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Showing: 3281 - 3300 of 4772 results

  1. pSHDY*in_PpetE:BCD2:AgBIS:Flag

    Plasmid
    #133971
    Purpose
    Heterologous, copper-inducible expression of (E)-α-bisabolene synthase from Abies grandis (AgBIS) in Synechocystis sp. PCC 6803. The AgBIS CDS is fused to a C-termial Flag-tag.
    Depositor
    Pia Lindberg
    Article
    Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.
    Insert
    AgBIS:Flag
    Use
    Synthetic Biology
    Tags
    Flag
    Expression
    Bacterial
    Mutation
    inserted gene is codon optimized for Synechocyst…
    Promoter
    PpetE:BCD2
    Available since
    April 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133971-plasmid-map-sequence-id-271350

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  2. pSHDY*in_PpetE:BCD2:MrBBS:Strep

    Plasmid
    #133973
    Purpose
    Heterologous, copper-inducible expression of (-)-α- bisabolol synthase from Matricaria recutita (MrBBS) in Synechocystis sp. PCC 6803. The MrBBS CDS is fused to a C-termial StrepII-tag.
    Depositor
    Pia Lindberg
    Article
    Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.
    Insert
    MrBBS:StrepII
    Use
    Synthetic Biology
    Tags
    StrepII
    Expression
    Bacterial
    Mutation
    inserted gene is codon optimized for Synechocyst…
    Promoter
    PpetE:BCD2
    Available since
    April 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133973-plasmid-map-sequence-id-267788

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  5. pX330-Flag-evoSpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126758
    Purpose
    Expression plasmid for human codon-optimized increased fidelity evoSpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    evoSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    M495V, Y515N, K526E, R661Q
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126758-plasmid-map-sequence-id-412365

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  6. pX330-Flag-HeFSpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126759
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    HeFSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126759-plasmid-map-sequence-id-412375

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  8. pX330-Flag-eSpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126754
    Purpose
    Expression plasmid for human codon-optimized increased fidelity eSpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    eSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126754-plasmid-map-sequence-id-410972

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  9. pX330-Flag-SpCas9-HF1 (without sgRNA; with silent mutations)

    Plasmid
    #126755
    Purpose
    Expression plasmid for human codon-optimized increased fidelity SpCas9-HF1 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    SpCas9-HF1
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126755-plasmid-map-sequence-id-410971

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  10. pX330-Flag-HypaSpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126756
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HypaSpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    HypaSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N692A, M694A, Q695A, H698A
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126756-plasmid-map-sequence-id-410579

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  11. PV259

    Plasmid
    #132340
    Purpose
    CasD plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays
    Depositor
    Joshua Young
    Article
    Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.
    Insert
    CasD-AT-CBF1 (CBF1 CasD (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana), Mustard Weed)
    Use
    Tags
    AT-CBF1
    Expression
    Plant
    Mutation
    Codon optimize for expression and stability in Ze…
    Promoter
    Available since
    Oct. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    132340-plasmid-map-sequence-id-258453

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  12. PV503

    Plasmid
    #132342
    Purpose
    CasE plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays
    Depositor
    Joshua Young
    Article
    Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.
    Insert
    CasE-AT-CBF1 (CBF1 CasE (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana), Mustard Weed)
    Use
    Tags
    AT-CBF1
    Expression
    Plant
    Mutation
    Codon optimize for expression and stability in Ze…
    Promoter
    Available since
    Oct. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    132342-plasmid-map-sequence-id-258473

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  13. PV504

    Plasmid
    #132343
    Purpose
    CasA plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays
    Depositor
    Joshua Young
    Article
    Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.
    Insert
    CasA-AT-CBF1 (CBF1 Mustard Weed, CasA (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana))
    Use
    Tags
    AT-CBF1
    Expression
    Plant
    Mutation
    Codon optimize for expression and stability in Ze…
    Promoter
    Available since
    Oct. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    132343-plasmid-map-sequence-id-258480

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  14. pCEP4-191084-QES.i06.c07

    Plasmid
    #123239
    Purpose
    Mammalian expression plasmid for Env from the 191084 B7-19 HIV-1 isolate; QES mutant with enhanced binding to PG16
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (191084 B7-19) Env
    Use
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Mutations P124D, …
    Promoter
    CMV
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    123239-plasmid-map-sequence-id-235191

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  15. pGEX-4T-3-cytXlATL2

    Plasmid
    #124063
    Purpose
    Fragment of Xenopus laevis Atlastin (cytATL). This fragment contains the GTPase domain and the three-helix bundle, but lacks the transmembrane segments and the cytosolic tail.
    Depositor
    Tom Rapoport
    Article
    Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d
    Insert
    cytXIATL2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    May 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    124063-plasmid-map-sequence-id-243791

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  16. pCEP4-Q842-QES.i04.c05

    Plasmid
    #123233
    Purpose
    Mammalian expression plasmid for Env from the Q842.d12 HIV-1 isolate; QES mutant with enhanced binding to PG16
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (Q842.d12) Env
    Use
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Mutations P124D, …
    Promoter
    CMV
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    123233-plasmid-map-sequence-id-235181

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  17. pCEP4-Q769-QES.i03.c04

    Plasmid
    #123229
    Purpose
    Mammalian expression plasmid for Env from the Q769.d22 HIV-1 isolate; QES mutant with enhanced binding to PG16
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (Q769.d22) Env
    Use
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Mutations A200E, …
    Promoter
    CMV
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    123229-plasmid-map-sequence-id-235175

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  18. pCEP4-H031-QES.i07.c09

    Plasmid
    #123271
    Purpose
    Mammalian expression plasmid for Env from the H031 HIV-1 isolate; QES mutant for enhanced presentation of quaternary epitopes
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (H031) Env
    Use
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Mutations S49D, F…
    Promoter
    CMV
    Available since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123271-plasmid-map-sequence-id-235234

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  19. pCEP4-SHIV327C-HuA10-QES.i09.c07

    Plasmid
    #123269
    Purpose
    Mammalian expression plasmid for Env from the SHIV327C-Hu A10 strain; QES mutant for enhanced presentation of quaternary epitopes
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    SHIV327C-Hu A10 Env
    Use
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Mutations V208M, …
    Promoter
    CMV
    Available since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123269-plasmid-map-sequence-id-235232

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  20. pCEP4-Q842gp160-QES.04

    Plasmid
    #100933
    Purpose
    Mammalian expression plasmid for Env from the Q842.d12 HIV-1 isolate; QES mutant with enhanced binding to antibody PG16
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (Q842.d12) Env
    Use
    Tags
    CD5 leader sequence
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; mutations P124D;F…
    Promoter
    CMV
    Available since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    100933-plasmid-map-sequence-id-197641

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Showing: 3281 - 3300 of 4772 results