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MT2a_K31A

Plasmid

#105695

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a K31A mutant

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Use

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Lysine 31 to Alanine

Promoter

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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MT2a_K30A K31A

Plasmid

#105696

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a K30/31A mutant

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Use

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Lysine 30 and 31 to Alanine

Promoter

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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MT2a_C21A

Plasmid

#105697

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a C21A mutant

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Use

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Cysteine 21 to Alanine

Promoter

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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MT2a_K20A

Plasmid

#105698

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a K20A mutant

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Use

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Lysine 20 to Alanine

Promoter

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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MT2a_S6A

Plasmid

#105699

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a S6A mutant

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Use

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Serine 6 to Alanine

Promoter

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET20b-YbAnbu

Plasmid

#129637

Purpose

Expression plasmid of Anbu from Y. bercovieri (UNIPROT A0A0T9RXH3 variant with V14A and G107H mutations)

Depositor

Matthias Bochtler

Article

Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

Insert

YbAnbu

Use

Tags

His6-tag

Expression

Bacterial

Mutation

V14A and G107H

Promoter

T7

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET20b-YbAnbuT1A

Plasmid

#129638

Purpose

Expression plasmid of Anbu from Y. bercovieri (inactive mutant; (UNIPROT A0A0T9RXH3 variant with T1A, V14A and G107H mutations)

Depositor

Matthias Bochtler

Article

Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

Insert

YbAnbuT1A

Use

Tags

His6-tag

Expression

Bacterial

Mutation

T1A, V14A and G107H

Promoter

T7

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_EGFP-LAMA-S98 (for p24)

Plasmid

#130717

Purpose

Plasmid encoding the bacterial expression of LAMA-S98 for p24 HIV Capsid protein

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

EGFP-LAMA-S98

Use

Tags

Hisx10 and StrepTag

Expression

Bacterial

Mutation

Deleted first Met, Ala and Last Ser from the Enha…

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJL1-neoR

Plasmid

#117048

Purpose

In vitro expression of neoR (kanamycin resistance gene) from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

Insert

neoR

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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rs210-major

Plasmid

#113377

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs2104047 major allele (A)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs2104047 major allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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rs210-minor

Plasmid

#113378

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs2104047 minor allele (G)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs2104047 minor allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
rs438-major

Plasmid

#113369

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs4384382 major allele (A)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs4384382 major allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only
rs344-minor

Plasmid

#113366

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs34466261 minor allele (A)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs34466261 minor allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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rs438-minor

Plasmid

#113370

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs4384382 minor allele (G)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs4384382 minor allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
rs294-minor

Plasmid

#113374

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs2941742 minor allele (G)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs2941742 minor allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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rs294-major

Plasmid

#113373

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs2941742 major allele (A)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs2941742 major allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
rs281-minor

Plasmid

#113372

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs281038 minor allele (G)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs281038 minor allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
rs281-major

Plasmid

#113371

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs281038 major allele (A)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs281038 major allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
rs117-major

Plasmid

#113367

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs11716984 major allele (A)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs11716984 major allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
rs117-minor

Plasmid

#113368

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs11716984 minor allele (T)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs11716984 minor allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge