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Plasmid#167271PurposeOverexpression of the fast variant (E30R) of SNAP-tag in E. coliDepositorInsertSNAPf-tag
UseTagsHis x10ExpressionBacterialMutationE30RPromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPS22 (pET23a-Cas10/Csm/csm2H6N)
Plasmid#70038PurposeBacterial expression of the S. epidermidis Cas10·Csm complexDepositorInsertCas10/Csm complex
UseTags6xHIS on N terminus of Csm2ExpressionBacterialMutationPromoterT7Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-26b-NbVHH05-ALFA-His
Plasmid#171569PurposeBacterial expression of ALFA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-ALFA-His)DepositorInsertNbVHH05-ALFA-His (UBE2J1 Human)
UseTagsALFA-tag/His-tag and PelB signal peptideExpressionBacterialMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-28a+GFP-CAMSAP3 CKK
Plasmid#59033Purposeexpresses CKK domain of CAMSAP3 in E. coliDepositorInsertCAMSAP3 CKK domain (CAMSAP3 Human)
UseTagsHis, Strep, TEV, and sfGFPExpressionBacterialMutationPromoterAvailable sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
HOXA7 (murine) His-tag pET
Plasmid#8491DepositorInsertHOXA7 (Hoxa7 Mouse)
UseTagsHis and T7ExpressionBacterialMutationFusion sequence is: ggatccATG; makes protein by …PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEW3: pET-32a Dot1L(2-416)
Plasmid#124098PurposeDot1L(2-416) expression plasmidDepositorInsertDot1L(2-416) (DOT1L Human)
UseTagsTrx-His-TEVExpressionBacterialMutationPromoterT7Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
AcpP pet29a C-terminal His Tag
Plasmid#75016PurposeExpresses the acyl carrier protein (ACP) from the E. coli fatty acid synthaseDepositorInsertAcpP (acpP E. coli)
UseTagsHis TagExpressionBacterialMutationPromoterT7Available sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
PET-eSpCas9-plus-NLS-6xHis
Plasmid#207380PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInserteSpCas9-plus
UseCRISPRTags6xHisExpressionBacterialMutationK848A, R1060A, amino acids 1005-1013 replaced wit…PromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_His-CBD-SpEndoH
Plasmid#218196PurposeBacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoHDepositorInsertEndoH
UseTagstufA-His-CBDExpressionBacterialMutationcodon optimized Ecoli, residues 45-313PromoterT7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP TEV HDAC8_374
Plasmid#122174PurposeExpresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tagDepositorInsertHis6 MBP TEV HDAC8_374 (HDAC8 Human)
UseTagshexahistidine tag and tobacco etch virus protease…ExpressionBacterialMutationResidues Met1–Pro7 and H375-V377 have been delete…PromoterT7Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-41-4GS-LaG-2
Plasmid#172766PurposeBacterial expression of dimerized anti-GFP nanobodies LaG-41 and LaG-2 (4GS linker)DepositorInsertLaG-41-4GS-LaG-2
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
[pSK122] pETDuet GST-p18(delta5)
Plasmid#136144Purposebacterial expression of GST-p18DepositorInsertGST-p18
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET co-transformation cloning vector (13S-A)
Plasmid#48323DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-29b-3xGluGlu-c-jun-His6
Plasmid#129227PurposeEncodes 3xGluGlu tagged human c-Jun for purification by nickel affinityDepositorInsertcJun (JUN Human)
UseTags6xHistidineExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-Sumo-MBP-CfaN
Plasmid#126016PurposeBacterial expression of MBP-CfaN fusion protein (with N-terminal His5-Sumo)DepositorInsertHis5-Sumo-MBP-CfaN
UseTags5xHis-SUMOExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEW4: pET-32a Dot1L(2-386)
Plasmid#124099PurposeDot1L(2-386) expression plasmidDepositorInsertDot1L(2-386) (DOT1L Human)
UseTagsTrx-His-TEVExpressionBacterialMutationPromoterT7Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28-His8-aCat(660-906)
Plasmid#203333PurposeBacterial expression of alpha-catenin F-actin binding domainDepositorInsertCTNNA1 (CTNNA1 Human)
UseTags8xHis with precision protease cleavage siteExpressionBacterialMutationaa 660-906 onlyPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-his-SUMO-RNF168ring (1-189)
Plasmid#63570PurposeExpression of human RNF168 ring domain (residues 1-189) tagged with His-SUMODepositorInsertRNF168 (RNF168 Human)
UseTags6xHis and SUMO3ExpressionBacterialMutationRing domain only (residues 1-189)PromoterT7Available sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet1-6xHis-TEV-LC3BGlydelta5C
Plasmid#190237PurposePlasmid for the expression and purification of 6xHis-TEV-LC3BGlydelta5C. Internal reference: SMC893.DepositorInsertMAP1LC3B (MAP1LC3B Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET empty polycistronic destination vector (2D)
Plasmid#29774DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only