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RNAPII_CTD38-52
Plasmid#98685PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase IIDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationheptads 38-52, residues 1853-1970PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
C239-E03: attB2r-IRES-ffLuc2-pA-attB3
Plasmid#162919PurposeGateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2 reporter using an internal ribosome entry sequence (IRES)DepositorInsertfirefly luciferase with upstream IRES sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
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RNAPII_CTD27-37
Plasmid#98686PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase IIDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationheptads 27-37, residues 1773-1852PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBT100-neo
Plasmid#123137PurposeGolden Gate donor vector for the assembly of EGFP-based mammalian recombinase reporters.DepositorInsertNeomycin, poly-A terminator
UseSynthetic Biology; Golden gate donor vectorTagsExpressionMutationPromoternoneAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
p224 pCMV-RFP/CREM
Plasmid#8401DepositorInsertRFP/CREM
UseCre/LoxTagsExpressionMammalianMutationmodified Cre, LOX 511 sites surrounding RFP withi…PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pPROEX Hsp104 (773-908)
Plasmid#1316DepositorInsertHsp104 (773-908) (HSP104 Budding Yeast)
UseTags6X His + TEV cleavageExpressionBacterialMutationdeletion of aa1-773PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pPROEX Hsp104 (361-548)
Plasmid#1317DepositorInsertHsp104 (361-548) (HSP104 Budding Yeast)
UseTags6X His + TEV cleavageExpressionBacterialMutationaa361-548 of Hsp104PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pFS217-USP44_MSCV-TAP
Plasmid#43896PurposeRetroviral expression of USP44 in mammalian cellsDepositorInsertUSP44 (USP44 Human)
UseRetroviralTagsFLAG, HA, and HISExpressionMammalianMutationPromoterAvailable sinceMay 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSP303 pRS306 pRPS2-Far1DS-NLS-mCherry
Plasmid#68279PurposeSKARS: Fus3 MAPK activity sensor: Far1DS 2xNLS mCherryDepositorInsertFar1DS NLS mCherry
UseTagsmCherryExpressionYeastMutationPromoterRPS2Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUAS-hopscotch
Plasmid#37301DepositorInserthopscotch (hop Fly)
UseTagsExpressionInsectMutationPromoterHsp70Available sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pED73 pRS304 pRPS2-SteDS-NLS-mCitrine
Plasmid#68278PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCitrineDepositorInsertSte7DS NLS mCitrine
UseTagsmCitrineExpressionYeastMutationPromoterpRPS2Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAM-PAT-ProGL2 GW
Plasmid#80689Purposebinary DEST vector for plant expressionDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterProGL2Available sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSALECTNK-LGK/csTEM1
Plasmid#81164PurposepSALECT plasmid with levoglucosan kinase fused to TEM-1DepositorInsertLevoglucosan kinase
UseTagsCodon swapped TEM-1 beta-lactamase (H26 to W290) …ExpressionBacterialMutationPromoterlacAvailable sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
ath5:GFP-DCX
Plasmid#105959Purposetransgenesis, doublecortin DCXDepositorInsertDCX (DCX Human)
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD43-52
Plasmid#98684PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase IIDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationhepatds 43-52, residues 1888-1970PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pED45: pRS306 pRPS2-Ste7DS-NLS-mCherry
Plasmid#68274PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCherryDepositorInsertSte7DS NLS mCherry
UseTagsmCherryExpressionYeastMutationPromoterpRPS2Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
p252 pPr-CREM/CMV-STOP-luc
Plasmid#8406DepositorInsertCREM/lox/luciferase
UseCre/LoxTagsExpressionMammalianMutationmodified CrePromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FRT-FAK-FERM10
Plasmid#59126PurposeFAK fret control sensor without kinase domain with 10 nm linkerDepositorInsertFocal Adhesion Kinase
UseTagsFLAG, mCerulean, and mCitrineExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only
