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Plasmid#27629DepositorInsertmouse myc ULK1 (Myc Mouse)
UseTagsmycExpressionMammalianMutationInitial M from ULK1 removed. Silent mutation at A…PromoterAvailable sinceFeb. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMG058 MBP-GS 634-666-His
Plasmid#154077PurposeExpresses MBP-GS 634-666-His (human 33aa Glycogen Synthase peptide as a fusion protein with MBP and His- tags) in E.coliDepositorInsertGYS1 (GYS1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterTacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-Sox17
Plasmid#128828Purposedox-dependent expression of mouse Sox17DepositorInsertSox17 (Sox17 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-mouseGIRK2-eGFP-StII-6xHis
Plasmid#124277PurposeExpresses mouse GIRK2 as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tagDepositorInsertGIRK2 (Kcnj6 Mouse)
UseTagseGFP-StreptagII-6xHisExpressionYeastMutationPromoterAOX1Available sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ 2xFLAG-2xSTREP_BACH1_Y11F
Plasmid#159132PurposeExpresses BACH1 in mammalian cellsDepositorInsertBACH1 (BACH1 Human)
UseTags2X-FLAG _ 2X-STREPExpressionMammalianMutationY11FPromoterCMVAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5-H1-shRNA-CMV-HA-PACSIN1
Plasmid#72577PurposeBicistronic construct expressing PACSIN1 shRNA and HA-PACSIN1-rescue constructDepositorInsertsUseRNAiTagsHAExpressionMammalianMutation5 silent mutations around shRNA recognition seque…PromoterCMV and H1Available sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJH130
Plasmid#162484PurposeSEC plasmid containing LG1 homology arms and myo-3p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.DepositorInsertmyo-3p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
UseCRISPRTagsmyo-3p::TIR1::F2A::AID*::NLS::tbb-2 3'UTRExpressionWormMutationPromoterAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
OA-1136J
Plasmid#153023PurposeExpress His-MBP-TEV-CasRx recombinant proteinDepositorInsertCasRx
UseTagsHis-MBP-TEVExpressionBacterialMutationPromoterT7Available sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH126
Plasmid#162482PurposeSEC plasmid containing LG1 homology arms and vha-8p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.DepositorInsertvha-8p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
UseCRISPRTagsvha-8p::TIR1::F2A::AID*::NLS::tbb-2 3'UTRExpressionWormMutationPromoterAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-mouseGIRK2R201A-eGFP-StII-6xHis
Plasmid#124278PurposeExpresses mouse GIRK2 R201A as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tagDepositorInsertGIRK2 (Kcnj6 Mouse)
UseTagseGFP-StreptagII-6xHisExpressionYeastMutationR201APromoterAOX1Available sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 - myc ULK1 wt
Plasmid#27623DepositorInsertmouse myc ULK1 (Myc Mouse)
UseGateway donr vectorTagsmycExpressionMutationInitial M from ULK1 removed. Silent mutation at A…PromoterAvailable sinceFeb. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMS04 [myo-3p::bPGC::SL2::mCherry]
Plasmid#168172PurposeExpression of bPGC in BWMs of C. elegansDepositorInsertbPGC
UseTagsExpressionWormMutationPromoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOUPc-UL148-mCherry
Plasmid#179279PurposeAll in one "tet-on" lentivirus vector that expresses human cytomegalovirus UL148 fused to an mCherry tag at its cytoplasmic tail. UL148 reorganizes the ER and activates the UPR.DepositorInsertUL148 fused to mCherry
UseLentiviral; Tet-on, all-in-one lentiviral vectorTagsmCherryExpressionMammalianMutationPromoterTet responsive element 3GAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKTop::tse5-CT (1169-1281)
Plasmid#192955PurposeFor membrane insertion study; Expresses tse5-CT (1169-1281) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminusDepositorInserttse5-CT (1169-1281)
UseTagsExpressionBacterialMutationencodes for tse5 (1169-1281)PromoterAvailable sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-cGAS R71/75E C396/7A-HA
Plasmid#130920PurposeExpresses human cGAS with R71/75E and C396/397A point mutations; Puromycin selection markerDepositorInserthuman cGAS R71/75E C396/7A
UseTagsHAExpressionMammalianMutationaa 71 and 75 mutated to glutamate; aa 396 and 397…PromoterCMVAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 2xFLAG-2xSTREP EMI1
Plasmid#158947PurposeExpress human EMI1 in mammalian cellsDepositorInsertEMI1 (FBXO5 Human)
UseTags2X FLAG 2X STREPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
PX-TIR-GFP
Plasmid#52737PurposeRetroviral vector expresses the lipid binding domain of p40-phox fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable markerDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTagsGFP and p40 Phox lipid binding domainExpressionMammalianMutationDeleted amino acids 1-85PromoterAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 NDUFS6(5’UTR)-FF
Plasmid#85488PurposeFirefly luciferase under the control of NDUFS6 5'UTRDepositorInsert5'UTR of NDUFS6 (NDUFS6 Human)
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCR8-CHD5-FRAGMENT
Plasmid#68879PurposeGW entry vector for CHD5DepositorInsertCHD5 (CHD5 Human)
UseGateway entry vectorTagsExpressionMutation331-672aaPromoternoneAvailable sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G_NT-GSDMD_I105N_NoCys
Plasmid#201177PurposeDox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transductionDepositorInsertGasdermin D N-terminal domain (Gsdmd Mouse)
UseRetroviralTagsExpressionMutationaa1-276 only, I105N, C39A, C57A, C77A, C122A, C2…PromoterTRE3GAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only