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Plasmid#61938PurposeCo-expression of hUCH37 (ISF2) and hNFRKB (39-156) in E.coliDepositorUseTags6x HISExpressionBacterialMutationConstruct ends at residue 156, residues 2-38 dele…PromoterT7Available sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only
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pCD5-D/bovine/Nebraska/9-5/2012-HEFs57aED-GCN4-sfGFP-ST
Plasmid#175020PurposeExpresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFPDepositorInsertD/bovine/Nebraska/9-5/2012
UseTagsGCN4-TEV-sfGFP-TwinStrepExpressionMammalianMutations57a esterase knock out mutantPromoterCMVAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCD5-ss-D/bovine/France/5920/2014-HEFs57aED-GCN4-sfGFP-ST
Plasmid#175018PurposeExpresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFPDepositorInsertOK-HEF s57a ED
UseTagsGCN4-TEV-sfGFP-TwinStrepExpressionMammalianMutations57a esterase knock out mutantPromoterCMVAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCXIX_sh_HOIP/RNF31_#6
Plasmid#75252Purposeretroviral shRNA vector against human HOIP/RNF31, expresses GFP for transduction controlDepositorInsertshRNA against human RNF31 (RNF31 )
UseRNAi and RetroviralTagsExpressionMammalianMutationPromotermU6Available sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSuper-PACSIN1-shRNA
Plasmid#72576PurposeExpresses shRNA against PACSIN1DepositorInsertPACSIN1 (Pacsin1 Mouse)
UseRNAiTagsExpressionMammalianMutationPromoterH1 promoterAvailable sinceMay 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
UAS-u(XL)Cas9
Plasmid#127386PurposeExpresses Cas9 at very low levelDepositorInsertuORF(XL)-Cas9
UseCRISPRTagsExpressionInsectMutationPromoterUASTAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
DNMT1 KO Hyg
Plasmid#16648DepositorInsertDNMT1 knock-out construct (DNMT1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAdEasy-EGFP-CLASP2 340-1084
Plasmid#24524DepositorInsertCLASP2 (340-1084) (CLASP2 Human)
UseAdenoviralTagsEGFPExpressionMammalianMutationCLASP2 deletion mutant that retains MT lattice bi…PromoterAvailable sinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 FLAG mTOR 1968-2549 S2035T
Plasmid#23000DepositorInsertmTOR (MTOR Human)
UseTagsFLAGExpressionMammalianMutationS2035T aa 1968-2549 onlyPromoterAvailable sinceMay 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
integrin alpha9 pBS
Plasmid#13584DepositorInsertintegrin alpha 9 (ITGA9 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMiNGo11-3
Plasmid#126058PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cellDepositorInsertsmitochondria Rieske iron sulphur protein (1-100 aa)
ccdB cassette (BsaI)
GFP11x3 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMiNGo11-7
Plasmid#126057PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cellDepositorInsertsmitochondria Rieske iron sulphur protein (1-100 aa)
ccdB cassette (BsaI)
GFP11x7 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IL1RN gRNA3
Plasmid#113132PurposeExpresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)DepositorInsertIL1RN guideRNA 3 (SpCas9 scaffold), co-expressed GFP (transfection marker)
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
XE125 XDsh delta COOH-GFP-CS2+
Plasmid#16784DepositorInsertXdsh-delta-COOH (dvl2.L Frog)
UseXenopus expressionTagsGFPExpressionMutationcontains Dsh amino acids 1-588 (deleting 599-736)PromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
GTL-ir
Plasmid#81102PurposeGene Tagging vector LEU/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)DepositorInserttdiRFP
UseCre/LoxTagstdiRFPExpressionYeastMutationcodon optimized second iRFP to avoid internal rec…PromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETDUET1-hUCH37 (ISF1)-NFRKB(39-156)
Plasmid#61943PurposeCo-expression of hUCH37 (ISF1) and hNFRKB (39-156) in E.coliDepositorInsertsUseTags6x HISExpressionBacterialMutationResidues 2-39 deleted resulting in construct star…PromoterT7Available sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKM-U6-reRNA-mmSCN9A
Plasmid#205636PurposereRNA guide targeting Mus Musculus SCN9ADepositorInsertreRNA-mmSCN9A (Scn9a Mouse)
UseTagsExpressionMammalianMutationPromoterU6Available sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKM-U6-reRNA-acceptor-2xBbsI
Plasmid#205633PurposeCloning and U6-drive expression of ISDra2 TnpB reRNAsDepositorInsertISDra2-TnpB reRNA with placeholder for guide cloning with BbsI
UseTagsExpressionMammalianMutationPromoterU6Available sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)
Plasmid#218513Purposereplicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferaseDepositorInsertffluc
UseLuciferaseTagsExpressionBacterialMutationdeletion of the Plac_mrfp cassettePromoterPgyrA_ermBL-ermB'Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC3109 (pSpy1C-Plac(1)_ffluc)
Plasmid#218514Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseTagsExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(1)Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only