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pMSC264

Plasmid

#216363

Purpose

xhNup98-Nb2i Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup98-Nb2i

Use

Affinity Reagent/ Antibody

Tags

14xHis-Avi-SUMOEu1

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pDG04477

Plasmid

#216352

Purpose

xhNup35-Nb1t Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup35-Nb1t

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSCL390

Plasmid

#219500

Purpose

Integrating inducible cassette (Cas9-P2A-Csy4 and Eco1 RT) for yeast overexpression

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

Insert

Integrating inducible cassette: Cas9-P2A-Csy4 and Eco1 RT

Tags

SV40-NLS

Expression

Yeast

Promoter

GAL1-GAL10

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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pSCL391

Plasmid

#219501

Purpose

Express Ade2 and Faa1 editrons from a single Gal7 promoter, separated by a Csy4 recognition sequence

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

Insert

Eco1 editing ncRNAs and Cas9 gRNAs to edit ADE2 and FAA1

Expression

Yeast

Promoter

GAL7

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-A51P/A56P

Plasmid

#216400

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56 to proline mutations

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-A51P/A56P

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Alanine 51 and 56 to Proline, and deleted…

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-A51P/A56P/Q443P

Plasmid

#216402

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q443 to proline mutations

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-A51P/A56P/Q443P

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Alanine 51, 56 and Glutamine 443 to Proli…

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-Y4S/Y8S/Y16S

Plasmid

#216405

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y4/Y8/Y16 to serine mutations

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-Y4S/Y8S/Y16S

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Tyrosine 4, 8 and 16 to Serine, and delet…

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-Y64S/Y66S/Y69S

Plasmid

#216406

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y64/Y66/Y69 to serine mutations

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-Y64S/Y66S/Y69S

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Tyrosine 64, 66 and 69 to Serine, and del…

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-Y101S/Y104S/Y106S

Plasmid

#216407

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y101/Y104/Y106 to serine mutations

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-Y101S/Y104S/Y106S

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Tyrosine 101, 104 and 106 to Serine, and …

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-Y401S/Y404S/Y409S

Plasmid

#216409

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y401/Y404/Y409 to serine mutations

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-Y401S/Y404S/Y409S

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Tyrosine 401, 404 and 409 to Serine, and …

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-Y450S/Y452S/Y456S

Plasmid

#216410

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y450/Y452/Y456 to serine mutations

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-Y450S/Y452S/Y456S

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Tyrosine 450, 452 and 456 to Serine, and …

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-Y478S/Y482S/Y484S

Plasmid

#216411

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y478/Y482/Y484 to serine mutations

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-Y478S/Y482S/Y484S

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Tyrosine 478, 482 and 484 to Serine, and …

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-scrCdh10.1 GFP

Plasmid

#209102

Purpose

GFP expressing scramble of shRNA targeting Cdh10

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCdh10.1

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shCdh11.1 GFP

Plasmid

#209103

Purpose

GFP expressing shRNA targeting Cdh11

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

shCdh11.1

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-scrCdh11.1 GFP

Plasmid

#209104

Purpose

GFP expressing scramble of shRNA targeting Cdh11

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCdh11.1

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-scrCtnnd2.2-GFP

Plasmid

#209098

Purpose

GFP expressing scramble of shRNA targeting Ctnnd2 3' UTR

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCtnnd2.2

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-11_humanized-mutant

Plasmid

#214315

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (Casp4 Mouse)

Tags

His-TEV

Expression

Bacterial

Mutation

N152K_KLS288YKT_KASIHS348TPRAKA

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCF823_EFS-Cas9-P2A-Puro

Plasmid

#211645

Purpose

EFS-Cas9-P2A-Puro

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCF226_EFS-Cas9-P2A-Puro

Plasmid

#211644

Purpose

EFS-Cas9-P2A-Puro

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-scrCtnnd2.1-mCherry-CAAX

Plasmid

#209108

Purpose

Membrane tethered mCherry scramble of shRNA targeting Ctnnd2 N-terminus

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCtnnd2.1

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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