Addgene

We narrowed to 7,488 results for: puc

Showing: 3301 - 3320 of 7488 results

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pKSJ331

Plasmid

#103063

Purpose

Expresses colicin E1 and E1 immunity protein without E1 lysis (kil) gene

Depositor

Alan Finkelstein, Karen Jakes

Article

Kienker et al J Gen Physiol. 2008 Dec;132(6):693-707.

Insert

Colicin E1 and E1 immunity protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

colicin promotor from ColE1

Available since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pAC37

Plasmid

#111618

Purpose

Fluorescent biosensor for gauging the activity of the PQS quorum sensing system in Pseudomonas aeruginosa

Depositor

Tim Tolker-Nielsen

Article

Yang et al Microbiology. 2007 May;153(Pt 5):1318-28

Insert

PpqsA-gfp(ASV)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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FUS_Halo_N_allele_400

Plasmid

#161767

Purpose

Donor vector for endogenous tagging of human FUS at the N-terminus with halotag

Depositor

Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

Insert

halotag (FUS Human)

Use

Donor vector for endogenous tagging

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAX1

Plasmid

#117397

Purpose

allelic exchange vector with GFP merodiploid tracker and temperature-sensitive origin of replication

Depositor

Karen Guillemin

Article

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pESR1-donor

Plasmid

#112337

Purpose

CRISPR donor plasmid to tag human transcription factor ESR1 with GFP

Depositor

Kevin White

Insert

ESR1 homology arms flanking EGFP-IRES-Neo cassette (ESR1 Human)

Use

CRISPR

Tags

Expression

Mutation

Unknown

Promoter

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pH1v1

Plasmid

#60244

Purpose

gRNA expression by the H1 promoter

Depositor

Donald Zack

Article

Ranganathan et al Nat Commun. 2014 Aug 8;5:4516. doi: 10.1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

H1 promoter

Available since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pmCherry-GFP

Plasmid

#86441

Purpose

visualization and quantifying the out-of-frame mutations from genome editing

Depositor

Yun-Bo Shi

Article

Fu et al Sci Rep. 2016 Oct 17;6:35488. doi: 10.10

Insert

mCherry-MCS-sfGFP fusion gene

Use

Color visualization in bacteria

Tags

mCherry-sfGFP

Expression

Mutation

Promoter

Plac

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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FUS_Halo_C_allele_400

Plasmid

#161768

Purpose

Donor vector for endogenous tagging of human FUS at the C-terminus with halotag

Depositor

Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

Insert

halotag (FUS Human)

Use

Donor vector for endogenous tagging

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQD0KN0285

Plasmid

#162290

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGGF001

Plasmid

#48842

Purpose

Provides a BASTA resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pMAS::BastaR::tMAS

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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LI D-E mOrange + link c2

Plasmid

#54421

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

16 aa GS linker + mOrange

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pRP(Exp)-CMV> :dTomato:IRES:Puro

Plasmid

#110832

Purpose

Expresses dTomato (dT) red fluorescent reporter.

Depositor

Ande Satyanarayana

Article

Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:

Insert

dTomato Tag

Use

Non-viral gene expression vector

Tags

IRES

Expression

Mutation

Promoter

CMV

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pTS1106_Tier3(Lenti)

Plasmid

#169661

Purpose

Tier-3 vector for stable integration of up to three cassettes via lentiviral transduction (PCMV-5'LTR-Psi-gag-env-A1::A2::A3-WPRE-3'LTR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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AP575-1

Plasmid

#71277

Purpose

Expresses 3Xflag::tagRFP::myc in bacteria

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

tagRFP

Use

Tags

3xFlag and myc

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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TC550

Plasmid

#164891

Purpose

Punc-4::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

unc4-rtTA(Q)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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LI E-F HSP-T

Plasmid

#54432

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

Heat Shock Protein terminator A.thaliana

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pERF-donor

Plasmid

#112299

Purpose

CRISPR donor plasmid to tag human transcription factor ERF with GFP

Depositor

Kevin White

Insert

ERF homology arms flanking EGFP-IRES-Neo cassette (ERF Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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LI F-G neo c2

Plasmid

#54405

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

neomycin phosphotransferase II (nptII)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LI D-E HA

Plasmid

#54449

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

human influenza hemagglutinin (HA) tag (YPYDVPDYA)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

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LI B-C HA

Plasmid

#54448

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

human influenza hemagglutinin (HA) tag (YPYDVPDYA)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

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