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Plasmid#13605DepositorInsertintegrin alpha 9 (ITGA9 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPuro3.1(+)_Strep-Lrrk2(delGTPase)-Myc
Plasmid#161587Purposeexpression of Myc-tagged mouse Lrrk2 without GTPase domainDepositorInsertLrrk2 (Lrrk2 Mouse)
UseTagsMyc tag and StrepIIExpressionMammalianMutationdeletion: AA1023-1879PromoterCMVAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPuro3.1(+)_Strep-Lrrk2(delKinase)-Myc
Plasmid#161586Purposeexpression of Myc-tagged mouse Lrrk2 without kinase domainDepositorInsertLrrk2 (Lrrk2 Mouse)
UseTagsMyc tag and StrepIIExpressionMammalianMutationdeletion: AA1871-2540PromoterCMVAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
p7270 MSCV-N-HA-IRES-PURO Empty
Plasmid#163302PurposeEmpty vector controlDepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIVTR(T7A)-CRE-T2A-PuroR
Plasmid#172298PurposeIn vitro transcription of CRE-T2A-PuroR synthetic construct for the excision of floxed cassettesDepositorInsertCRE-T2A-PuroR
UseCre/LoxTagsExpressionMutationPromoterT7 promoter A-initiatingAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA-PGK-puromycin-AflII
Plasmid#106404PurposeEmpty guide RNA cloning vector with an AflII site added from Addgene 41824DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterU6Available sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe-TetCMV-puro-mCerulean-PA-Rac1
Plasmid#22031DepositorInsertmCerulean-PA-Rac1 (RAC1 Human, Avena sativa (oat))
UseRetroviralTagsExpressionMammalianMutationRac1 starts at I4 and contains mutations Q61L, E9…PromoterAvailable sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
Direct-seq_lentiGuide-Puro-Tail-8A8G
Plasmid#157981PurposeExpresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.DepositorInsertS. pyogenes sgRNA cassette (Tail-8A8G)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-Myr-Flag-IKBKE
Plasmid#15293DepositorInsertIKBKE (IKBKE Human)
UseRetroviralTagsMyr-FlagExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
Ki67 (shuffled Nterm)-mNeonGreen-IRESpuro2
Plasmid#183744PurposeExpresses Ki67 (shuffled Nterm)-mNeonGreenDepositorInsertMKI67 (shuffled Nterm) (MKI67 Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationCD domain with shuffled 4 fragments fused to the …PromoterCMVAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2ABFP-W
Plasmid#163178PurposeLentiviral gRNA plasmid targeting human YAP1 gene, co-expression of BFP tagDepositorInsertYAP1 (YAP1 Human)
UseCRISPR and LentiviralTagsBFPExpressionMammalianMutationPromoterU6Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro/Flag PD-L1 N200Q/N219Q
Plasmid#121454Purposeexpression of human PD-L1 mutant in mammalian cellsDepositorInsertCD274 (CD274 Human)
UseLentiviralTagsFlagExpressionMammalianMutationN200Q/N219QPromoterCMVAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
1120 pBabe puroL HA FKHR H215R
Plasmid#9026DepositorInsertFKHR H215R (FOXO1 Human)
UseRetroviralTagsHAExpressionMammalianMutationH215RPromoterAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLEX-MAPKAP1Del-FHH-IRES-Puro
Plasmid#120706PurposeExpresses MAPKAP1 Truncation-Flag-HA-6xHIS fusion protein in mammalian cells & for virus productionDepositorInsertMAPKAP1 Isoform 1
UseLentiviralTagsFlag-HA-6xHISExpressionMammalianMutationDeleted amino acids 193-522PromoterCMVAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-MCS2-EF1-Puro-RGS7
Plasmid#113728PurposeExpress RGS7 in mamalian cellsDepositorInsertRGS7 (RGS7 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-mNG-eDHFR(69K6)-p85iSH2
Plasmid#172103PurposeMammalian expression of p85iSH2 fused to mNeonGreen-eDHFR(69K6)DepositorInsertmNeonGreen-eDHFR(69K6)-p85iSH2 (Pik3r2 Mouse)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-puro-hRAF1-shRNA-1
Plasmid#185371PurposeFor tetracycline-inducible mammalian expression of shRNA: CATGAGTATTTAGAGGAAGTA that targets human RAF1DepositorInsertRAF1 (RAF1 Human)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mycBioID-pBabePuro-myc-BirA*HRASG12V
Plasmid#122214PurposeStably expresses myc-BirA* fused to HRASG12V in mammalian cellsDepositorInsertHRASG12V (HRAS Human)
UseRetroviralTagsmyc-BirA*ExpressionMammalianMutationchanged G12 to VPromoterAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
F5A deltaCT S6K1 MSCV puro
Plasmid#8464DepositorInsertf5a delta ctp70 S6K1 (Rps6kb1 Rat)
UseRetroviralTagsmycExpressionMammalianMutationdeletion of 104 c-terminal amino acids mutation …PromoterAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-mNG-eDHFR(69K6)-RasGEF
Plasmid#172104PurposeMammalian expression of RasGRF1(Cdc25) fused to mNeonGreen-eDHFR(69K6)DepositorInsertmNeonGreen-eDHFR(69K6)-RasGRF1(Cdc25) (RASGRF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1-alphaAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only