We narrowed to 7,349 results for: 11

1. JPF0512

   Plasmid

   #124003

   Purpose

   Encodes promoter-less expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   PB_spacer-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. MTK3_027

   Plasmid

   #123742

   Purpose

   Encodes bicistronic ABI gene expression system as a Type 3 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   VP16::PYLcs-T2A-GAL4DBD::ABIcs
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti6.3/V5-DEST-PKCi-A120E-ΔPB1

   Plasmid

   #56658

   Purpose

   expresses constitutively active human PKCi with a deletion on the PB1 domain

   Depositor

   Pedro Salas

   Article

   Forteza et al Biol Open. 2013 Oct 8;2(11):1264-9. doi:

   Insert

   Human PKCi (PRKCI Human)
   

   Use

   Lentiviral

   Tags

   V5/His

   Mutation

   A120E and deletion of PB1 domain (aa 24–98)

   Promoter

   CMV

   Available Since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-HA-REDD1 R133A/P139A (#626)

   Plasmid

   #99946

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

   Insert

   REDD1 (DDIT4 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   R133A/P139A

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. MTK0_002

   Plasmid

   #123960

   Purpose

   Encodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator as a type 0 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   PB Destination - 2xHS4 - HygroR
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTK5_008

   Plasmid

   #123852

   Purpose

   Encodes ConRE' and the chicken hypersensitivity site 4 insulator as a Type 5 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Connector RE'-2xHS4
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEasyG2_zeo

   Plasmid

   #184913

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_zeo recombines in vivo with a PCR product from pEasyG2_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEasyG2_nat

   Plasmid

   #184914

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_nat recombines in vivo with a PCR product from pEasyG2_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEasyG3_zeo

   Plasmid

   #184917

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_zeo recombines in vivo with a PCR product from pEasyG3_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. ARB342

    Plasmid

    #124025

    Purpose

    Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear mAzamiGreen

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    LS-A4_EF1a-NLS::mAzamiGreen-bgPA_R1
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MTK1_027

    Plasmid

    #123689

    Purpose

    Encodes ConL5 and human E4 insulator as a Type 1 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Connector L5-E4 insulator
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MTK1_028

    Plasmid

    #123690

    Purpose

    Encodes ConL6 and human F1 insulator as a Type 1 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Connector L6-F1 insulator
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MTK1_029

    Plasmid

    #123691

    Purpose

    Encodes ConL7 and human B2 insulator as a Type 1 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Connector L7-B2 insulator
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. JPF0511

    Plasmid

    #124002

    Purpose

    Encodes pHUBC driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_phubc-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. JPF0520

    Plasmid

    #124011

    Purpose

    Encodes pCMV driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pCMV-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT2Apuro-TRE-synPCB2.0

    Plasmid

    #139481

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

    Insert

    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
    

    Use

    Tol2 transposon donor vector

    Expression

    Mammalian

    Promoter

    Tet responsive element

    Available Since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLpC-Zipp.-L-IFP-F[1]+Plum (mammalian)

    Plasmid

    #52907

    Purpose

    Binary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[1]. In the second MCS, the Plum fluorescent protein is cloned in between PmeI/SalI. Confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    Zipp.-L-IFP-F[1]+Plum
    

    Use

    Retroviral

    Tags

    L-IFP-F[1]

    Expression

    Mammalian

    Promoter

    Unknown

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pSuper.Retro.Puro-WDR5 shRNA 4

    Plasmid

    #59977

    Purpose

    Knockdown of WDR5

    Depositor

    Debu Chakravarti

    Article

    Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

    Insert

    WDR5 shRNA
    

    Use

    Retroviral

    Available Since

    Oct. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pRLCon1-3180

    Plasmid

    #31445

    Depositor

    Gerhart Ryffel

    Article

    Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

    Insert

    HNF4A 3'UTR (long) (HNF4A Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCfB3046(gRNA XI-5)

    Plasmid

    #73288

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-5

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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