon MoClo

   Depositor

   Ron Weiss

   Article

   Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

   Insert

   Lvl0 5'-2xTetApt-mVenus-PEST
   

   Use

   Synthetic Biology

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV5 Flag-Smad1 (AAVA)

   Plasmid

   #14957

   Depositor

   Joan Massague

   Article

   Kretzschmar et al Genes Dev. 1997 Apr 15. 11(8):984-95.

   Insert

   Smad1 (AAVA) (SMAD1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Three Serine to Alanine mutations in the C-termin…

   Available Since

   May 17, 2007

   Availability

   Academic Institutions and Nonprofits only

7. Cx43K258stop-msfGFP

   Plasmid

   #69023

   Purpose

   Expresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with msfGFP on the C-terminus. CMV promoter.

   Depositor

   David Spray

   Article

   Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

   Insert

   Cx43 (Gja1 Rat)
   

   Tags

   V206K monomerized super folder GFP

   Expression

   Mammalian

   Mutation

   delete codons 258-381 and fuse in frame to monome…

   Promoter

   CMV

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. MTK8b_004

   Plasmid

   #123892

   Purpose

   Encodes the 3rd gen. 5' HIV LTR cassette as a type 8b part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   5' HIV LTR (3rd gen.)
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-CreLite

   Plasmid

   #131782

   Purpose

   Middle entry vector (Tol2 kit) with bi-cistronic CreLite system components, PhyBΔCreC and PIF6CreN, linked by P2A.

   Depositor

   George Eisenhoffer

   Article

   Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

   Insert

   PhyBCreC-P2A-PIF6CreN
   

   Use

   Cre/Lox; Gateway cloning

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMIY-mETV3-Flag

    Plasmid

    #34579

    Depositor

    Peter Murray

    Article

    El Kasmi et al J Immunol. 2007 Dec 1;179(11):7215-9.

    Insert

    Etv3 (Etv3 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG and IRES-YFP

    Expression

    Mammalian

    Available Since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pCAG-GFP-PQRv3-RFP

    Plasmid

    #73951

    Purpose

    Protein Quantitation Reporter (PQR) to quantify a protein of interest in mammalian cells.

    Depositor

    Brian Chen

    Article

    Lo et al Cell Rep. 2015 Dec 22;13(11):2634-44. do

    Inserts

    sfGFP (superfolder GFP)
    RFP
    

    Tags

    A residual PQR peptide wii be left at the C-termi…

    Expression

    Mammalian

    Promoter

    Chicken beta actin and Chicken beta actin (shared…

    Available Since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

12. GW1-mito-mRuby2-roGFP1

    Plasmid

    #107219

    Purpose

    Mammalian expression plasmid to target the rmRuby1-roGFP1 sensor to the mitochondrial matrix.

    Depositor

    Mathew Tantama

    Article

    Norcross et al ACS Sens. 2017 Nov 22;2(11):1721-1729. d

    Insert

    mRuby2-roGFP1
    

    Tags

    4xCox8

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIRESneo2 mycBioID-Tollip

    Plasmid

    #178054

    Purpose

    myc-BioID fused to human Tollip cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.

    Depositor

    David Tumbarello

    Article

    Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

    Insert

    Toll-Interacting Protein (TOLLIP Human)
    

    Tags

    mycBioID

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. MTK7_014

    Plasmid

    #123881

    Purpose

    Encodes the extended 3rd gen. 3' HIV LTR cassette as a type 7 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    3' HIV LTR (3
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. MTK3b_031

    Plasmid

    #123809

    Purpose

    Encodes Kruppel Associated Box repression domain fused to methyl CpG binding protein 2 as a Type 3b part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    KRAB:meCP2
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. HOXA7 (murine) His-tag pET

    Plasmid

    #8491

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HOXA7 (Hoxa7 Mouse)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Fusion sequence is: ggatccATG; makes protein by …

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

17. GEV 1

    Plasmid

    #12615

    Depositor

    John Louis

    Article

    Huth et al Protein Sci. 1997 Nov . 6(11):2359-64.

    Insert

    GB1 domain of protein G as N terminal tag, with Bcl2 as insert (Bcl2 Mouse)
    

    Tags

    G protein and His

    Expression

    Bacterial

    Available Since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR_PGK_antiCD19_Z3_Gal4VP64

    Plasmid

    #169916

    Purpose

    Expression of a synNotch receptor containing antiCD19, a zebrafish Notch core and Gal4VP64.

    Depositor

    Zhili Rong

    Article

    Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

    Insert

    antiCD19-Notch3-GL4VP64
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-mCherry-LKB1(KD)

    Plasmid

    #84640

    Purpose

    mCherry-tagged, LKB1(K78I); kinase-dead mutant

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    mCherry-LBK1(K78I) (STK11 Human, Synthetic)
    

    Tags

    6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

    Expression

    Mammalian

    Mutation

    Lysine 78 mutated to Isoleucine; kinase dead

    Promoter

    CMV

    Available Since

    May 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pB-Neo-rTOM20-GFP

    Plasmid

    #210331

    Purpose

    Overexpression of rTOM20

    Depositor

    Li Yu

    Article

    Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

    Insert

    rTOM20 (Tomm20 Rat)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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