Skip to main content
Addgene
Showing: 3261 - 3280 of 3401 results

  11. pcDNA4/TO/Strep-HA-ZAKalpha_deltaSdeltaCTD

    Plasmid
    #141198
    Purpose
    Expresses Strep-HA-ZAKalpha_deltaSdeltaCTD in mammalian cells, can be used to make inducible cell lines
    Depositor
    Simon Bekker-Jensen
    Article
    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20
    Insert
    MAP3K20 (MAP3K20 Human)
    Use
    Tags
    Strep, HA
    Expression
    Mammalian
    Mutation
    deletion of last 27 amino acids and amino acids 6…
    Promoter
    CMV
    Available since
    June 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141198-plasmid-map-sequence-id-300399

    Enlarge

  15. A3Ai E72A-Cas9n-UGI-NLS

    Plasmid
    #109430
    Purpose
    Expresses catalytically inactive human APOBEC3A with an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Reuben Harris
    Article
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A E72A Catalytic Mutant (APOBEC3A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    109430-plasmid-map-sequence-id-212945

    Enlarge

  19. pD649-HAsp-SEMA7A-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156922
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    SEMA7A (SEMA7A Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    156922-plasmid-map-sequence-id-302953

    Enlarge

  20. A3Bi-ctd E255A -Cas9n-UGI-NLS

    Plasmid
    #109429
    Purpose
    Expresses the C-terminal catalytically inactive mutant of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Reuben Harris
    Article
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    Apolipoprotein mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal Doman E255A Catalytic Mutant (APOBEC3B Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    109429-plasmid-map-sequence-id-212934

    Enlarge

Showing: 3261 - 3280 of 3401 results